The use of RAPD markers for detecting genetic diversity, relationship and molecular identification of Chinese elite tea genetic resources [Camellia sinensis (L.) O. Kuntze] preserved in a tea germplasm repository

Abstract

The genetic diversity, relationship and molecular identification of 15 well known, widely planted traditional Chinese elite tea genetic resources [Camellia sinensis (L.) O. Kuntze] preserved in the China National Germplasm Hangzhou Tea Repository in the Tea Research Institute of the Chinese Academy of Agricultural Sciences located in Zhejiang province, China, were investigated using RAPD markers. A total of 1050 bands with an average of 52.5 bands per primer, 70 bands per genetic resource were generated by the 20 selected primers from the 15 tea genetic resources. In the total of 137 amplified products, 129 were polymorphic, corresponding to 94.2% genetic diversity. The relative frequency of polymorphic products was from 0.24 to 0.83, with an average of 0.47. In general, this average frequency was relatively high. The genetic distances among the genetic resources were from 0.16 to 0.62, with an average of 0.37. The 15 tea genetic resources were grouped into three groups by UPGMA cluster analysis based on RAPD data. By using the presence of 20 unique RAPD markers and the absence of 11 unique markers, all the 15 investigated tea genetic resources could be easily identified. RAPD markers provided a practical method not only to evaluate the genetic diversity and relationship, but also to identify tea genetic resources.

Heterozygosities and genetic relationship of tea cultivars revealed by simple sequence repeat markers and implications for breeding and genetic mapping programs

“…This result is consistent with other molecular marker studies of C. sinensis (Chen et al, 2005;Fang et al, 2012;Yao et al, 2012), and this result is probably because genetic exchanges occurred frequently among Fujian, Zhejiang, and Hunan because of human activities.…”

Generation and characterization of 24 novel EST derived microsatellites from tea plant (Camellia sinensis) and cross-species amplification in its closely related species and varieties

“…A great number of tea germplasms, including the tea plant and its allied species and varieties in section Thea genus Camellia have been collected and conserved in China, Japan, India, Kenya, etc. (Chen et al 2005a). It is of great importance for the well management and conservation studies of tea germplasms for the successful utilization.…”

Keragaman Genetik Beberapa Genotipe Teh Berdasarkan Penanda RAPD (Random Amplified Polymorphic DNA)

“…Teh memiliki keragaman genetik yang luas (Chen, Gao, Chen, & Xu, 2005;Thomas, Kumar, & Mandal, 2006;Jain & Priyadarshan, 2009), dan hal ini dapat dilihat dari habitus, morfologi, dan kandungan kimianya. Hasil evaluasi beberapa genotipe teh menunjukkan perbedaan daya hasil, kandungan kimia, dan ketahanan terhadap hama penyakit (Martono, Rubiyo, Setiyono, & Udarno, 2013 (Karp, Seberg, & Buiatti, 1996;Kaundun, Zhyvoloup, & Young-Goo, 2000;Chen, Qi, Li, Zou, & Shan, 2012).…”