The validation of a solid phase clean-up procedure for the analysis of aflatoxins in groundnut cake using HPLC

Analytical methods for determination of mycotoxins: A review

“…As in most other examples referred to in the present review, sample pre-treatment plays a major part in the analysis. As described above this is normally done by LLE or by SPE depending on the matrix [53,78,81]. This section discusses different separation methods, their advantages and challenges in analysis and detection of several mycotoxins.…”

“…There have been uses of silica gel columns for purification of mycotoxins [81], ELISA for AFTs in corn and peanuts [89], C 2 , C 8 , C 18 and pH-bonded phases for AFT from maize [90], and pH-bonded cartridge from cottonseed [58]. There have been other recent clean up methods such as SPE [81], IAC [78], and SFE [53].…”

Rapid and Effective Sample Cleanup based on Polyvinylpyrrolidone-Coated Magnetite Nanoparticles Coupled with New Pre-Column Derivatization for Determination of Aflatoxins B1, B2, G1 and G2 in Animal Feeds by High-Performance Liquid Chromatography with Fluorescence Detection

“…In order to produce the highly fluorescent derivatives AFB 2a and AFG 2a from the less fluorescent AFB 1 and AFG 1 , respectively, the native fluorescence of AFB 1 and AFG 1 has been enhanced by different derivatization procedures: pre-column formation of hemiacetal derivates with trifluoracetic acid (TFA) or post-column derivatization with bromine or iodine (directly added or electrochemically cell produced). [24][25][26] Although these methods produce molecules with fluorescence intensity higher than their precursors, they also present several disadvantages. The major disadvantage of TFA derivatization is the low stability of AFB 1 and AFG 1 derivatives in methanol probably due to the formation of methyl acetals.…”

Functionalised Au Coated Iron Oxide Nanocomposites Based Reusable Immunosensor for AFB1 Detection

“…The European Commission has set the maximum permissible residue levels for AFB1 in corn products ready for retail sale at 2 g kg −1 [4][5][6][7]. Compared to conventional techniques [8][9][10], immunosensors are an interesting alternative that can be used to quantify and detect selectively toxin molecules [11][12][13][14]. Some of the biosensing techniques used for AFB1 detection include electrochemical biosensors [15,16], surface plasmon resonance [17,18], fluorescent biosensors [19,20], and quartz crystal (piezoelectric) microbalance based sensors [21,22].…”