The utility of cellient cell blocks in low‐cellularity thyroid fine needle aspiration biopsies

Horton, Michelle; Been, Laura; Starling, Cherry; Traweek, S. Thomas

doi:10.1002/dc.23522

Cited by 16 publications

(8 citation statements)

References 13 publications

(21 reference statements)

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“…The majority of our cases showed cellular cell block material with only a few with borderline adequate material. Our findings are essentially in agreement with diagnostic utility of cell block material in previous reported studies . We have also noticed that presence of adequate material on pap stained slides along with the cellular material on cell block enhance the diagnostic details and complement the characterization in to a definitive TBSRTC.…”

Section: Discussionsupporting

confidence: 92%

“…Cristo et al in a study of 358 patients compared cytology and combined cytology cell block nondiagnostic rates and found 17.1% vs 4.3%, respectively . Others have also found that the use of cell block material yields diagnostic material in 31% of nondiagnostic ThinPrep . Similarly, we compared nondiagnostic rates between cytology (without cell block) and combined cytology and cell block and found comparable values, 16.4% vs 9.3%, respectively.…”

Section: Discussionsupporting

confidence: 60%

“…In order to decrease the nondiagnostic cytology result rate, it is important to have adequate material on the cytology sides. Value of the cell block material in thyroid FNA has also been evaluated . Cristo et al in a study of 358 patients compared cytology and combined cytology cell block nondiagnostic rates and found 17.1% vs 4.3%, respectively .…”

Section: Discussionmentioning

confidence: 99%

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Diagnostic utility of cell block in fine needle aspiration cytology of thyroid gland

Vance

Durbin

Manglik

et al. 2019

Diagnostic Cytopathology

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Background: This study was conducted to evaluate the diagnostic utility of cell block material in fine needle aspiration (FNA) of thyroid nodules.Design: A total of 242 thyroid fine need aspirations (FNAs) were performed between January 2015 and December 2015. Of those, all consecutive thyroid FNA cases with cell blocks (n = 140) from 129 patients (age: 58.9 ± 12.8 years) are included in this study.Cytology slides and cell blocks are reviewed for adequacy assessment based on the Bethesda System for Reporting Thyroid Cytopathology (TBSRTC) and then categorizing them into TBSRTC diagnostic categories. These cases are divided into two groups, combined cytology and cell block (C + CB) and cytology without cell block (C).Results: In the first group (C + CB), a total 140 cases are categorized in TBSRTC as follows: I: 13 (9.3%) cases, II: 78 (55.7%) cases, III: 7 (5%), IV: 16 (11.4%), V: 3(2.2%) and VI: 23 (16.4%). In the second group (C), the cases are classified in TBSRTC as follows: I: 23 (16.4%) cases, II: 70 (50%), III: 7 (5%), IV: 16 (11.4%), V: 3 (2.2%) and VI: 21 (15%). Nondiagnostic rate was 7.1% lower in the first group (C + CB) as compared with second group (C) (First group: 9.3% vs second group: 16.4%, P = .0764).Conclusions: Combined use of cytology slides and cell block decreases the nondiagnostic rate up to 7.1% as compared with cytology without cell block.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

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Diagnostic utility of cell block in fine needle aspiration cytology of thyroid gland

Vance

Durbin

Manglik

et al. 2019

Diagnostic Cytopathology

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“…The material remaining after the slide preparation has been completed can be used for the application of immunocytochemistry on cell block material as well as for other ancillary techniques. The application of the cell block technique on thyroid FNA material has been described in the literature as having very good results, particularly in cases of unsatisfactory or intermediate cytology [15,16]. …”

Section: Discussionmentioning

confidence: 99%

Application of Immunocytochemistry on Cell Block Sections for the Investigation of Thyroid Lesions

Margari

Giovannopoulos²,

Pouliakis³

et al. 2018

Acta Cytologica

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Objective: To investigate the potential of Classification and Regression Trees (CARTs) for the diagnosis of thyroid lesions based on cell block immunocytochemistry and cytological outcome. Study Design: A total of 956 histologically confirmed cases (673 benign and 283 malignant) from patients with thyroid nodules were prepared via liquid-based cytology and evaluated; 4 additional slides were stained for cytokeratin 19 (CK-19), galectin 3 (Gal-3), Hector Battifora mesothelial cell 1 (HBME-1), and thyroglobulin. On the basis of immunocytochemistry and the cytological diagnosis, a CART algorithm was constructed and used for evaluation. Results: The major important factors contributing to the diagnostic CART model were: cytological outcome, CK-19, Gal-3, and HBME-1. The sensitivity and specificity of the cytological diagnosis were 96.27% and 88.26%, respectively (cut-off: category 3 of The Bethesda System [TBS-3]). The introduction of immunocytochemistry and the CART model increased the sensitivity and specificity to 98.88% and 99.11%, respectively. CK-19 presented the best performance for discriminating papillary thyroid carcinomas, followed by HBME-1 and Gal-3. In the TBS-2 cases, CK-19 and, subsequently, Gal-3 were important immunocytochemistry markers. Ultimately, CK-19 and HBME-1 on TBS-5 or TBS-6 cases demonstrated the best results. Conclusions: The hierarchical structure of the CART model provides a diagnostic algorithm linked with the risk of malignancy at every step of the procedure. It also provides guidance on the use of ancillary examinations as it goes by simple, human understandable rules.

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“…Both alcohol and formalin fixation are possible with morphology and cellularity seem to be superior to traditional CB techniques . For thyroid specimens, automated methods are preferable in the case of nondiagnostic and atypia of undetermined significance/follicular lesion of undetermined significance results, but yield little additional information in the case of low‐cellularity specimens . Importantly, immunocytochemical protocols for both manual and automated methods should be optimised and adapted for formalin or alcohol‐fixed specimens …”

Section: Cell Block (Cb) Preparationmentioning

confidence: 99%

Management of thyroid cytological material, preanalytical procedures and bio‐banking

et al. 2018

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Thyroid nodules are common and are increasingly detected due to recent advances in imaging techniques. However, clinically relevant thyroid cancer is rare and the mortality from aggressive thyroid cancer remains constant. Fine needle aspiration cytology (FNAC) is a standard method for diagnosing thyroid malignancy and the discrimination of malignant nodules from goitre. As the examined nodules on thyroid FNAC are often small incidental findings, it is important to maintain a low rate of undetermined diagnoses requiring further clinical work up or surgery. The most important factors determining the accuracy of the cytological diagnosis and suitability for biobanking of thyroid FNACs are the quality of the sample and availability of adequate tissue for auxiliary studies. This article discusses technical aspects (preanalytics) of performing thyroid FNAC, including image guidance and rapid on-site evaluation, sample collection methods (conventional slides, liquid-based methods, cell blocks) and storage (bio-banking). The spectrum of special studies (immunocytochemistry on direct slides or liquid-based cytology, immunohistochemistry on cell blocks and molecular methods) required for improving the precision of the cytological diagnosis of the thyroid nodules is also discussed.

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The utility of cellient cell blocks in low‐cellularity thyroid fine needle aspiration biopsies

Cited by 16 publications

References 13 publications

Diagnostic utility of cell block in fine needle aspiration cytology of thyroid gland

Diagnostic utility of cell block in fine needle aspiration cytology of thyroid gland

Application of Immunocytochemistry on Cell Block Sections for the Investigation of Thyroid Lesions

Management of thyroid cytological material, preanalytical procedures and bio‐banking

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