Genetic Diversity of a Capsicum Germplasm Collection from Nepal as Determined by Randomly Amplified Polymorphic DNA Markers

Baral, Jit B.; Bosland, Paul W.

doi:10.21273/jashs.127.3.318

Cited by 23 publications

(15 citation statements)

References 22 publications

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“…Accessions of C. annuum var. annuum from the center of diversity, i.e., Mexico, have a lower average genetic similarity of 0.75 (Baral and Bosland, 2002).…”

Section: Discussionmentioning

confidence: 99%

Unraveling the Species Dilemma in Capsicum frutescens and C. chinense (Solanaceae): A Multiple Evidence Approach Using Morphology, Molecular Analysis, and Sexual Compatibility

Baral¹,

Bosland²

2004

jashs

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The species status of two morphologically closely related species, Capsicum frutescens L. and C. chinense Jacq., was investigated using typological, phylogenetic, and biological species concepts. Diagnostic morphological differences, randomly amplified polymorphic DNA (RAPD) marker-based cluster analysis, and hybrid analyses were used to delimit C. frutescens and C. chinense. In many cases two morphological characters, calyx constriction and flower position, can separate accessions of C. frutescens from C. chinense. The RAPD-based analysis clearly separated accessions of C. frutescens and C. chinense into two distinct groups. The average genetic similarity within C. frutescens and C. chinense accessions was 0.85 and 0.8, respectively, whereas the average genetic similarity between C. frutescens and C. chinense accessions was only 0.38. The progenies obtained from C. frutescens and C. chinense hybridization had reduced fertility. Based on these evidences, C. frutescens and C. chinense represent two morphologically diagnosable, phylogenetically distinct, and reproductively isolated species.

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“…Accessions of C. annuum var. annuum from the center of diversity, i.e., Mexico, have a lower average genetic similarity of 0.75 (Baral and Bosland, 2002).…”

Section: Discussionmentioning

confidence: 99%

Unraveling the Species Dilemma in Capsicum frutescens and C. chinense (Solanaceae): A Multiple Evidence Approach Using Morphology, Molecular Analysis, and Sexual Compatibility

Baral¹,

Bosland²

2004

jashs

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“…When seedlings had eight to 10 true leaves, six plants were randomly selected from each accession for DNA isolation. DNA extraction, quantification, and RAPD procedures, including the polymerase chain reaction thermal cycling profile used in this study, are described by Baral and Bosland (2002). Operon primers used were OPP7, OPF6, OPAI1, OPAJ11, OPAD12, OPAJ14, OPA4, OPAD6, OPAK4, OPM4, OPAN11, OPAE19, and OPC2.…”

Section: Methodsmentioning

confidence: 99%

‘Bhut Jolokia’—The World's Hottest Known Chile Pepper is a Putative Naturally Occurring Interspecific Hybrid

Bosland¹,

Baral²

2007

horts

116

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In replicated trials at Las Cruces, N.M., the Scoville heat units (SHUs) of ‘Bhut Jolokia’, a chile pepper from Assam, India, reached one million SHUs. Morphologic characters revealed that ‘Bhut Jolokia’ is a Capsicum chinense Jacq. cultivar. Molecular analysis with randomly amplified polymorphic DNA markers confirmed the species identification and, interestingly, revealed that there may have been genetic introgression from Capsicum frutescens L. into ‘Bhut Jolokia’.

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“…RAPD marker has proven quite useful in genetic study of many plant species [9]. This marker system has the ability to amplify DNA from dispersed polymorphic loci and has its power to detect small genetic differences [10].…”

Section: Introductionmentioning

confidence: 99%

RAPD and Internal Transcribed Spacer Sequence Analyses Reveal Zea nicaraguensis as a Section Luxuriantes Species Close to Zea luxurians

Wang

Zheng

et al. 2011

PLoS ONE

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Genetic relationship of a newly discovered teosinte from Nicaragua, Zea nicaraguensis with waterlogging tolerance, was determined based on randomly amplified polymorphic DNA (RAPD) markers and the internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA using 14 accessions from Zea species. RAPD analysis showed that a total of 5,303 fragments were produced by 136 random decamer primers, of which 84.86% bands were polymorphic. RAPD-based UPGMA analysis demonstrated that the genus Zea can be divided into section Luxuriantes including Zea diploperennis, Zea luxurians, Zea perennis and Zea nicaraguensis, and section Zea including Zea mays ssp. mexicana, Zea mays ssp. parviglumis, Zea mays ssp. huehuetenangensis and Zea mays ssp. mays. ITS sequence analysis showed the lengths of the entire ITS region of the 14 taxa in Zea varied from 597 to 605 bp. The average GC content was 67.8%. In addition to the insertion/deletions, 78 variable sites were recorded in the total ITS region with 47 in ITS1, 5 in 5.8S, and 26 in ITS2. Sequences of these taxa were analyzed with neighbor-joining (NJ) and maximum parsimony (MP) methods to construct the phylogenetic trees, selecting Tripsacum dactyloides L. as the outgroup. The phylogenetic relationships of Zea species inferred from the ITS sequences are highly concordant with the RAPD evidence that resolved two major subgenus clades. Both RAPD and ITS sequence analyses indicate that Zea nicaraguensis is more closely related to Zea luxurians than the other teosintes and cultivated maize, which should be regarded as a section Luxuriantes species.

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Genetic Diversity of a Capsicum Germplasm Collection from Nepal as Determined by Randomly Amplified Polymorphic DNA Markers

Cited by 23 publications

References 22 publications

Unraveling the Species Dilemma in Capsicum frutescens and C. chinense (Solanaceae): A Multiple Evidence Approach Using Morphology, Molecular Analysis, and Sexual Compatibility

Unraveling the Species Dilemma in Capsicum frutescens and C. chinense (Solanaceae): A Multiple Evidence Approach Using Morphology, Molecular Analysis, and Sexual Compatibility

‘Bhut Jolokia’—The World's Hottest Known Chile Pepper is a Putative Naturally Occurring Interspecific Hybrid

RAPD and Internal Transcribed Spacer Sequence Analyses Reveal Zea nicaraguensis as a Section Luxuriantes Species Close to Zea luxurians

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