The Use of Viable Frozen Lymphocytes for Studies in Human Tumor Immunology

Golub, Sidney H.; Sulit, Hector L.; Morton, Donald L.

doi:10.1097/00007890-197503000-00001

Cited by 56 publications

(18 citation statements)

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“…Careful studies of the non-T-cell subpopulations are needed to investigate this point. A smaller decrease in cytotoxic activity has been observed by Golub et al (1975). The results here are similar to our results in the rat (Ortaldo et al, 1976).…”

Section: Discussionsupporting

confidence: 93%

Cryopreservation of human lymphocyte function as measured by in vitro assays

Oldham

Dean²,

Cannon³

et al. 1976

Intl Journal of Cancer

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Methods are described by which cryopreserved cells can be utilized in a number of in vitro assays. On a per cell basis, nearly total recovery of function can be demonstrated for lymphocyte transformation (mitogens, antigens and MLC) and rosette-forming cells. Excellent recovery of mononuclear cell production of leukocyte migration inhibitory factor was also observed. Highly reproducible activity was recovered on a per lymphocyte basis in lymphocyte cytotoxicity but with a definite decrement in the percentage recovery. Both for longitudinal studies of immune function and for standardization of these assays in one or more laboratories such cryopreserved cells are ofimmense value and should be widely utilized.

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Section: Discussionsupporting

confidence: 93%

Cryopreservation of human lymphocyte function as measured by in vitro assays

Oldham

Dean²,

Cannon³

et al. 1976

Intl Journal of Cancer

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“…The method for isolat ing. processing, and storing human lymphoid cells has been previously reported (8). Peripheral blood lym phocytes (PBL) were obtained from normal donors aged 21-38.…”

Section: Methodsmentioning

confidence: 99%

“…The induction of lymphocyte blastogenesis by mitogens or by allo geneic cells has been described [8]. Briefly, 10' lym phocytes were incubated in complete medium consist ing of RPMI-1640 (Microbiological Associates, Inc., Bethesda, Md.…”

Section: Methodsmentioning

confidence: 99%

Augmentation of Human Thymocyte Proliferative Responses by Retinoic Acid

Sidell¹,

Famatiga²,

Golub³

1981

Pathobiology

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When 10^–5 to 10^-7M all-trans retinoic acid (RA) was added to human thymocyte or tonsil lymphocyte cultures in the presence of mitogens or allogeneic stimulator cells, blastogenesis was increased up to 2.5-fold. No augmentation in proliferative responses of peripheral blood or spleen lymphocytes was observed. In the thymus, lymphocytes in the subclass of cells that did not bind peanut agglutinin (PNA) were responsible for the RA-induced enhancement. RA also increased the number of mitogen-stimulated thymocyte colonies developing in soft agar. These results indicate that the targets of RA activity must be lymphoid cells at a later stage of maturation than those identified by binding to PNA, and that one mechanism of RA enhancement is an increase in the number of lymphocytes that undergo blast transformation.

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“…Peripheral blood lymphoid cells were obtained from the patients prior to treatment and at 15, 30, 60, and 90 days after the initiation of therapy and were cryopreserved as previously described [4]; all in vitro assays were performed on all samples from a single patient on 1 day.…”

Section: In Vitro Assaysmentioning

confidence: 99%