Time course and localization of nerve growth factor expression and sensory nerve growth during progression of knee osteoarthritis in rats

Aso, Koji; Walsh, David A.; Wada, Hiroyuki; Izumi, Masashi; Tomitori, H.; Fujii, K.; Ikeuchi, Masahiko

doi:10.1016/j.joca.2022.07.003

Cited by 10 publications

(19 citation statements)

References 39 publications

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“…The follow-up periods were not very long, but 8 weeks would be sufficient because bone mineral density started to decrease at 2 weeks 49 and further decreased at 4 and 8 weeks in the ovariectomized rat OP model 50 . We confirmed that severe cartilage degeneration occurred within 6 weeks with full thickness involvement in the tidemark in a medial meniscectomy-induced rat OA model 51 . Studies performing assessments at multiple time points are needed in the future.…”

Section: Discussionsupporting

confidence: 76%

The effect of postmenopausal osteoporosis on subchondral bone pathology in a rat model of knee osteoarthritis

Wada

Aso

Izumi

et al. 2023

Sci Rep

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This study aimed to investigate the additional effect of ovariectomy-induced osteoporosis (OP) on the pathology of knee osteoarthritis (OA) in a rat meniscectomized model, particularly focusing on subchondral bone changes and pain behaviour. Rats were divided into four groups, sham, OP, OA, OP plus OA, and assessed for histology, osteoclast activity, subchondral bone microstructure, and pain-related behaviour. Rats with OP plus OA had significantly increased calcified cartilage and subchondral bone damage scores, increased densities of subchondral osteoclasts in the weight-bearing area, and more porous subchondral trabecular bone compared with rats with OA. Loss of tidemark integrity was observed most frequently in rats with OP plus OA. The density of subchondral osteoclasts correlated with the calcified cartilage and subchondral bone damage score in rats with OA (OA and OP plus OA). No significant differences in the receptor activator of nuclear factor-kappa B ligand (RANKL)/osteoprotegerin (OPG) expression ratio in subchondral bone and pain-related behavioural tests were observed between rats with OA and rats with OP plus OA. In rats with OA, coexisting OP potentially aggravated OA pathology mainly in calcified cartilage and subchondral trabecular bone by increasing subchondral osteoclast activity.

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Section: Discussionsupporting

confidence: 76%

The effect of postmenopausal osteoporosis on subchondral bone pathology in a rat model of knee osteoarthritis

Wada

Aso

Izumi

et al. 2023

Sci Rep

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“…Nerve growth factor concentration has been shown to be increased in synovial fluid and chondrocytes from joints of horses with naturally‐occurring osteoarthritis 19 . In a rat model of osteoarthritis, NGF increased in synovial membranes, chondrocytes, and subchondral bone after medial meniscectomy 20 . Nerve growth factor can cause neuronal sensitization, 21 it increases nerve growth and sprouting, 22 and NGF transcription has been found to be increased in the dorsal horn of rats 8 weeks after destabilization of the medial meniscus 23 .…”

Section: Discussionmentioning

confidence: 99%

“… 19 In a rat model of osteoarthritis, NGF increased in synovial membranes, chondrocytes, and subchondral bone after medial meniscectomy. 20 Nerve growth factor can cause neuronal sensitization, 21 it increases nerve growth and sprouting, 22 and NGF transcription has been found to be increased in the dorsal horn of rats 8 weeks after destabilization of the medial meniscus. 23 These findings indicate that NGF plays an active role in osteoarthritis‐associated pain both via local processes within the joint and in centralized pain adaptions in the dorsal horn of the spinal cord.…”

Section: Discussionmentioning

confidence: 99%

Serum nerve growth factor in horses with osteoarthritis‐associated lameness

Kendall

Lützelschwab

Lundblad

et al. 2023

Veterinary Internal Medicne

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Background: Nerve growth factor (NGF) is a neurotrophin that is increased in osteoarthritic joints of horses. In humans, NGF has been associated with pain, and both synovial and serum NGF concentrations are increased in osteoarthritic patients. Studies in humans also have shown that serum NGF concentration can increase with stress. Serum NGF concentration should be evaluated in horses with osteoarthritis-associated lameness.Objectives: Quantify and compare serum NGF concentration in horses with osteoarthritis-associated lameness and sound horses. Additionally, the impact of short-term stress on serum NGF concentration was investigated.Animals: Lame horses with radiographic evidence of osteoarthritis (n = 20), lame horses without radiographic changes in the affected joint (n = 20) and sound horses (n = 20). In addition, horses with acute fractures (n = 9) were sampled. To determine the effect of stress, serum from horses subjected to a stressful event (transportation, n = 5; stress confirmed by increased serum cortisol concentration) was analyzed.Methods: Cross-sectional clinical study (lame, sound, and fracture cohorts) and experimental longitudinal study (stress cohort). Serum NGF concentration was determined using a quantitative sandwich ELISA.Results: Serum NGF concentration was increased in lame horses with radiographic evidence of osteoarthritis (P < .0001; median, 238 pg/mL; interquartile range [IQR], 63-945 pg/mL) and in lame horses without radiographic evidence of osteoarthritis in the painful joint (P < .05; median, 31 pg/mL; IQR, 31-95 pg/mL) compared with sound horses (median, 31 pg/mL; IQR, 31-46 pg/mL). Serum NGF concentration did not increase with short-term stress and was low in horses with fractureassociated pain.Conclusions and Clinical Importance: Serum NGF concentration was high in the cohort with advanced osteoarthritis and should be investigated as a marker for osteoarthritis-associated pain.

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“… 175 The colocalization of nerve growth factor with an increased density of nerve fibers in joint degeneration has been identified as a contributing factor to the sensation of arthritis pain by sensitizing the peripheral nerve endings. 176 Therefore, it is supposed that estrogen plays important roles in angiogenesis and neurogenesis against FJOA via angiogenic cytokines, which needs further verification. In addition, cartilage is one of the major elements in joint structure.…”

Section: Molecular Mechanisms By Which Estrogen Alleviates Ivd Degene...mentioning

confidence: 99%

Low back pain and osteoarthritis pain: a perspective of estrogen

et al. 2023

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Low back pain (LBP) is the world’s leading cause of disability and is increasing in prevalence more rapidly than any other pain condition. Intervertebral disc (IVD) degeneration and facet joint osteoarthritis (FJOA) are two common causes of LBP, and both occur more frequently in elderly women than in other populations. Moreover, osteoarthritis (OA) and OA pain, regardless of the joint, are experienced by up to twice as many women as men, and this difference is amplified during menopause. Changes in estrogen may be an important contributor to these pain states. Receptors for estrogen have been found within IVD tissue and nearby joints, highlighting the potential roles of estrogen within and surrounding the IVDs and joints. In addition, estrogen supplementation has been shown to be effective at ameliorating IVD degeneration and OA progression, indicating its potential use as a therapeutic agent for people with LBP and OA pain. This review comprehensively examines the relationship between estrogen and these pain conditions by summarizing recent preclinical and clinical findings. The potential molecular mechanisms by which estrogen may relieve LBP associated with IVD degeneration and FJOA and OA pain are discussed.

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Time course and localization of nerve growth factor expression and sensory nerve growth during progression of knee osteoarthritis in rats

Cited by 10 publications

References 39 publications

The effect of postmenopausal osteoporosis on subchondral bone pathology in a rat model of knee osteoarthritis

The effect of postmenopausal osteoporosis on subchondral bone pathology in a rat model of knee osteoarthritis

Serum nerve growth factor in horses with osteoarthritis‐associated lameness

Low back pain and osteoarthritis pain: a perspective of estrogen

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