The utilization of sugar cane molasses with/without the presence of lignosulfonate for the production of bacterial cellulose

Keshk, Sherif M. A. S.; Sameshima, Kazuhiko

doi:10.1007/s00253-005-0265-6

Cited by 102 publications

(55 citation statements)

References 19 publications

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“…Thus, it was confirmed that the medium compositions gave some influence on the crystalline phase of the BC. These results agreed with those of Keshk and Sameshima who found that use of molasses resulted in a small decrease in crystallinity index of BC produced by A. xylinum strains [33]. On the other hand, Watanabe et al [34] and Iwata et al [35] reported that BC produced in agitated culture had lower crystallinity index than that in static culture.…”

Section: X-ray Diffractometrysupporting

confidence: 91%

Production and Characterization of Cellulose by Acetobacter sp. V6 Using a Cost-Effective Molasses–Corn Steep Liquor Medium

Jung

Lee

Jeong

et al. 2009

Appl Biochem Biotechnol

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In order to reduce of the manufacturing cost of bacterial cellulose (BC), BC production by Acetobacter sp. V6 was investigated in shaking culture using molasses and corn steep liquor (CSL) as the sole carbon and nitrogen sources, respectively. The highest BC production was obtained with Ca3(PO4)2-treated molasses. Maximum BC yield (2.21+/-0.04 g/l) was obtained at 5% (w/v) total sugar in molasses. In improved medium containing molasses and CSL, BC production was observed in the medium after 1 day of incubation and increased rapidly thereafter with maximum yield (3.12+/-0.03 g/l) at 8 days. This value was approximately twofold higher than the yield in the complex medium. Physical properties of BC from the complex and molasses media were studied using Fourier-transform infrared (FT-IR) spectroscopy and X-ray diffractometer. By FT-IR, all the BC were found to be of cellulose type I, the same as typical native cellulose. The relative crystallinity of BC produced in the complex and molasses media were 83.02 and 67.27%, respectively. These results suggest that molasses and CSL can be useful low-cost substrates for BC production by Acetobacter sp. V6 without supplementation with expensive nitrogen complexes such as yeast extract and polypeptone, leading to the reduction in the production costs.

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Section: X-ray Diffractometrysupporting

confidence: 91%

Production and Characterization of Cellulose by Acetobacter sp. V6 Using a Cost-Effective Molasses–Corn Steep Liquor Medium

Jung

Lee

Jeong

et al. 2009

Appl Biochem Biotechnol

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“…Further as mentioned earlier, there is a tendency to use many different substrates/agricultural byproducts (cane molasses, beet molasses, pineapple skin, cheese whey permeate, high solids potato effluents for the manufacture of nata-de-coco (Keshk and Sameshima 2006). Under such circumstances there is a greater need for standardization of the manufacturing process and for stricter controls/specifications.…”

Section: Resultsmentioning

confidence: 96%

The effect of pH, sucrose and ammonium sulphate concentrations on the production of bacterial cellulose (Nata-de-coco) by Acetobacter xylinum

Jagannath

Kalaiselvan

Manjunatha

et al. 2008

World J Microbiol Biotechnol

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The effect of pH, sucrose and ammonium sulphate concentrations on the production of nata-de-coco, a form of bacterial cellulose, by Acetobacter xylinum was studied. Comparisons for physical properties like thickness, wet weight, water-holding capacity (WHC), moisture content and hardness, a textural parameter were done on nata-de-coco grown in tender coconut water medium supplemented with varying concentrations of sucrose and ammonium sulphate at different pH values. The results were analysed by fitting a second-order polynomial regression equation. Response surface methodology was used to study the effect of the three variables. The study showed that A. xylinum could effectively use sucrose as the sole carbon source in coconut water medium and that cellulose production was more dependent on pH than either sucrose or ammonium sulphate concentrations. Maximum thickness of nata was obtained at pH 4.0 with 10% sucrose and 0.5% ammonium sulphate concentrations. These conditions also produced good quality nata-de-coco with a smooth surface and soft chewy texture. The study will enable efficient utilization of coconut water, a hitherto wasted byproduct of coconut industry and will also provide a new product dimension to the aggrieved coconut farmers who are not getting the right price for their product.

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“…Of all, adding appropriate stimulatory factors has received much attention for its simplicity and already observed significant effects on BC production. At present, studies on stimulatory factors mainly focus on ethanol (Naritomi et al 1998a), organic acids (Embuscado et al 1994;Toda et al 1997;Naritomi et al 1998b), Water-Soluble Polysaccharides (Bae et al 2004;Ishida et al 2003), and lignin sulfonate (Keshk and Sameshima 2006). Among them, ethanol has been shown to be a good stimulatory factor for BC production, and the underlying mechanism has also been investigated (Yunoki et al 2004).…”

Section: Introductionmentioning

confidence: 97%

Effects of alcohols on bacterial cellulose production by Acetobacter xylinum 186

Zhang

Chi

et al. 2011

World J Microbiol Biotechnol

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To improve the yield of cellulose production in bacteria, we investigated the stimulatory effects of six different alcohols during fermentation of Acetobacter xylinum 186. Our study showed that after static fermentation at 30°C for 6 days, bacterial culture with 1.0% (v/v) of methanol added in the medium produced the highest bacterial cellulose (BC) yield at 103.5 mg/100 ml, which was 21.8% higher than the control group. Addition of 0.5% of ethylene glycol in the culture yielded 105.5 mg/100 ml BC, 24.1% higher than the control group. Adding 0.5% of n-propanol yielded 96.4 mg/100 ml BC, 13.4% higher; 3.0% of glycerol yielded 108.3 mg/100 ml BC, 27.4% higher; 0.5% of n-butanol yielded 132.6 mg/100 ml BC, 56.0% higher; and 4.0% of mannitol in the culture yielded 125.2 mg/100 ml BC, 47.3% higher, respectively. The rate of bacterial cellulose production increased with the growth rate of the bacteria. The stimulatory effects of these alcohols that we observed were significant in the later stage of fermentation, which was considered to be important for the biosynthesis of bacterial cellulose.

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The utilization of sugar cane molasses with/without the presence of lignosulfonate for the production of bacterial cellulose

Cited by 102 publications

References 19 publications

Production and Characterization of Cellulose by Acetobacter sp. V6 Using a Cost-Effective Molasses–Corn Steep Liquor Medium

Production and Characterization of Cellulose by Acetobacter sp. V6 Using a Cost-Effective Molasses–Corn Steep Liquor Medium

The effect of pH, sucrose and ammonium sulphate concentrations on the production of bacterial cellulose (Nata-de-coco) by Acetobacter xylinum

Effects of alcohols on bacterial cellulose production by Acetobacter xylinum 186

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