The use of steroid hormones in superovulation of Nelore donors at different stages of estrous cycle

Andrade, J.C.O; Oliveira, Marcos Antônio Lemos; Lima, Paulo Ricardo Silva; Guido, Sebastião Inocêncio; Bartolomeu, Cláudio Coutinho; Filho, Fernando Tenório; Pina, V.M.R; Iunes-Souza, T. C.; Paula, Ney Rômulo de Oliveira; Freitas, José Cláudio Carneiro de

doi:10.1016/s0378-4320(03)00036-8

Cited by 12 publications

(7 citation statements)

References 21 publications

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“…The results of this intensive study have demonstrated a great prospect of induced follicular wave emergence treatments combined with super-ovulatory conventions. A similar superovulation study in Nelore (beef cows) conducted in Brazil has produced comparable results ( Andrade et al., 2003 ).…”

Section: Discussionmentioning

confidence: 80%

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GnRH or estradiol benzoate combination with CIDR improves in-vivo embryo production in bovines (Bos indicus and Bos taurus) under subtropics

Mahmood

Tahir

Butt

et al. 2021

PeerJ

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Multiple Ovulation and Embryo Transfer (MOET) technology is a potential technique to upgrade livestock species’ genetics. The varied response to super-stimulatory treatments remains one of the limiting factors to this technology’s widespread use. The present study was aimed to improve the superovulation response and in-vivo embryo production by using controlled internal drug release (CIDR)-GnRH or CIDR-EB (Estradiol Benzoate) along with conventional superovulation protocol in Holstein Frisian (HF): Bos taurus; n = 42) and Crossbred (XB: Cholistani (Bos indicus) × HF; n = 28) cows. In the CIDR-GnRH/CIDR-EB treatment, CIDR was implanted in the cows after confirming the presence of a corpus luteum (CL) on the 8th day after estrus. 2 ml GnRH (Lecirelin acetate 0.0262 mg/ml) or 2 mg EB was also administered in CIDR-GnRH/CIDR-EB groups, respectively. Both groups were given super-stimulatory treatment from the 11th day after estrus (FSH in tapering doses twice a day for four consecutive days). On day 13, two doses of 2 ml prostaglandin (75 µg/ml of dextrorotatory cloprostenol) were administered (am: pm), and CIDR was removed the following day. Two artificial inseminations (AI) of the cows were performed (12 h apart) on the 15th day. No CIDR and GnRH/E.B were given in the control group, but the remaining superovulation protocol was the same. Later on, seven days after the first AI, non-surgical embryo flushing was done. The transferable embryos produced from three different superovulation protocols were then transferred into the recipient cows (n = 90) for determining their fertility. Statistical analysis revealed that the number of super-estrus follicles (SEF), multiple corpora lutea (MCL), ovulation/fertilization percentage, fertilized structures recovered (FSR), and transferable embryos (TEs) remained significantly higher (p < 0.05), and days taken for return to estrus (RTE) after embryo collection remained significantly lower (p < 0.05) in CIDR-GnRH (n = 18) and CIDR-EB (n = 15) groups as compared to the control (n = 37). The comparison between XB and HF cows revealed that the TEs production in CIDR-GnRH (XB = 5 vs HF = 13) and CIDR-EB (XB = 6 vs HF = 9) based superovulation protocols were 11.60 ± 4.08 vs 04.31 ± 0.98 and 09.33 ± 1.78 vs 05.22 ± 1.36, respectively. TEs production in XB cows (n = 5) of the CIDR-GnRH group was significantly higher (11.60 ± 4.08) than other groups. On the other hand, the days taken for RTE after embryo collection remained significantly lower (p < 0.05) in HF cows of treatment groups. However, the fertility of TEs was neither affected significantly (p > 0.05) by the superovulation protocol used nor by breed differences among donor cows. In conclusion, using CIDR-GnRH or CIDR-EB along with conventional superovulation protocol may enhance the efficiency of MOET programs in cattle. Furthermore, XB donor cows demonstrated a better performance than HF donor cows under subtropical conditions.

