The utility of RAPD markers for the determination of genetic variation in oil palm (Elaeis guineensis)

Shah, Farida Habib; Simons, A. J.; Dunsdon, A.J.

doi:10.1007/bf00223710

Cited by 67 publications

(29 citation statements)

References 6 publications

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“…Among the likely alternatives, isozymes are not satisfactorily variable due to low polymorphism [23] [24]. Random amplified polymorphic DNA (RAPD) has also been examined [25], but poor reproducibility of amplification products limits their generalisation in genetic diversity studies [26]. Other more robust molecular markers such as restriction fragment length polymorphic DNA (RFLP) [27] are complex; requiring relatively large amounts of purified and high molecular weight DNA, time-consuming and laborious.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Diversity of NIFOR Oil Palm Main Breeding Parent Genotypes Using Microsatellite Markers

Okoye¹,

Uguru²,

Bakoumé³

et al. 2016

AJPS

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The genetic diversity among 15 NIFOR breeding parents was assessed using 10 microsatellite markers. A high genetic diversity was observed with a total of 64 alleles including 23 rare alleles or alleles at frequencies less than 0.05. The NIFOR tenera parents recorded the highest number of rare alleles. The average observed heterozygosity and mean gene diversity across all parental groups were 0.6889 and 0.7029, respectively. Higher genetic diversity was detected among the NIFOR dura and tenera parents compared to that of the Deli dura parents in absolute terms. Analysis of molecular variance (AMOVA) showed that 87% of the total variation (p < 0.001) observed was due to differences among parents. Rogers' genetic distance ranged from 0.2988 to 0.8000 (mean = 0.5570). The dendrogram constructed on the basis of Rogers' genetic distance clustered the parents in three groups. They generally clustered in heterotic manner rather than by geographic origins. The groupings obtained through PCoA confirmed the results obtained by cluster analysis. The results obtained are strong assets for NIFOR breeding programme.

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Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Diversity of NIFOR Oil Palm Main Breeding Parent Genotypes Using Microsatellite Markers

Okoye¹,

Uguru²,

Bakoumé³

et al. 2016

AJPS

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“…Using molecular marker to screen the germplasm has been limited to only a few studies using random amplified polymorphic DNA (RAPD; Shah et al 1994), isozymes (Hayati et al 2004), restriction fragment length polymorphism (RFLP; Maizura et al 2006) and amplified fragment length polymorphism (Kularatne 2000). Recently Bakoume (2006) used the genomic simple sequence repeat (SSR) markers developed for oil palm by Billotte et al (2001) to extensively screen the Cameroon collection.…”

Section: Introductionmentioning

confidence: 99%

Exploiting an oil palm EST database for the development of gene-derived SSR markers and their exploitation for assessment of genetic diversity

et al. 2008

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A total of 5,521 expressed sequence tags (ESTs) from oil palm were used to search for type and frequency of simple sequence repeat (SSR) markers. Dimeric repeat motifs appeared to be the most abundant, followed by tri-nucleotide repeats. Redundancy was eliminated in the original EST set, resulting in 145 SSRs in 136 unique ESTs (114 singletons and 22 clusters). Primers were designed for 94 (69.1%) of the unique ESTs (consisting of 14 consensus and 80 singletons). Primers for 10 EST-SSRs were developed and used to evaluate the genetic diversity of 76 accessions of oil palm originating from seven countries in Africa, and the standard Deli dura population. The average number of observed and effective alleles was 2.56 and 1.84, respectively. The EST-SSR markers were found to be polymorphic with a mean polymorphic information content value of 0.53. Genetic differentiation (FST) among the populations studied was 0.2492 indicating high level of genetic divergence. Moreover, the UPGMA (unweighted pair-group method with arithmetic mean) analysis revealed a strong association between genetic distance and geographic location of the populations studied. The germplasm materials exhibited higher diversity than Deli dura, indicating their potential usefulness in oil palm improvement programmes. The study also revealed that the populations from Nigeria, Congo and Cameroon showed the highest diversity among the germplasm evaluated in this study. The EST-SSRs further demonstrated their worth as a new source of polymorphic markers for phylogenetic analysis, since a high percentage of the markers showed transferability across species and palm taxa.

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“…Molecular markers have been used for different applications like genetic diversity, genotype identification, QTL mapping and marker assisted selection. Assessment of the genetic variation and diversity in oil palm has been carried out based on RAPD (Shah et al, 1994;Rajanaidu et al, 2000;Satish and Mohan Kumar, 2007), AFLP (Kularatne, 2002), Isozymes (Hayati et al, 2004) and RFLP (Maizura et al, 2006). Among these, PCR based SSRs are widely used in any crop improvement programmes and also in oil palm germplasm.…”

Section: Issn: 2319-7706 Volume 7 Number 04 (2018)mentioning

confidence: 99%

Identification and Utilization of Polymorphic SSR Markers for Genetic Diversity Studies in Oil Palm

Bhagya¹,

Babu²,

Naika³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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The utility of RAPD markers for the determination of genetic variation in oil palm (Elaeis guineensis)

Cited by 67 publications

References 6 publications

Assessment of Genetic Diversity of NIFOR Oil Palm Main Breeding Parent Genotypes Using Microsatellite Markers

Assessment of Genetic Diversity of NIFOR Oil Palm Main Breeding Parent Genotypes Using Microsatellite Markers

Exploiting an oil palm EST database for the development of gene-derived SSR markers and their exploitation for assessment of genetic diversity

Identification and Utilization of Polymorphic SSR Markers for Genetic Diversity Studies in Oil Palm

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