The utility of the hematoxylin and eosin staining in patients with suspected Hirschsprung disease

Setiadi, Josephine Amanda; Dwihantoro, Andi; Iskandar, Kristy; Heriyanto, Didik Setyo; Gunadi, Gunadi

doi:10.1186/s12893-017-0267-1

Cited by 20 publications

(20 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, our study did not aim to determine the accuracy of contrast enema for diagnosis of HSCR since all HSCR patients in our hospital were diagnosed using full thickness biopsy samples stained by HE and S100 [11]. Our study's focus was on prediction of intraoperative aganglionosis level using contrast enema for HSCR patients with short-segment aganglionosis who underwent TEPT, since most HSCR patients are short-segment aganglionosis and currently they often undergo TEPT [2].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Accuracy of transition zone in contrast enema to predict intraoperative aganglionosis level in patients with Hirschsprung disease

et al. 2020

Self Cite

View full text Add to dashboard Cite

Objective: While frozen section methods have been widely conducted to determine aganglionosis segment during transanal endorectal pull-through (TEPT) for Hirschsprung disease (HSCR) patients in most institutions, some hospitals still rely on contrast enema to predict aganglionosis segments due to unavailability of frozen section facilities. We determined the accuracy of transition zone in contrast enema to predict aganglionosis segments during TEPT. We retrospectively reviewed all contrast enema and frozen sections for HSCR patients under 2 years of age who underwent TEPT at our institution. Results: We recruited 36 HSCR patients: twenty-six patients (72.2%) had radiographic transition zones limited to rectum, while ten subjects (27.8%) were limited to rectosigmoid. The rectum subgroup of patients showed a concordance of 30.8%, whereas the rectosigmoid subgroup had a concordance of 100%. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of contrast enema compared with intraoperative histopathological findings for aganglionosis level were 100% (95% CI 0.60-1.0), 35.7% (95% CI 0.19-0.56), 30.8% (95% CI 0.15-0.52), 100% (95% CI 0.66-1.0), and 50% (95% CI 0.33-0.67), respectively. In conclusions, contrast enema has low accuracy to predict intraoperative aganglionosis segments in HSCR patients, indicating that it might not be utilized to determine aganglionosis level during TEPT.

show abstract

Section: Discussionmentioning

confidence: 99%

“…1). We utilized hematoxylin and eosin (HE) as staining for intraoperative histopathological evaluation [11] (Additional file 1: Fig. S1).…”

Section: Intraoperative Histopathological Findings and Contrast Enemamentioning

confidence: 99%

Accuracy of transition zone in contrast enema to predict intraoperative aganglionosis level in patients with Hirschsprung disease

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Even though immunohistochemistry techniques are widely used for the diagnosis of HD in developed countries, hematoxylin–eosin staining presents a moderate reliability for this diagnosis, but it can be used in case of the lack of more expensive immunohistochemistry techniques. [ 19 ] A recent study underlined that the histological examination of the appendix is a very useful tool in the diagnosis of TCA. [ 13 ]…”

Section: Discussionmentioning

confidence: 99%

Atypical onset of total colonic Hirschsprung disease in a small female infant

Mărginean

Meliţ²,

Gozar³

et al. 2018

Medicine

View full text Add to dashboard Cite

Rationale:Hirschsprung disease (HD) or colonic aganglionosis is a congenital disorder, which results from the abnormal migration of neuronal cells of the neural crest leading to a disorder of the enteric nervous system consisting in the absence of ganglion cells within the submucosal and myenteric plexus.Patient concerns:We report the case of a 7-month-old female infant admitted in our clinic for constipation and failure to thrive. At the age of 6 months, she was examined in our clinic for the same reasons, and we recommended symptomatic treatment without improvements. The clinical examination revealed pallor of the skin and mucosa, distended abdomen, and abdominal tenderness at palpation.Diagnoses:The abdominal ultrasound showed abdominal bloating, and the barium enema was normal. The patient's evolution worsened progressively within the following 3 days after admission associating sings of toxic megacolon.Interventions:She underwent a surgical intervention with total colectomy and ileostomy, and the final diagnosis confirmed by the histological examination was of total colonic aganglionosis (TCA).Outcomes:The evolution immediately after the surgery and the follow-up examination after approximately 3 months pointed out normal weight gain and the laboratory tests were within normal limits.Lessons:TCA can also manifest in older infants. Barium enemas can guide the diagnosis in most cases of HD. Nevertheless, in patients with TCA, it can be normal. Moreover, it could represent a trigger for toxic megacolon.

show abstract

“…We conducted a retrospective study of infants with BA at the Pediatric Surgery Division, Department of Surgery, Dr. Sardjito Hospital in Yogyakarta, Indonesia from January 2012 to January 2016. Dr. Sardjito Hospital is a University Teaching Hospital and as a tertiary referral center, it serves urban and rural populations from Yogyakarta province [ 13 – 15 ].…”

Section: Main Textmentioning

confidence: 99%

Liver transplant score for prediction of biliary atresia patients’ survival following Kasai procedure

et al. 2018

Self Cite

View full text Add to dashboard Cite

ObjectivesRecently, a scoring system has been developed to predict which patients with biliary atresia (BA) who underwent a Kasai procedure should be considered for liver transplant. Here, we applied the scoring system to predict the survival of BA patients following the Kasai procedure at Dr. Sardjito Hospital, Yogyakarta, Indonesia from January 2012 to January 2016.ResultsThere were 26 patients, of whom 14 were males and 12 females. Outcomes of BA patients after the Kasai surgery were 15 survived and 11 died. There were significant associations between ascites and sepsis with the liver transplant score of ≥ 8 (p value = 0.006 and 0.014, respectively), whereas post-operative bilirubin level, ALT level, prothrombin time, cirrhosis, esophageal varices, portal hypertension, and cholangitis did not significantly correlate to the score. The patients with a score ≥ 8 have a relatively greater risk by 3.5-fold to die compared with patients with a score < 8, but it did not reach a significant level (p value = 0.13). In conclusions, the incidence of ascites and sepsis might predict the poor prognosis of BA patients following the Kasai procedure. Moreover, patients with a score ≥ 8 are prone to die after the Kasai surgery if they do not undergo a liver transplant.

show abstract

The utility of the hematoxylin and eosin staining in patients with suspected Hirschsprung disease

Cited by 20 publications

References 20 publications

Accuracy of transition zone in contrast enema to predict intraoperative aganglionosis level in patients with Hirschsprung disease

Accuracy of transition zone in contrast enema to predict intraoperative aganglionosis level in patients with Hirschsprung disease

Atypical onset of total colonic Hirschsprung disease in a small female infant

Liver transplant score for prediction of biliary atresia patients’ survival following Kasai procedure

Contact Info

Product

Resources

About