The use of sheepshead minnow (Cyprinodon variegatus) and a benthic copepod (Tisbe battagliai) in short-term tests for estimating the chronic toxicity of industrial effluents

Hutchinson, Thomas H.; Williams, Tim D.

doi:10.1007/bf00027824

Cited by 15 publications

(9 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Copepods used for this study were collected from continuous cultures successfully maintained at Brixham Environmental Laboratory over several years [16]. The animals were cultured in 0.2‐μm‐filtered natural sea water collected from Tor Bay, Devon, United Kingdom, and maintained at 20 ± 1°C.…”

Section: Methodsmentioning

confidence: 99%

“…We have therefore begun to explore the potential value of a life‐cycle test protocol with Tisbe battagliai (Crustacea, Copepoda, Harpacticoida) for testing EDCs. This marine organism has proven to be sensitive to a range of complex mixtures and single substances [16–19], has been successfully used in a bioassay role for North Sea water quality monitoring [20], and is representative of an ecologically widespread group of aquatic invertebrates [21]. Recognizing the important role of ecdysteroids in crustacean endocrinology [22,23], we currently selected 20‐hydroxyecdysone (20‐HEC) as a reference compound, which has been shown to affect daphnid and copepod development [24,25] and testosterone metabolism in daphnids [26].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Life‐cycle studies with marine copepods (Tisbe battagliai) exposed to 20‐hydroxyecdysone and diethylstilbestrol

Hutchinson

Pounds

Hampel

et al. 1999

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Abstract-Life-cycle effects of a reference ecdysteroid, 20-hydroxyecdysone (20-HEC) and the model xenoestrogen, diethylstilbestrol (DES) in Tisbe battagliai (Crustacea, Copepoda, Harpacticoida) have been evaluated. T. battagliai is a sexually reproducing species that is representative of an ecologically diverse group of aquatic Crustacea and is highly amenable to laboratory life-cycle studies. Newly released (Ͻ24-h old) Copepod nauplii were exposed to 20-HEC or DES and effects monitored in terms of survival, development, and sex ratio after 10 d at 20 Ϯ 1ЊC. A mortality of ഠ40% occurred after 9-d exposure to 20-HEC at 269 g/L, whereas significant (17.5%) mortality (p Ͻ 0.05) occurred after 4-d exposure to DES at 100 g/L. Adult males and females were paired after day 10, and exposures continued to investigate effects on reproductive output (21-d total exposure). In summary, the 21-d LC50 values (with 95% confidence intervals) for 20-HEC and DES were 53.4 g/L (36.5-78.7) and 31.6 g/L (10-100), respectively. For 20-HEC, the 21-d no observed effect concentration (NOEC) for survival was 26.9 g/L, whereas reproduction was a more sensitive endpoint (NOEC ϭ 8.7 g/L). For DES, survival and reproduction were equally sensitive, and both gave an NOEC value of 10 g/L (all based on nominal concentrations). These results suggest that the 21-d life-cycle protocol using T. battagliai holds much promise as an in vivo test for developmental and reproductive effects in aquatic Crustacea.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Life‐cycle studies with marine copepods (Tisbe battagliai) exposed to 20‐hydroxyecdysone and diethylstilbestrol

Hutchinson

Pounds

Hampel

et al. 1999

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

show abstract

“…Chronic tests (>96 h) with marine and meiobenthic copepods have generally used test endpoints spanning full lifecycle and reproductive conditions in addition to lethality. Hutchinson and Williams [40] investigated the chronic toxicity of industrial effluent discharges to adult females and nauplii of the benthic copepod Tisbe battaglia . They measured effects on female productivity and survival over 9 d and naupliar survival over 7 d, concluding that naupliar survival appeared to be a more sensitive indicator of effluent toxicity than adult reproduction.…”

Section: Discussionmentioning

confidence: 99%

Acute and chronic bioassays with New Zealand freshwater copepods using pentachlorophenol

Willis

1999

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

The suitability for laboratory culture and comparative sensitivity of three species of New Zealand freshwater copepod (Calamoecia lucasi Brady, Boeckella delicata Percival, and Mesocyclops cf. leuckarti Claus) to pentachlorophenol (PCP) was assessed. Acute bioassays used two life stages (nauplii and adults). Acute 48‐h lethality tests were conducted at 22°C with laboratorycultured animals of all species and at varying temperatures with seasonally collected C. lucasi adults. Mean 48‐h median lethal concentration values for nauplii ranged from 52 to 227 μg/L PCP for C. lucasi and B. delicata, respectively, and from 106 to 173 μg/L for adult C. lucasi and M. leuckarti, respectively. The survival rate in controls was ≥95% in acute tests, with the exception of C. lucasi nauplii, in which it was 60%. Mean 48‐h median lethal concentration values for seasonally collected C. lucasi adults were significantly higher in summer than in all other seasons. Chronic sublethal tests starting with nauplii <24 h old measured time to metamorphosis. Pentachlorophenol delayed metamorphosis in all species. Chronic toxicity values were 14, 61, and 104 μg/L PCP for C. lucasi, B. delicata, and M. leuckarti, respectively. The mortality rate in controls was also high in C. lucasi sublethal tests (65%), and of the three species, they were the most difficult to culture.

show abstract

“…The meiobenthic copepod T. battagliai has been used as a test organism since 1987 for testing of sediment elutriates (Williams 1992), industrial effluents (Hutchinson and Williams 1989) and chemicals (Barata et al 2002a). These three papers describe a test design using reproductive parameters in individually held females and survival of early life stages as ecotoxicological endpoints.…”

Section: Methods Using Marine Copepodsmentioning

confidence: 99%

Towards an internationally harmonized test method for reproductive and developmental effects of endocrine disrupters in marine copepods

Kusk

Wollenberger

2007

Ecotoxicology

View full text Add to dashboard Cite

New and updated methods to detect and characterize endocrine disrupting chemicals (EDCs) are urgently needed for the purpose of environmental risk assessment since these substances are often not detected using existing chronic toxicity tests. Numerous reports on the effects of EDCs on crustacean development and reproduction have been published and the development of life-cycle tests with crustaceans has been prioritized within the OECD work program for endocrine disrupter testing and assessment. As a result, Sweden, and Denmark initiated a proposal for development of a full life-cycle test with marine copepods (Acartia tonsa, Nitocra spinipes, Tisbe battagliai, and Amphiascus tenuiremis). The present paper gives an overview on the endocrine system of crustaceans with special emphasis on development and reproduction, which are targets for endocrine disruption, and reviews available methods for detecting effects on development and reproduction in calanoid and harpacticoid copepods. A draft OECD guideline Copepod Development and Reproduction Test has been developed, and a pre-validation of this draft guideline was completed in 2005. An updated draft guideline, taking into account the results of the pre-validation, is now under validation in an international ring-test, which is running till the end of 2006.

show abstract

The use of sheepshead minnow (Cyprinodon variegatus) and a benthic copepod (Tisbe battagliai) in short-term tests for estimating the chronic toxicity of industrial effluents

Cited by 15 publications

References 6 publications

Life‐cycle studies with marine copepods (Tisbe battagliai) exposed to 20‐hydroxyecdysone and diethylstilbestrol

Life‐cycle studies with marine copepods (Tisbe battagliai) exposed to 20‐hydroxyecdysone and diethylstilbestrol

Acute and chronic bioassays with New Zealand freshwater copepods using pentachlorophenol

Towards an internationally harmonized test method for reproductive and developmental effects of endocrine disrupters in marine copepods

Contact Info

Product

Resources

About