The use of real-time PCR for quantitative determination of some propionic bacteria inhabiting the human skin

Globa, A. G.; Alekseev, Ya. I.; Arzumanyan, V. G.; Zaborova, Victoria; Guridov, A. A.

doi:10.1134/s1990750813020066

Cited by 2 publications

(3 citation statements)

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“…Flasks were seeded with culture from Petri dishes by syringe needle and incubated at 32°С without agitation. Aliquots of cultural fluid were periodically selected during growth period and their optical density was measured by spectrophotometer («Genesis 10S UV-Vis», USA) at 530 nm, width of cuvette 1 cm [4].…”

Section: Strains and Cultivationmentioning

confidence: 99%

“…For quantitative analysis of Ptgt 2 plasmid contained the insertion of unique sequence, the specific primers and probe labelled with fluorescent dye Cy5 were used [4]. The required oligonucleotides were synthesized by "Synthol", Russia.…”

Section: Real Time Pcrmentioning

confidence: 99%

“…Thereupon we have used the real time PCR method [3] in modification [4], which allowed to quantitatively identify all the three species of skin propionic bacteria. Use of this method allowed us to determine that propionic bacteria of more than 50 hosts are the mixtures of all three species simultaneously.…”

Section: Introductionmentioning

confidence: 99%

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Communities of Skin Propionic Bacteria: Cultivation and Antifungal Antagonistic Activity

Arzumanyan¹,

Zaborova²

2016

J Bacteriol Parasitol

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Many studies concerned the propionic bacteria of human skin report just about Propionibacterium acnes, whereas it is well known that the species diversity include at least three species-P. acnes, P. granulosum and P. avidum. Recently we have used real time PCR method, which allow detecting simultaneously the three species in skin washings of healthy people. At the first step of the study we tried to isolate pure culture of P. acnes. From 7 swabs from healthy skin propionic bacteria one was selected for cultivation, because it contained according to real time PCR only cells of P. acnes. During the cultivation of this isolate in liquid medium probes were collected and again analyzed by real time PCR. All three species of propionic bacteria were detected in each phase of cultivation. In our opinion this fact may be a reason to check the collection strains named P. acnes towards their species "purity". Antagonism of different propionibacteria against bacteria is well known, however so far nothing have published about their antifungal activity. The strain of Candida albicans was chosen as the test culture. Fungal cells were exposed with cell-free liquid of last stationary phase culture of propionic bacteria. About 59.4 % of alive fungal cells were killed during 2 hours when aliquot of C. albicans was combined with aliquot of bacterial cultural cell-free liquid. Propionic bacteria excreted at least two antifungal components-thermolabile and thermostabile. Small part of activity had molecular mass 3 ÷ 10 kDa, whereas the main part was contained in the fraction less than 3 kDa. Thus a new type of antagonistic activity of propionic bacteria was found out fungicidal activity, which confirm the existence of protective function of skin commensal microbiota.

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Section: Strains and Cultivationmentioning

confidence: 99%

Section: Real Time Pcrmentioning

confidence: 99%