The use of the tetrazolium reduction test for the detection of the terminal electron transport system (ETS) activity in decomposing reed (Phragmites australis /Cav./ Trin. ex Steud.) rhizome

Szabó, Edit

doi:10.1051/limn/2003006

Cited by 8 publications

(4 citation statements)

References 28 publications

(42 reference statements)

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“…From days 292-547 all of the examined microbiological parameters decreased. The variation of the ETS activity during decomposition showed similar trends as in other studies (Maamri et al 1998;Szabó 2003).…”

Section: Microbiological Parameterssupporting

confidence: 88%

“…The electron transport system (ETS) assay described by Kenner & Ahmed (1975) was used to determine the potential microbial respiratory activity, after adaptation of the method to decomposing plant material (Szabó 2003). 20-40 mg subsamples of fresh plant litter were homogenized in icecold buffer consisting of MgSO4 (75 µM), Triton-X-100 0.5% (v/v), polyvinyl pyrolidone (PVP) 1.5% w/v, in 0.1 M phosphate buffer (pH 8.4) for 5 minutes at 0-4 • C, by using a potter homogenizer (5 000 rpm, 0-4 • C, B. Braun Biotech.…”

Section: Introductionmentioning

confidence: 99%

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Decomposition of Typha angustifolia and Phragmites australis in the littoral zone of a shallow lake

Ágoston-Szabó

Dinka

2008

Biologia

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Decomposition of air-dried live Typha angustifolia (L) stems and leaves and Phragmites australis (Cav. Trin ex Steud.) leaves and culms were studied in a shallow freshwater lake (Lake Fehér, Fertő-Hanság National Park, Hungary) using the litter bag technique. Samples were analyzed for dry mass, fiber (cellulose, hemicelluloses, lignin) and nutrient (C, N, P, S) contents, litter-associated fungal biomass (ergosterol concentration), potential microbial respiration (electron transport activity: ETS) and cellulolytic bacteria. In terms of mesh size, there were no significant differences in the examined parameters of P. australis leaves and culms and T. angustifolia stems with leaves. P. australis leaves had the highest rate of decomposition and P. australis culms the lowest. Hemicellulose degraded more rapidly than the other fibers, while the lignin had the slowest rate of decomposition. The ETS activity of the examined plant litter types increased from day 91 st to 237 th while decomposition processes were most active, ergosterol contents were high, and there were few cellulolytic bacteria. The counts of cellulolytic bacteria fluctuated during the decomposition period, they were high at the beginning then they decreased. In each case bacteria were found to be the first colonizers of plant detritus, and were followed by fungal growth.

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Section: Microbiological Parameterssupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Decomposition of Typha angustifolia and Phragmites australis in the littoral zone of a shallow lake

Ágoston-Szabó

Dinka

2008

Biologia

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“…The method which was introduced by PACKARD (1971) has been widely used and modified since then (KENNER and AHMED, 1975;ZIMMER-MAN, 1975;CHRISTENSEN, 1983;RELEXANS, 1996;SIMČIČ and BRANCELJ, 2002). ETS-activity is influenced by many factors, temperature being the most evident (SIMČIČ and BRANCELJ, 1997;SZABO, 2003). The significant increase of respiratory potential usually occurs after some days of exposure of organisms to higher temperatures (SIMČIČ and BRANCELJ, 1997) but no relation to seasonal temperature changes was shown in our research.…”

Section: Discussioncontrasting

confidence: 52%

The Relationship of the Processes in the Rhizosphere of Common Reed Phragmites australis, (Cav.) Trin. ex Steudel to Water Fluctuation

