The usefulness of the deconvolution of chromatograms into orthogonal polynomials for characterizing the quality of separation

Non-parametric linear regression of discrete Fourier transform convoluted densitometric peak responses

“…Deconvolution of chromatograms into orthogonal polynomials has been applied for characterizing the quality of separation in patterns of strongly overlapping peaks, e.g., sulpha drugs analyzed by reversed phase HPLC column [3]. Analysis of enantiomers giving partially overlapping peaks was also made possible by using different chemometric treatments of chromatographic ultraviolet signals, e.g., quantification of pseudoephedrine enantiomers [4].…”