Cryopreservation of human lymphocyte function as measured by in vitro assays

Abstract

Methods are described by which cryopreserved cells can be utilized in a number of in vitro assays. On a per cell basis, nearly total recovery of function can be demonstrated for lymphocyte transformation (mitogens, antigens and MLC) and rosette-forming cells. Excellent recovery of mononuclear cell production of leukocyte migration inhibitory factor was also observed. Highly reproducible activity was recovered on a per lymphocyte basis in lymphocyte cytotoxicity but with a definite decrement in the percentage recovery. Both for longitudinal studies of immune function and for standardization of these assays in one or more laboratories such cryopreserved cells are ofimmense value and should be widely utilized.

Repetitive Testing and Storage of Human Effector Cells in Natural Killing and Antibody-Dependent Cell-Mediated Cytotoxicity

“…For cryopreservation, we used a freezer core, the height of which was adjusted to de crease the temperature of the cell suspensions approx imately -2°C/min. Our results are comparable to those of 3 previous investigations with respect to per cent recovery of PBM, viability and percent recovery of cytotoxicity in ADCC or NK assays [Oldham et al, 1976;Callery et al, 1980;Karpovitch et al, 1980]. Therefore the freezer core appears to be as effective as a programmed freezer for this purpose.…”

Immunomodulatory Effects of Iscador: A <i>Viscum album</i> Preparation

“…I larvesling was performed using Tilertck Cell Harves ter (Flow Laboratories AG) and beta radiation was measured in a liquid scintillation counter. Parallel with count per minute (cpm) values stimulation index (SI) was also calculated from the formula: c| cpm in culture stimulated by mitogen cpm in control culture l or standardization of these assays cryoprcservcd peripheral lymphocytes obtained from I donor by a technique recommended by Oldham et al [60] were used.…”

Slow versus Fast Postthaw Dilution for the Recovery of Lymphocytes in Whole Blood Frozen in either Dimethyl Sulfoxide or Glycerol

“…Smith (1950) first emphasized osmotic effects during the postthaw recovery of human red blood cells. Since then, variations of a slow dilution technique have been used in leukocyte preservations (Atkins, 1962;Goodman, 1963;Ashwood-Smith, 1964;Pegg, 1965;Cavins et al, 1968;Chess et al, 1972;Oldham et al, 1976). Fast dilution techniques also have been used for the cryoprotection of leukocytes (Brody et al, 1968;Thomson and O 'Connor, 1971;Knight et al, 1972;Weed et al, 1972;Farrant et al, 1974;Strong et al, \914\Birkeland, 1976;Weiner, 1976).…”