The use of quaternised chitosan-loaded PMMA to inhibit biofilm formation and downregulate the virulence-associated gene expression of antibiotic-resistant staphylococcus

Tan, Honglue; Peng, Zhaoxiang; Li, Qingtian; Xu, Xiaojuan; Guo, Shengrong; Tang, Tingting

doi:10.1016/j.biomaterials.2011.09.084

Cited by 205 publications

(173 citation statements)

References 54 publications

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“…33 The solutions were serially diluted and re-cultivated in triplicate on sheep blood agar plates (for F. nucleatum) or agar plates (for S. aureus) for 48 hours and 24 hours, respectively. 24 The number of CFUs on the agar plates was counted in accordance with the National Standard of China GB/T 4789.2 protocol.…”

Section: Release Of Silvermentioning

confidence: 99%

Hierarchical micro/nanostructured titanium with balanced actions to bacterial and mammalian cells for dental implants

Zhu

Cao

Qiao

et al. 2015

IJN

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A versatile strategy to endow dental implants with long-term antibacterial ability without compromising the cytocompatibility is highly desirable to combat implant-related infection. Silver nanoparticles (Ag NPs) have been utilized as a highly effective and broad-spectrum antibacterial agent for surface modification of biomedical devices. However, the high mobility and subsequent hazardous effects of the particles on mammalian cells may limit its practical applications. Thus, Ag NPs were immobilized on the surface of sand-blasted, large grit, and acid-etched (SLA) titanium by manipulating the atomic-scale heating effect of silver plasma immersion ion implantation. The silver plasma immersion ion implantation-treated SLA surface gave rise to both good antibacterial activity and excellent compatibility with mammalian cells. The antibacterial activity rendered by the immobilized Ag NPs was assessed using Fusobacterium nucleatum and Staphylococcus aureus , commonly suspected pathogens for peri-implant disease. The immobilized Ag NPs offered a good defense against multiple cycles of bacteria attack in both F. nucleatum and S. aureus , and the mechanism was independent of silver release. F. nucleatum showed a higher susceptibility to Ag NPs than S. aureus , which might be explained by the presence of different wall structures. Moreover, the immobilized Ag NPs had no apparent toxic influence on the viability, proliferation, and differentiation of rat bone marrow mesenchymal stem cells. These results demonstrated that good bactericidal activity could be obtained with very small quantities of immobilized Ag NPs, which were not detrimental to the mammalian cells involved in the osseointegration process, and promising for titanium-based dental implants with commercial SLA surfaces.

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Section: Release Of Silvermentioning

confidence: 99%

Hierarchical micro/nanostructured titanium with balanced actions to bacterial and mammalian cells for dental implants

Zhu

Cao

Qiao

et al. 2015

IJN

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“…Cheng et al [61] designed a switchable polymer that is toxic to bacteria on contact, then switches to a nonfouling zwitterionic coating to release the debris and maintain a non-fouled surface. Tan et al [62] have also developed a chitosan derivative with a series of substituted quaternary ammonium groups that can be loaded into PMMA to decrease fouling by antibiotic-resistant Staphylococcus aureus. D-amino acids have become increasingly popular as a signaling molecule for biofilm dispersal.…”

Section: Antifouling Agentsmentioning

confidence: 99%

Perspectives on the prevention and treatment of infection for orthopedic tissue engineering applications

2013

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“…baumannii, both tested in this work, are resistant to these drugs, 44,45 rendering the use of these two antibiotics ineffective when such strains are involved. We tested the three types of bone cements supplemented with gentamicin 2% w/w (the commercial formulation of this antibiotic in bone cement) against the four pathogens, and the growth of S. epidermidis was not prevented ( Figure S4).…”

mentioning

confidence: 97%

“…Our results demonstrated that organic nanoparticles made of propylparaben are effective against a wide range of bacteria, including antibiotic-resistant strains (Figures 3 and 4) found in orthopedic infections; hence, the use of these organic nanoparticles could offer not only a possible alternative to antibiotics, but also solve some of the problems already associated with antibiotic resistance. Moreover, the amount of paraben nanoparticles required to provide effective antimicrobial activity is similar to the amount of gentamicin and tobramycin used (2%-4% w/w) and is significantly lower than other antimicrobial agents, such as chitosan 11 and quaternized chitosan derivative, 44 that require about 20%-30% w/w. Despite providing antimicrobial activity, in order to be a viable option, the organic nanoparticles must not induce negative effects on the other bone cement properties.…”

mentioning

confidence: 99%

Antimicrobial activity of bone cements embedded with organic nanoparticles

Perni¹,

Thenault²,

Abdo³

et al. 2015

IJN

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Infections after orthopedic surgery are a very unwelcome outcome; despite the widespread use of antibiotics, their incidence can be as high as 10%. This risk is likely to increase as antibiotics are gradually losing efficacy as a result of bacterial resistance; therefore, novel antimicrobial approaches are required. Parabens are a class of compounds whose antimicrobial activity is employed in many cosmetic and pharmaceutical products. We developed propylparaben nanoparticles that are hydrophilic, thus expanding the applicability of parabens to aqueous systems. In this paper we assess the possibility of employing paraben nanoparticles as antimicrobial compound in bone cements. The nanoparticles were embedded in various types of bone cement (poly(methyl methacrylate) [PMMA], hydroxyapatite, and brushite) and the antimicrobial activity was determined against common causes of postorthopedic surgery infections such as: Staphylococcus aureus , methicillin-resistant S. aureus , Staphylococcus epidermidis , and Acinetobacter baumannii . Nanoparticles at concentrations as low as 1% w/w in brushite bone cement were capable of preventing pathogens growth, 5% w/w was needed for hydroxyapatite bone cement, while 7% w/w was required for PMMA bone cement. No detrimental effect was determined by the addition of paraben nanoparticles on bone cement compression strength and cytocompatibility. Our results demonstrate that paraben nanoparticles can be encapsulated in bone cement, providing concentration-dependent antimicrobial activity; furthermore, lower concentrations are needed in calcium phosphate (brushite and hydroxyapatite) than in acrylic (PMMA) bone cements. These nanoparticles are effective against a wide spectrum of bacteria, including those already resistant to the antibiotics routinely employed in orthopedic applications, such as gentamicin.

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The use of quaternised chitosan-loaded PMMA to inhibit biofilm formation and downregulate the virulence-associated gene expression of antibiotic-resistant staphylococcus

Cited by 205 publications

References 54 publications

Hierarchical micro/nanostructured titanium with balanced actions to bacterial and mammalian cells for dental implants

Hierarchical micro/nanostructured titanium with balanced actions to bacterial and mammalian cells for dental implants

Perspectives on the prevention and treatment of infection for orthopedic tissue engineering applications

Antimicrobial activity of bone cements embedded with organic nanoparticles

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