Hematologic Evaluation of Normal and Anemic Lambs with the Technicon H*1 Using EDTA or Heparin as Anticoagulants

Abstract

A study was performed to evaluate blood from young lambs using the Technicon H*1 hematology analyzer, with emphasis on RBC parameters, comparison of tripotassium EDTA and heparin, and the effects of storage on heparinized blood. Blood samples from lambs 2 days to 18 weeks of age were analyzed within 6 hours, revealing a high precision, except for WBC counts in heparinized blood. The HCT values estimated by the H*1 correlated well (r(2)=.90) with those obtained by the microhemotocrit method. Mean hematologic values obtained for heparinized blood differed by up to 4% from values obtained from blood collected into EDTA. WBC counts decreased 8.5% in heparinized blood stored at 4 degrees C for 1 day, but differences observed in RBC counts were </= 2%. Problems occurred when analyzing blood from young lambs with low hemoglobin values because the H*1 incorrectly counted microcytes with volumes of < 10 fL as platelets. When the necessary corrections were performed, the H*1 was useful for analyzing RBC parameters in lamb blood collected both into EDTA and into heparin.

Intervalos de referência sanguíneos e a influência da idade e sexo sobre parâmetros hematológicos e bioquímicos de ovinos da raça Santa Inês criados na Amazônia Oriental

“…Entretanto, em ovinos são escassos os trabalhos que descrevem resultados para esses índices, principalmente em animais saudáveis, caso do presente estudo. Só foram encontrados trabalhos com descrição de valores de RDW em cordeiros com alimentação deficiente em ferro (Vatn et al 2000) e em ovelhas durante a gestação (Fazio et al 2011).…”

Clinical Chemistry of Companion Avian Species: A Review

“…Experienced avian veterinarians working with experienced restrainers, who minimize trauma to the vessel wall, can collect a high quality sample without the addition of heparin. Reducing the amount of heparin in the sample is desirable when using automated hematology analyzers, such as the Cell Dyn 3500 (Abbott Laboratories, Abbott Park, Ill) because of a decreased coefficient of variation of the WBC count 1 as well as decreasing potential dilution and assay artifact in a biochemical sample. If most of the heparin is expelled from the needle hub, it will minimally affect the sample.…”

Haematology values and effect of age and reproductive stage on haematological parameters of Chios sheep

“…Haematocrit is increased in birth, declines up to the 14th day of life, partially because of iron deficiency (Ullrey et al 1965a, Schalm 1986, and after day 14 increases continuously Στην παρούσα μελέτη καταβλήθηκαν προσπάθειες ώστε να αποφευχθεί η επίδραση παραγόντων, όπως το αντιπηκτικό, η αποθήκευση των δειγμάτων, οι βελόνες και οι χειρισμοί της αιμοληψίας, στα αποτελέσματα των αιματολογικών εξετάσεων. Ως αντιπηκτικό χρησι μοποιήθηκε EDTA, το οποίο είναι καλύτερο από την ηπαρίνη και το κιτρικό νάτριο για τη μελέτη των έμ-μορφων κυτταρικών στοιχείων του αίματος (Vatn et al 2000, Morris 2002a). Τα σωληνάρια κενοΰ γεμίζονταν πλήρως κατά την αιμοληψία, προκειμένου να υπάρχει σωστή αναλογία αίματος-αντιπηκτικοΰ, καθώς, όταν το EDTA είναι σε μεγαλύτερη αναλογία από το κανονικό, προκαλείται συρρίκνωση των ερυθρών αιμοσφαιρίων και μείωση του αιματοκρίτη (Morris 2002a).…”

“…EDTA is preferred for the complete blood count compared to heparin and sodium citrate (Vatn et al 2000, Morris 2002a). Vacuum glass tubes were filled with the proper amount of blood, in order the correct analogy of blood and anticoagulant to exist.…”