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The field experiment was conducted to study suitable production technologies to maximise yield of sesame during the year 2004-2005. The experiment was conducted in randomised block design with thirteen treatments and replicated thrice. The treatment comprises of control, recommended dose of NPK, organic matter, application of biofertilizer, micronutrients, clipping at (0+ 2) leaf stage and planofix spraying of 30 ppm at 45 DAS and 55 DAS with different combinations. Among the treatment T 12 (Azospirillum seed treatment, RD of NPK, soil application of MnSO 4 @ 5 kg ha -1 , clipping at 0 + 2 leaf stage and planofix spraying of 30 ppm at 45 DAS and at 55 DAS) has recorded maximum number of capsules per plant (91.12), number of seeds per capsule (69.82), seed yield (1160.75 kg ha -1 ), stover yield (3133.61 kg ha -1 ) and harvest index (27.02%). Hence, application of Azospirillum as seed treatment, recommended dose of NPK, soil application of MnSO 4 @ 5 kg ha -1 , clipping at 0 + 2 leaf stage and planofix spraying of 30 ppm at 45 DAS and at 55 DAS found to be the best production technologies to improve yield.

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The availability of large expressed sequence tag (EST) and whole genome databases of oil palm enabled the development of a data base of microsatellite markers. For this purpose, an EST database consisting of 40,979 EST sequences spanning 27 Mb and a chromosome-wise whole genome databases were downloaded. A total of 3,950 primer pairs were identified and developed from EST sequences. The tri and tetra nucleotide repeat motifs were most prevalent (each 24.75%) followed by di-nucleotide repeat motifs. Whole genome-wide analysis found a total of 245,654 SSR repeats across the 16 chromosomes of oil palm, of which 38,717 were compound microsatellite repeats. A web application, OpSatdb, the first microsatellite database of oil palm, was developed using the PHP and MySQL database (https://ssr.icar.gov.in/index.php). It is a simple and systematic web-based search engine for searching SSRs based on repeat motif type, repeat type, and primer details. High synteny was observed between oil palm and rice genomes. The mapping of ESTs having SSRs by Blast2GO resulted in the identification of 19.2% sequences with gene ontology (GO) annotations. Randomly, a set of ten genic SSRs and five genomic SSRs were used for validation and genetic diversity on 100 genotypes belonging to the world oil palm genetic resources. The grouping pattern was observed to be broadly in accordance with the geographical origin of the genotypes. The identified genic and genome-wide SSRs can be effectively useful for various genomic applications of oil palm, such as genetic diversity, linkage map construction, mapping of QTLs, marker-assisted selection, and comparative population studies.