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Section: Discussionmentioning

confidence: 80%

“…The range of TE among the different groups was 4–6 per donor per collection. Another study on Nelore (beef) cows has produced a higher mean number of TE with CIDR-GnRH treatment without considering the estrous cycle stage ( Andrade et al., 2003 ). However, the TEs production in beef cows is generally higher as compared to dairy cows.…”

Section: Discussionmentioning

confidence: 99%

GnRH or estradiol benzoate combination with CIDR improves in-vivo embryo production in bovines (Bos indicus and Bos taurus) under subtropics

Mahmood

Tahir

Butt

et al. 2021

PeerJ

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“…However, successful embryo transfer requires oestrus synchronization between donors and recipients to facilitate the procedure and increase the rate of pregnancy. Andrade et al [8] evaluated the superovulatory response and embryo recovery from Nelore donors following treatment with a controlled internal drug-releasing device and estradiol benzoate (CIDR-B program) at different stages of the oestrous cycle and found that a combination of steroid hormones could be used prior to superovulation in Nelore donors at any stage of the oestrous cycle without affecting the efficiency of embryo-transfer programs. As the yak is somewhat aggressive and rather difficult to handle, the present study was, therefore, designed to use CIDR-B combined with FSH to induce superovulation during uncertain stages of oestrus cycle.…”

Section: Discussionmentioning

confidence: 99%

Successful embryo transfer in Tianzhu white yak using standard protocol

Xianghong

Wang

et al. 2007

SCI CHINA SER C

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The present study was carried out to investigate the efficiency of superovulation, oestrus synchronization, and embryo recovery in Tianzhu white yaks and also to confirm the pregnancy rate of black yaks, to which embryos collected from white yaks were transplanted. Forty-seven yaks were selected from different experiment groups, including 10 Tianzhu white female yaks (donor, group A) and 37 black female yaks (recipient, groups B and C). Superovulation of the donor was induced by the application procedure of CIDR-B+FSH+PG. Oestrus synchronization of recipients was induced using two methods: group B was given the same treatment as group A, except that the follicle-stimulating hormone (FSH) injection was not administered, whereas group C was injected with cloprostenol only once when corpus luteum (corpora lutea) was (were) palpated. The results showed that the oestrous rates in group A were higher (80%) than those in group B (60%) and group C (44.5%). As for the efficiency of superovulation, it was indicated that the mean numbers (+/-SD) of total corpora lutea, follicles, viable (transferable), and degenerated embryos were 4.75+/-2.19, 1.13+/-0.83, 2.50+/-1.31, and 1.38+/-0.92, respectively. The mean embryo recovery rates were 55.6%. All together, 18 viable embryos of Tianzhu white yak were obtained and 12 of them were transplanted to 10 recipients. The pregnancy rate was 50% and the delivery rate was 40%.

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“…Superovulation response is very important for this aim and it is one of the most critical stages in embryo transfer protocols. For this respect, it is very important to know about the factors effecting superovulation response and elimination of the negative situations (Andrade et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Superovulation in cows synchronized with two different progesterone+oestradiol protocols

et al. 2013

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Abstract. A total of 26 Brown Swiss cows were used to compare the synchronization and superovulatory response of follicle stimulating hormone treated cows that were synchronized with progesterone+oestradiol valerate or benzoate. Control cows (n = 8) were superstimulated with follicle stimulating hormone using twice daily injections with decreasing doses from day 10–13 after determined reference oestrus. Cows in treatment groups were received either ear implant (n = 9) containing norgestomet+oestradiol valerate or progesterone releasing intravaginal device (n = 9) containing progesterone+oestradiol benzoate, at random stage of the oestrus cycle, for 9 days. Seven days after the implant and progesterone releasing intravaginal device insertion, follicle stimulating hormone was injected as described in the control group. There was no significant difference between the groups for superovulation responses. In conclusion, both protocols synchronized the oestrus cycle in follicle stimulating hormone treated cows and, any of the protocols evaluated in this study can be used as a pretreatment for superstimulation started on the seventh day of the implant or progesterone releasing intravaginal device insertion in Brown Swiss cows.

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The use of steroid hormones in superovulation of Nelore donors at different stages of estrous cycle

Cited by 12 publications

References 21 publications

GnRH or estradiol benzoate combination with CIDR improves in-vivo embryo production in bovines (Bos indicus and Bos taurus) under subtropics

GnRH or estradiol benzoate combination with CIDR improves in-vivo embryo production in bovines (Bos indicus and Bos taurus) under subtropics

Successful embryo transfer in Tianzhu white yak using standard protocol

Superovulation in cows synchronized with two different progesterone+oestradiol protocols

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