The validation and application of the technicon H*1 for the complete automated evaluation of laboratory animal haematology

Davies, Daniel T.; Fisher, G

doi:10.1007/bf00422879

Cited by 22 publications

(13 citation statements)

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“…As LUC can be reactive lymphocytes, blasts, or monocytes, the absolute LUC count was added to automated lymphocyte (lymphocyte + LUC) or monocyte (monocyte + LUC) counts and these combined counts were compared with manual monocyte and lymphocyte counts. Inclusion of LUC in the monocyte count improved the association of the automated monocyte count with the manual count, and median monocyte counts were no longer significantly different between methods.…”

Section: Resultsmentioning

confidence: 99%

Reference intervals for hematologic and coagulation tests in adult alpacas (Vicugna pacos)

Dawson

DeFrancisco

Stokol

2011

Veterinary Clinical Pathol

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Section: Resultsmentioning

confidence: 99%

Reference intervals for hematologic and coagulation tests in adult alpacas (Vicugna pacos)

Dawson

DeFrancisco

Stokol

2011

Veterinary Clinical Pathol

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“…Comparison between HCT values obtained by the Technicon H*1 and those obtained using the microhematocrit method is shown in Figure 1. The correlation (r 2 ) in the iron‐treated group was .91 (n = 110) and .84 (n = 100) in the placebo group for samples collected at 1,2, 3, and 4 weeks after treatment (lambs 10–33 days of age) (data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…The Technicon H*1 automated hematology analyzer is a versatile and flexible diagnostic tool for many species 2–4 . It was useful for analyzing ovine blood and made it possible to diagnose anemia in young lambs by differentiating between the new and old populations of RBCs.…”

Section: Discussionmentioning

confidence: 99%

“…Because it displays a distribution of all cells analyzed, it enables the user to detect shifts too small to influence the mean values 1 . The H*1 has been a useful and reliable diagnostic tool for several species, 2–4 but studies on ovine blood have not been reported. Software specific for analyzing rat, mouse, dog, rabbit, monkey, horse, and cat blood has been developed.…”

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Hematologic Evaluation of Normal and Anemic Lambs with the Technicon H*1 Using EDTA or Heparin as Anticoagulants

Vatn

Framstad

Torsteinbø

2000

Veterinary Clinical Pathol

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A study was performed to evaluate blood from young lambs using the Technicon H*1 hematology analyzer, with emphasis on RBC parameters, comparison of tripotassium EDTA and heparin, and the effects of storage on heparinized blood. Blood samples from lambs 2 days to 18 weeks of age were analyzed within 6 hours, revealing a high precision, except for WBC counts in heparinized blood. The HCT values estimated by the H*1 correlated well (r(2)=.90) with those obtained by the microhemotocrit method. Mean hematologic values obtained for heparinized blood differed by up to 4% from values obtained from blood collected into EDTA. WBC counts decreased 8.5% in heparinized blood stored at 4 degrees C for 1 day, but differences observed in RBC counts were

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“…Any bias between the two methods was evaluated for each sample and the result reported as the percentage difference with respect to the manual method. The data were also used to calculate Sensitivity Ratio (SR) (Davies and Fisher, 1991). This ratio takes account of the slope of the linear regression and the analytical error or variability associated with each method.…”

Section: Discussionmentioning

confidence: 99%

A Comparative Study of the Ovine Haemogram: Cell-Dyn 3500 Versus Manual Methods

Athanasiou¹

2013

American Journal of Animal and Veterinary Sciences

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Manual hematology techniques traditionally applied in farm animal medicine are time consuming and laborintensive, especially when large numbers of samples have to be processed. As a result, several automated hematology instruments have been developed for use in these species. An automated hematology analyzer (Abbott Cell-Dyne 3500 system) was used in performing a complete blood cell count and differential counts of white blood cells in sheep blood samples. The system was compared with basic manual hematologic techniques. A linear regression was used to assess correlation between the two methods. Correlation coefficients (R2) were good for the hematocrit, the total white blood cell count, the neutrophils, the lymphocytes and the platelet count, while a poor correlation existed in monocytes, eosinophils and basophils. The automated and the manual technique were also compared in terms of sensitivity using the Sensitivity Ratio (SR). The automated analyzer was slightly more sensitive than the manual technique for all parameters tested except for monocytes, eosinophils and basophils were the difference was greater and the automated analyzer was 1.5, 2.5 and 2 times more reliable, respectively. The method bias was also calculated. It seems that the overall performance of the automated analyzer justifies its utilization in sheep blood analysis, although as for any analyzer used in any species, a stained blood film evaluation remains an indispensable technique to confirm the results being reported by the automated analyzer and provide additional information for the ovine haemogram.

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The validation and application of the technicon H*1 for the complete automated evaluation of laboratory animal haematology

Cited by 22 publications

References 1 publication

Reference intervals for hematologic and coagulation tests in adult alpacas (Vicugna pacos)

Reference intervals for hematologic and coagulation tests in adult alpacas (Vicugna pacos)

Hematologic Evaluation of Normal and Anemic Lambs with the Technicon H*1 Using EDTA or Heparin as Anticoagulants

A Comparative Study of the Ovine Haemogram: Cell-Dyn 3500 Versus Manual Methods

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