The use of the dynamic weight bearing test to assess the effects of acute, intramuscularly administered botulinum neurotoxin type A1 in rats

Cornet, Sylvie; Périer, Cindy; Wagner, Stéphanie; Andriambeloson, Emile; Pouzet, Bruno; Kalinichev, Mikhail

doi:10.1016/j.toxcx.2020.100041

Cited by 3 publications

(5 citation statements)

References 42 publications

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“…CMAP amplitude has a high sensitivity to rBoNT/A1 activity. 6 , 31 , 33 Specifically, inhibition of CMAP amplitude was obtained at a very low rBoNT/A1 dose (0.50 pg/kg) and lasted at least 30 days. Conversely, in the DAS assay, at the dose approaching ED 50 (4.3 pg/kg), the effect lasted between 8 and 10 days.…”

Section: Discussionmentioning

confidence: 95%

“…rBoNT/A1 injection into the gastrocnemius lateralis muscle. CMAP amplitude has a high sensitivity to rBoNT/A1 activity 6,31,33 . Specifically, inhibition of CMAP amplitude was obtained at a very low rBoNT/A1 dose (0.50 pg/kg) and lasted at least 30 days.…”

Section: Discussionmentioning

confidence: 98%

“…CMAP assessment was performed as previously described 6 and recording was performed with a Keypoint electromyograph (Medtronic). Subcutaneous monopolar needle electrodes (No 9013R0312; Medtronic) were used for both stimulation and recording.…”

Section: Methodsmentioning

confidence: 99%

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Recombinant botulinum neurotoxin serotype A1 in vivo characterization

Périer

Martin

Cornet

et al. 2021

Pharmacology Res & Perspec

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Clinically used botulinum neurotoxins (BoNTs) are natural products of Clostridium botulinum. A novel, recombinant BoNT type A1 (rBoNT/A1; IPN10260) has been synthesized using the native amino acid sequence expressed in Escherichia coli and has previously been characterized in vitro and ex vivo. Here, we aimed to characterize rBoNT/A1 in vivo and evaluate its effects on skeletal muscle. The properties of rBoNT/A1 following single, intramuscular administration were evaluated in the mouse and rat digit abduction score (DAS) assays and compared with those of natural BoNT/A1 (nBoNT/A1). rBoNT/A1‐injected tibialis anterior was assessed in the in situ muscle force test in rats. rBoNT/A1‐injected gastrocnemius lateralis (GL) muscle was assessed in the compound muscle action potential (CMAP) test in rats. The rBoNT/A1‐injected GL muscle was evaluated for muscle weight, volume, myofiber composition and immunohistochemical detection of cleaved SNAP25 (c‐SNAP25). Results showed that rBoNT/A1 and nBoNT/A1 were equipotent and had similar onset and duration of action in both mouse and rat DAS assays. rBoNT/A1 caused a dose‐dependent inhibition of muscle force and a rapid long‐lasting reduction in CMAP amplitude that lasted for at least 30 days. Dose‐dependent reductions in GL weight and volume and increases in myofiber atrophy were accompanied by immunohistochemical detection of c‐SNAP25. Overall, rBoNT/A1 and nBoNT/A1 exhibited similar properties following intramuscular administration. rBoNT/A1 inhibited motoneurons neurotransmitter release, which was robust, long‐lasting, and accompanied by cleavage of SNAP25. rBoNT/A1 is a useful tool molecule for comparison with current natural and future modified recombinant neurotoxins products.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 98%

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Recombinant botulinum neurotoxin serotype A1 in vivo characterization

Périer

Martin

Cornet

et al. 2021

Pharmacology Res & Perspec

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“…Two separate measures of pain-related behavior were assessed pre-surgery and at 1 through 6 weeks after MMx surgery using rats for analysis of NGF: changes in dynamic weight-bearing distribution and mechanical sensitivity of the hind paw 16,21,22 . Imbalance of dynamic weight distribution was measured using a dynamic weight bearing test 2.0 ® (BioSeb; Vitrolles, France) including a Plexiglas cage, a floor equipped with capture to detect pressure variations and a camera positioned above the cage to record and validate the animal's posture during analysis.…”

Section: Pain-related Behaviormentioning

confidence: 99%

Time course and localization of nerve growth factor expression and sensory nerve growth during progression of knee osteoarthritis in rats

Aso¹,

Walsh²,

Wada³

et al. 2022

Osteoarthritis and Cartilage

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“…C57BL/6 mice (aged 5–6 weeks) were randomly divided into the WT group (control group), Skin Pcluster group, Botox 0.004 U group, and Botox 0.008 U group, with no less than 3 mice in each group. The method of administration was intramuscular injection in the gastrocnemius muscle of mice with a dose of 20 μg per mouse ( Cornet et al, 2020a ; Cornet et al, 2020b ). The images of the morphology were recorded by camera at 1 h and 2 h after injection.…”

Section: Methodsmentioning

confidence: 99%

A nanohybrid synthesized by polymeric assembling Au(I)-peptide precursor for anti-wrinkle function

Liú

Huang

Mao

et al. 2022

Front. Bioeng. Biotechnol.

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A major sign of aging is wrinkles (dynamic lines and static lines) on the surface of the skin. In spite of Botulinum toxin’s favorable therapeutic effect today, there have been several reports of its toxicity and side effects. Therefore, the development of an effective and safe wrinkle-fighting compound is imperative. An antioxidant-wrinkle effect was demonstrated by the peptide that we developed and synthesized, termed Skin Peptide. Aiming at the intrinsic defects of the peptide such as hydrolysis and poor membrane penetration, we developed a general approach to transform the Skin Peptide targeting intracellular protein-protein interaction into a bioavailable peptide-gold spherical nano-hybrid, Skin Pcluster. As expected, the results revealed that Skin Pcluster reduced the content of acetylcholine released by neurons in vitro, and then inhibit neuromuscular signal transmission. Additionally, human experiments demonstrated a significant de-wrinkle effect. Moreover, Skin Pcluster is characterized by a reliable safety profile. Consequently, anti-wrinkle peptides and Skin Pcluster nanohybrids demonstrated innovative anti-wrinkle treatments and have significant potential applications.

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The use of the dynamic weight bearing test to assess the effects of acute, intramuscularly administered botulinum neurotoxin type A1 in rats

Cited by 3 publications

References 42 publications

Recombinant botulinum neurotoxin serotype A1 in vivo characterization

Recombinant botulinum neurotoxin serotype A1 in vivo characterization

Time course and localization of nerve growth factor expression and sensory nerve growth during progression of knee osteoarthritis in rats

A nanohybrid synthesized by polymeric assembling Au(I)-peptide precursor for anti-wrinkle function

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