The use of wound healing assessment methods in psychological studies: A review and recommendations

Koschwanez, Heidi E.; Broadbent, Elizabeth

doi:10.1348/135910710x524633

Cited by 17 publications

(14 citation statements)

References 154 publications

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“…To mimic this mechanically induced barrier disruption (Koschwanez and Broadbent) (Taljebini et al , 1996), we performed tape-stripping in human discarded skins. TJ barrier recovery was monitored by measuring TEER and the paracellular fluorescein flux using the modified micro-Snapwell™ system with and without Pam3CSK4 treatment (see Methods and Figure 4a).…”

Section: Resultsmentioning

confidence: 99%

Activation of Epidermal Toll-Like Receptor 2 Enhances Tight Junction Function: Implications for Atopic Dermatitis and Skin Barrier Repair

Kuo

Carpenter-Mendini

Yoshida

et al. 2013

Journal of Investigative Dermatology

142

136

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Atopic dermatitis (AD) is characterized by epidermal tight junction (TJ) defects and a propensity for Staphylococcus aureus (S. aureus) skin infections. S. aureus is sensed by many pattern recognition receptors including toll-like receptor (TLR) 2. We hypothesized that an effective innate immune response will include skin barrier repair and that this response is impaired in AD subjects. S. aureus-derived peptidoglycan (PGN) and synthetic TLR2 agonists enhanced TJ barrier and increased expression of TJ proteins, CLDN1, CLDN23, occludin and ZO-1 in primary human keratinocytes. A TLR2 agonist enhanced skin barrier recovery in human epidermis wounded by tape-stripping. Tlr2−/− mice had a delayed and incomplete barrier recovery following tape-stripping. AD subjects had reduced epidermal TLR2 expression as compared to nonatopic (NA) subjects, which inversely correlated (r= 0.654, P= 0.0004) with transepidermal water loss (TEWL). These observations indicate that TLR2 activation enhances skin barrier in murine and human skin and is an important part of a wound repair response. Reduced epidermal TLR2 expression observed in AD patients may play a role in their incompetent skin barrier.

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Section: Resultsmentioning

confidence: 99%

Activation of Epidermal Toll-Like Receptor 2 Enhances Tight Junction Function: Implications for Atopic Dermatitis and Skin Barrier Repair

Kuo

Carpenter-Mendini

Yoshida

et al. 2013

Journal of Investigative Dermatology

142

136

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“…Wound healing is a biological restorative response to tissue injury that undergoes hemostasis, inflammation, repair, and remodeling through interactions between cells, extracellular matrix (ECM), and growth factors. 1,2 ECM degradation is one of the essential steps during healing as it facilitates removal of damaged tissue and provisional matrix for vessels formation and cell migration ahead. 3 Various extracellular proteinases, belonging to the serine proteinase and matrix metalloproteinase (MMP) families degrade variety of substrates including ECM.…”

Section: Introductionmentioning

confidence: 99%

Sericin–carboxymethyl cellulose porous matrices as cellular wound dressing material

Nayak

Kundu

2013

J Biomedical Materials Res

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In this study, porous three-dimensional (3D) hydrogel matrices are fabricated composed of silk cocoon protein sericin of non-mulberry silkworm Antheraea mylitta and carboxymethyl cellulose. The matrices are prepared via freeze-drying technique followed by dual cross-linking with glutaraldehyde and aluminum chloride. The microstructure of the hydrogel matrices is assessed using scanning electron microscopy and biophysical characterization are carried out using Fourier transform infrared spectroscopy and X-ray diffraction. The transforming growth factor β1 release from the cross-linked matrices as a growth factor is evaluated by immunosorbent assay. Live dead assay and 3-[4,5-dimethylthiazolyl-2]-2,5-diphenyl tetrazolium bromide assay show no cytotoxicity of blended matrices toward human keratinocytes. The matrices support the cell attachment and proliferation of human keratinocytes as observed through scanning electron microscope and confocal images. Gelatin zymography demonstrates the low levels of matrix metalloproteinase 2 (MMP-2) and insignificant amount of MMP-9 in the culture media of cell seeded matrices. Low inflammatory response of the matrices is indicated through tumor necrosis factor alpha release assay. The results indicate that the fabricated matrices constitute 3D cell-interactive environment for tissue engineering applications and its potential use as a future cellular biological wound dressing material.

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“…Wound healing involves complex cellular and molecular interactions that are influenced not only by physical health but also by psychological state [1]. For example, restraint stress has been reported to delay dermal wound healing in mice [2].…”

Section: Introductionmentioning

confidence: 99%

Social Isolation Impairs Oral Palatal Wound Healing in Sprague-Dawley Rats: A Role for miR-29 and miR-203 via VEGF Suppression

Yang¹,

Engeland

Cheng³

2013

PLoS ONE

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ObjectiveTo investigate the effects of social isolation on oral mucosal healing in rats, and to determine if wound-associated genes and microRNAs (miRNAs) may contribute to this response.MethodsRats were group housed or socially isolated for 4 weeks before a 3.5 mm wound was placed on the hard oral palate. Wound closure was assessed daily and tissues were collected for determination of gene expression levels and miRNAs (i.e., miR-29a,b,c and miR-203). The predicted target of these microRNAs (i.e., vascular endothelial growth factor A, VEGFA) was functionally validated.ResultsSocial isolation stress delayed the healing process of oral palatal mucosal wounds in rats. Lower mRNA levels of interleukin-1β (IL1β), macrophage inflammatory p r o t e i n-1α (MIP1α), fibroblast growth factor 7 (FGF7), and VEGFA were found in the biopsied tissues of isolated animals on days 1 and/or 3 post-wounding. Intriguingly, the isolated rats persistently exhibited higher levels of miR-29 family members and miR-203. Our results confirmed that VEGFA is a direct target of these miRNAs, as both miR-29a,c and miR-203 strongly and specifically suppressed endogenous VEGFA expression in vitro.ConclusionsThis study in rats demonstrates for the first time that social isolation delays oral mucosal healing, and suggests a potential role for healing-associated gene and miRNA interactions during this process via modulation of VEGF expression.

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The use of wound healing assessment methods in psychological studies: A review and recommendations

Cited by 17 publications

References 154 publications

Activation of Epidermal Toll-Like Receptor 2 Enhances Tight Junction Function: Implications for Atopic Dermatitis and Skin Barrier Repair

Activation of Epidermal Toll-Like Receptor 2 Enhances Tight Junction Function: Implications for Atopic Dermatitis and Skin Barrier Repair

Sericin–carboxymethyl cellulose porous matrices as cellular wound dressing material

Social Isolation Impairs Oral Palatal Wound Healing in Sprague-Dawley Rats: A Role for miR-29 and miR-203 via VEGF Suppression

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