Urbanc‐Berćić¹,

Gaberščik²

2004

International Review of Hydrobiology

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The Relationship of the Processes in the Rhizosphere of CommonReed Phragmites australis, (CAV.) TRIN. ex STEUDEL to Water Fluctuation key words: water level fluctuation, reed stand, ETS-activity, inorganic nutrients, soil water. AbstractIntermittent Lake Cerknica is characterised by extreme water level fluctuations. It is neither a lake, nor a terrestrial ecosystem. The decomposition processes in the rhizosphere of extended reed stands are essential for the entire ecosystem. The influence of intermittent water regime on the microbial activity and availability of nutrients in the rhizosphere in a terrestrial reed stand was investigated. The measurements revealed a relatively high soil water content, that was not related to underground water table. Mineralization processes in a soil profile were estimated by measuring microbial activity as electron transport system (ETS) activity and by nutrients in soil water. The microbial activity was determined in the whole depth profile. The highest ETS activity of a soil was 0. . The values were not related to underground water table. All forms of nutrients were constantly present, showing no correlation with the soil water content. ANOVA revealed significant decrease of nitrate with depth, but not for nitrite, ammonium and ortho-phosphate. IntroductionThe intermittent Lake Cerknica is characterised by extreme water level fluctuations. A part of the year the area is flooded, but from time to time, usually in summer, it becomes dry. The lake is the locus typicus for intermittent lakes and poljes (karst fields). Wetting and drying occurs seasonally, depending on precipitation. The exchanging of dry and wet periods influences the dynamics of biota and the process of mineralisation (URBANC-BERČIČ and GABERŠČIK, 2001). Common reed (Phragmites australis) is a dominant wetland species in the area. With respect to the water regime three types of reed stands were distinguished: terrestrial, land-water interface, and littoral stands. Stands exhibited significant differences in primary productivity parameters (GABERŠČIK et al., 2000), in the composition and depth of the soil layer, and in the variations of water regime (URBANC-BERČIČ and GABERŠČIK, 2001; URBANC-BERČIČ and GABERŠČIK, in press). Variations of water regime are fundamental for the nutrient cycling in the sediment (BROCK, 1986;BOULTON and BROCK, 1999). Most chemical features depending on aerobic and anaerobic conditions are highly variable. A presence of oxygen in a soil increases the microbial activity and the mineralization rate at the beginning of drying. Later on drying can cause considerable impact on sediment properties by affecting mineral phases and by killing up to 76% of the microbial biomass (QUI and MCCOMB, 1995). These changes reduce the capacity of the sediment to adsorb nutrients, such Internat. Rev. Hydrobiol.

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“…ETS activity indicates the amount of oxygen consumption that would occur if all enzymes functioned at their maximum activity (Muskó et al, 1995). The ETS assay has proved to be a good tool for estimating the potential metabolic activity of zooplankton (Owens & King, 1975;James, 1987;Simčič & Brancelj, 1997;Hernández-León et al, 2001;Simčič & Brancelj, 2009;Osma et al, 2016), microplankton (Kenner & Ahmed, 1975b;Devol & Packard, 1978;Packard, 1985;Simčič & Germ, 2009;Packard et al, 2015), macrozoobenthos (Muskó et al, 1995;Simčič, 2005;Simčič & Germ, 2009;Moreira et al, 2017), macrophytes (Peñuelas et al, 1988;Szabó, 2003;Simčič & Germ, 2009;Germ & Simčič, 2011) and microorganisms in sediments (G. -Tóth et al, 1994;Simčič & Brancelj, 2002;de Vicente et al, 2006;Simčič & Brancelj, 2009;Germ & Simčič, 2011;Krausz et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Respiration rates in shallow lakes of different types: contribution of benthic microorganisms, macrophytes, plankton and macrozoobenthos

et al. 2018

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The determination of the metabolic activity of organisms at various trophic levels is crucial for the proper assessment of the energy flow through the ecosystem, which is the basic process determining ecosystem functioning. We estimated the respiration rate in nine shallow, eutrophic lakes (macrophytedominated and phytoplankton-dominated) from northeastern Poland. Respiratory carbon loss (RCL) through bottom microbial communities, macrophytes, plankton and macrozoobenthos was estimated by measuring the Electron Transport System activity. The shares of the particular ecosystem components in respiration processes differed among the lake types and seasons. The bottom microbial communities contributed most to the RCL (from 50% in the macrophyte-dominated lakes to 90% in the shallower phytoplankton-dominated lakes) except in macrophytes-dominated lakes in summer, where the macrophyte contribution prevailed (80%). The contribution of plankton was considerable only in the deeper phytoplankton-dominated lakes (20%). Macrozoobenthos was important (20%) only in the macrophytedominated lakes in spring and autumn. The RCL through bottom microbial communities was substantially higher in the shallow lakes (especially phytoplankton-dominated) than in deep, stratified eutrophic lakes. Shallow eutrophic lakes can be highly productive due to intensive organic matter mineralization at the bottom and rapid flow and cycling of carbon and nutrients resulting from their polymictic character.

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The use of the tetrazolium reduction test for the detection of the terminal electron transport system (ETS) activity in decomposing reed (Phragmites australis /Cav./ Trin. ex Steud.) rhizome

Cited by 8 publications

References 28 publications

Decomposition of Typha angustifolia and Phragmites australis in the littoral zone of a shallow lake

Decomposition of Typha angustifolia and Phragmites australis in the littoral zone of a shallow lake

The Relationship of the Processes in the Rhizosphere of Common Reed Phragmites australis, (Cav.) Trin. ex Steudel to Water Fluctuation

Respiration rates in shallow lakes of different types: contribution of benthic microorganisms, macrophytes, plankton and macrozoobenthos

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