The use of seed protein electrophoresis in the study of phylogenetic relationships in Chili pepper (Capsicum L.)

Panda, Rames C.; Kumar, O. Aniel; Rao, K. G. Raja

doi:10.1007/bf00289006

Cited by 25 publications

(12 citation statements)

References 21 publications

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“…Early studies investigating genetic diversity in the genus (wild and domesticated species) used protein-or allozymebased molecular markers (Loaiza-Figueroa et al, 1989;Panda et al, 1986). These were subsequently replaced by DNA-based molecular markers such as restriction fragment length polymorphism [RFLP (Lefebvre et al, 1993;Prince et al, 1995)], RAPD Bosland, 2002, 2004;Buso et al, 2003;Kang et al, 1997;Prince et al, 1995;Rodriguez et al, 1999;Votava and Bosland, 2001;Votava et al, 2005), and amplified fragment length polymorphism [AFLP (Guzmán et al, 2005;Lanteri et al, 2003;Paran et al, 1998;Toquica et al, 2003)].…”

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Genetic Structure and Phylogenetic Relationships of Capsicum chinense

Moses¹,

Umaharan²

2012

J. Amer. Soc. Hort. Sci.

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Capsicum chinense is commercially the most important pepper species grown in the Caribbean. It is popularly used to impart pungency and flavor to Caribbean cuisine. However, unlike Capsicum annuum, which is the most commercially exploited domesticated species internationally, C. chinense has not been methodically collected or characterized for systematic improvement through plant breeding. The objectives of the study were to assess the diversity of C. chinense and its structure within the Caribbean basin and to determine its phylogenetic relationship to groups within South America. DNA isolated from 201 accessions of C. chinense, representing geographical regions where the species is found, were amplified using arbitrary primers to generate 138 polymorphic and reproducible random amplification of polymorphic DNA (RAPD) markers. Nei’s and Shannon’s diversity indices for C. chinense (0.28 and 0.419, respectively) were higher in South America compared with Central America or the Caribbean, corresponding to its putative center of diversity. The study showed the existence of three phylogenetic clusters within C. chinense. The largest cluster consisted of accessions from the Upper Amazon region, the Guianas including Venezuela, and the Lesser Antilles of the Caribbean. The other major cluster was represented by accessions principally from the Lower Amazon region. Another distinct but small cluster consisted of samples solely from the Greater Antilles of the Caribbean. The discovery of the three phylogenetic clusters within C. chinense may have potential for exploiting heterosis in breeding. The implications of the findings to the understanding of the phylogenetic origin and distribution of C. chinense are discussed.

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Genetic Structure and Phylogenetic Relationships of Capsicum chinense

Moses¹,

Umaharan²

2012

J. Amer. Soc. Hort. Sci.

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“…Furthermore, some morphological traits are influenced by environment. Chromosome morphology (Pickersgill, 1971), electrophoresis of soluble proteins (Panda et al, 1986;Shin et al, 1989), and isozymes (Jensen et al, 1979;Loiaza et al, 1989) have been used to study diversity in the genus Capsicum. Recently, use of DNA based markers are being used increasingly for this purpose.…”

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Genetic Diversity of a Capsicum Germplasm Collection from Nepal as Determined by Randomly Amplified Polymorphic DNA Markers

Baral¹,

Bosland²

2002

jashs

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ADDITIONAL INDEX WORDS. chile, pepper, cluster analysis, polymorphism, genetic similarity ABSTRACT. Domesticated chile (Capsicum annuum L. var. annuum) is a widely cultivated spice and vegetable crop. It originated in the Western Hemisphere, but spread rapidly throughout the globe after the voyage of Columbus. However, very little is known about the genetic diversity of chile in Asia and especially in Nepal. Thus, research was conducted to document morphological as well as molecular characterization of C. annuum var. annuum landraces collected from Nepal. Genetic diversity in C. annuum var. annuum landraces from Nepal was investigated using randomly amplified polymorphic DNA (RAPD) markers and compared with that of C. annuum var. annuum landraces from the center of diversity, Mexico. RAPD marker based cluster analysis of C. annuum var. annuum clearly separated each accession. All accessions of C. annuum var. annuum from Nepal grouped into a single cluster at a similarity index value of 0.80, whereas, accessions from Mexico grouped into eight different clusters at the same similarity level indicating greater genetic diversity in Mexican accessions. RAPD analysis indicated that the Nepalese chile population went through an additional evolutionary bottleneck or founder effect probably due to intercontinental migrations. Some Nepalese accessions had unique RAPD markers suggesting that additional sources of genetic variation are available in Nepalese germplasm. A contribution of the New Mexico State University Agricultural Experiment Station. The authors wish to thank Pim Lindhout for reviewing the manuscript and providing valuable suggestions for improvement.

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“…The dendogram constructed under SDS-PAGE buffer extract grouped C. mannii and C. edulis according to their genus which is in agreement with the taxonomic classification of these species. SDS-PAGE profiles of buffer soluble seed proteins is thus the best method for the study of genetic diversity and has been utilized by other investigators to study genetic diversity in a number of crops (Panda et al, 1986;Panella et al,1993;Sammour, 1991;Javid et al, 2004;Iqbal et al, 2005;Sheidai et al, 2000Sadia et al,2009Ghafoor et al,2002;Koenig et al,1990;Karihaloo et al, 2002;Lioli et al, 2005;Aiken et al, 1998;Yüzbaşıoğlu et al, 2008).…”

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confidence: 99%

Evaluation of Genetic Diversity within Some Cucurbitaceae Seeds from Nigeria using Electrophoresis

Lukong¹,

Udedi²,

Ezebuo³

et al. 2018

TJANS

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Taxonomic relationships within seven species of Cucurbitaceae; melon seeds were examined at the molecular level with the help of seed storage proteins using electrophoretic methods. Water soluble seed proteins were resolved into 99 and 218 bands respectively under Native-and SDS-PAGE. Similarly 132 and 123 bands respectively were observed for buffer soluble proteins. For water soluble proteins under Native-PAGE, the Jaccard's similarity indexes indicated that Lagenaria siceraria/Cucurbita moschata showed highest similarity index (~96%) while Cucumis sativus/Colocynthis vulgaris showed the lowest similarity index (~35%). For buffer extracts, highest similarity index (~81%) was exhibited by C. sativus/C. maxima while C. sativus /C. mannii showed lowest similarity index (~51%). For water soluble proteins under SDS-PAGE, highest similarity index (~78%) was found between C. maxima /C. vulgaris and the lowest similarity index (34%) was observed between C. sativus /L. siceraria. For buffer extracts, highest similarity index (~98%) was exhibited by C. mannii /C. moschata while C. maxima/C. vulgaris showed the lowest similarity index (~41%). However, from the dendograms constructed, buffer soluble proteins under SDS-PAGE proved to be the best method as it was the only one that grouped C. mannii and C. edulis according to their taxonomic classification.

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The use of seed protein electrophoresis in the study of phylogenetic relationships in Chili pepper (Capsicum L.)

Cited by 25 publications

References 21 publications

Genetic Structure and Phylogenetic Relationships of Capsicum chinense

Genetic Structure and Phylogenetic Relationships of Capsicum chinense

Genetic Diversity of a Capsicum Germplasm Collection from Nepal as Determined by Randomly Amplified Polymorphic DNA Markers

Evaluation of Genetic Diversity within Some Cucurbitaceae Seeds from Nigeria using Electrophoresis

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