The use of zebrafish (Danio rerio) as biomedical models

Teame, Tsegay; Zhang, Zhen; Ran, Chao; Zhang, Hongling; Yang, Yalin; Ding, Qianwen; Xie, Minxu; Gao, Chenchen; Ye, Yongan; Duan, Ming; Zhou, Zhigang

doi:10.1093/af/vfz020

Cited by 166 publications

(111 citation statements)

References 48 publications

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“…The zebrafish model continues to increase in popularity (Teame et al, 2019), while recent advances in genome editing technologies lower experimental barriers for non-traditional models. Our adaptable behavioral setup can monitor any small aquatic organism, particularly in multi-well format, and can thus accelerate discovery along both of these avenues.…”

Section: Discussionmentioning

confidence: 99%

A Customizable Low-Cost System for Massively Parallel Zebrafish Behavior Phenotyping

Joo

Vivian

Graham

et al. 2020

Preprint

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High-throughput behavioral phenotyping is critical to genetic or chemical screening approaches. Zebrafish larvae are amenable to high-throughput behavioral screening because of their rapid development, small size, and conserved vertebrate brain architecture. Existing commercial behavior phenotyping systems are expensive and not easily modified for new assays. Here, we describe a modular, highly adaptable, and low-cost behavior system. Along with detailed assembly and operation instructions, we provide data acquisition software and a robust, parallel analysis pipeline. We validate our approach by analyzing stimulus response profiles in larval zebrafish, confirming prepulse inhibition phenotypes of two previously isolated mutants, and highlighting best practices for growing larvae prior to behavioral testing. Our new design thus allows rapid construction and streamlined operation of many large-scale behavioral setups with minimal resources and fabrication expertise, with broad applications to other aquatic organisms.

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Section: Discussionmentioning

confidence: 99%

A Customizable Low-Cost System for Massively Parallel Zebrafish Behavior Phenotyping

Joo

Vivian

Graham

et al. 2020

Preprint

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“…We are slowly grasping the importance of understanding these functional units of different species and how these can aid in our understanding of disease and pathological processes [1]. While most of the research has been conducted on mammalian species, we have gained a good deal of knowledge in development and disease from other vertebrate species, with certain species of fish being utilized more and more over the last few years [2].…”

Section: Introductionmentioning

confidence: 99%

Antibodies, Nanobodies, or Aptamers—Which Is Best for Deciphering the Proteomes of Non-Model Species?

Dhar

Samarasinghe

Shigdar

2020

IJMS

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This planet is home to countless species, some more well-known than the others. While we have developed many techniques to be able to interrogate some of the “omics”, proteomics is becoming recognized as a very important part of the puzzle, given how important the protein is as a functional part of the cell. Within human health, the proteome is fairly well-established, with numerous reagents being available to decipher cellular pathways. Recent research advancements have assisted in characterizing the proteomes of some model (non-human) species, however, in many other species, we are only just touching the surface. This review considers three main reagent classes—antibodies, aptamers, and nanobodies—as a means of continuing to investigate the proteomes of non-model species without the complications of understanding the full protein signature of a species. Considerations of ease of production, potential applications, and the necessity for producing a new reagent depending on homology are presented.

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“…Danio rerio (Hamilton, 1822), commonly known as zebrafish, are widely used as vertebrate models for prediction of pollutants (neuro)toxicity in aquatic systems due to their complex behavioral responses, short breeding cycle, transparent embryos, and high similarity with human genome [ 51 , 52 ]. Taken together, the aim of this study was to evaluate the possible protective role of vit.…”

Section: Introductionmentioning

confidence: 99%

Vitamin C Attenuates Oxidative Stress and Behavioral Abnormalities Triggered by Fipronil and Pyriproxyfen Insecticide Chronic Exposure on Zebrafish Juvenile

et al. 2020

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Chronic exposure to synthetic insecticides in the early life of a child can lead to a series of disorders. Several causes as parental age, maternal smoking, birth complications, and exposure to toxins such as insecticides on childhood can lead to Autism spectrum disorder (ASD) occurrence. The aim of this study was to evaluate the potential protective role of vitamin C (Vit. C) from children’s supplements after 14 days chronic exposure to insecticide mixture fipronil (Fip) + pyriproxyfen (Pyr) on juvenile zebrafish for swimming performances, social behavior and oxidative stress associated with ASD model. Juvenile (14–17 mm) wild-type AB zebrafish (Danio rerio) (45 days) were exposed to relevant concentrations: vit. C (25 µg L−1), Fip (600 µg L−1/1.372 μM) + Pyr (600 µg L−1/1.89 μM), and [Fip (600 µg L−1/1.372 μM) + Pyr (600 µg L−1 /1.89 μM)] + vit. C (25 µg L−1). Our results showed that insecticides can disturb the social behavior of zebrafish during 14 days of the administration, decreased the swimming performances, and elevated the oxidative stress biomarkers of SOD (superoxide dismutase), GPx (glutathione peroxidase), and MDA (malondialdehyde). The vitamin C supplement significantly attenuated the neurotoxicity of insecticide mixture and oxidative stress. This study provides possible in vivo evidence to show that vitamin C supplements could attenuate oxidative stress and brain damage of fipronil and pyriproxyfen insecticide chronic exposure on zebrafish juvenile.

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The use of zebrafish (Danio rerio) as biomedical models

Cited by 166 publications

References 48 publications

A Customizable Low-Cost System for Massively Parallel Zebrafish Behavior Phenotyping

A Customizable Low-Cost System for Massively Parallel Zebrafish Behavior Phenotyping

Antibodies, Nanobodies, or Aptamers—Which Is Best for Deciphering the Proteomes of Non-Model Species?

Vitamin C Attenuates Oxidative Stress and Behavioral Abnormalities Triggered by Fipronil and Pyriproxyfen Insecticide Chronic Exposure on Zebrafish Juvenile

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