The use of proteases from extreme thermophiles for meat tenderisation

Wilson, Shelley-Ann; Young, O.A.; Coolbear, Tim; Daniel, Roy M.

doi:10.1016/0309-1740(92)90019-z

Cited by 25 publications

(10 citation statements)

References 17 publications

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“…It represents one of the three largest groups of industrial enzymes (Mohsen et al 2005) and constitutes 60-65% of the global industrial enzyme market (Nunes and Martins 2001;Singh et al 2001;Leikus et al 1998). Proteases are mostly used in enzyme-containing detergent products (Kalisz 1988), food-processing (Pastor et al 2001) and pharmaceutical industries (Anwar and Saleemuddin 1998;Gupta et al 2002), meat tenderization process (Takagi et al 1992;Wilson et al 1992), peptide synthesis (Kumar and Hiroshi 1999), infant formula preparation (American Academy of Pediatrics Committee on Nutrition 1989), leather processing (George et al 1995) and recovery of silver from X-ray films (Ishikawa et al 1993), etc.…”

Section: Introductionmentioning

confidence: 98%

Production and characterization of extracellular protease of mutant Aspergillus niger AB100 grown on fish scale

Basu

Banik

Das

2007

World J Microbiol Biotechnol

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Fish scale, the chief waste material of fish processing industries was processed and tested for production of extracellular protease by mutant Aspergillus niger AB 100 . Protease production by A. niger AB 100 was greatly enhanced in presence of processed fish scale powder. Where as among the three complex nutrients tested, soya bean meal shows maximum stimulatory effect over protease production (2,776 lmol/ml/min) when used in combination with glucose (5% w/v) and urea (2.5% w/v). The protease was optimally active at pH 7.0, retaining more than 60% of its activity in the pH range of 5-9. The enzyme was found to be most active at 50°C and stable at 30°C for 1 h. Purification of enzyme by CM-Cellulose and SDS-PAGE resulted in about 26-fold increase in the specific activity of the enzyme with a molecular weight of 30.9 kDa. HPLC study shows the purity of the enzyme as 75.92%. By the activating effect of divalent cations (Fe 2+ , Zn 2+ , Mn 2+ , Ca 2+ and Mg 2+ ) and inhibiting effect of chelating agent (EDTA) and Hg 2+ , the enzyme was found to be a metalloprotease.

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Section: Introductionmentioning

confidence: 98%

Production and characterization of extracellular protease of mutant Aspergillus niger AB100 grown on fish scale

Basu

Banik

Das

2007

World J Microbiol Biotechnol

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“…Among these, alkaline protease is of particular interests due to its broad applications in detergent, tanning, and dairy industries [2][3][4]. Since 50-90% of production cost resides in the purification strategy, many procedures have been developed for alkaline protease downstream processing [5].…”

Section: Introductionmentioning

confidence: 99%

Extraction of alkaline protease using an aqueous two-phase system from cell free Bacillus subtilis TISTR 25 fermentation broth

Chouyyok

Wongmongkol

Siwarungson

et al. 2005

Process Biochemistry

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“…Also temperature stability of the immobilized protease compared to the soluble enzyme has showed 15-20% increase as indicated by its inactivation pattern. Upon repeated use, the entrapped enzyme retained 83% of its initial activity after 6 cycles (Wilson et al, 1992). A novel use of alkaline proteases is its proposed usefulness in detection of Pseudomonas aeroginosa in ELISA tests.…”

Section: Introductionmentioning

confidence: 99%

Optimization of cultural conditions for the production of an extracellular protease by Pseudomonas species

Vasantha

Subramanian

2012

Int Curr Pharm J

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The aim of the study was to identify new sources of protease and a protease producing bacteria was isolated from slaughter house soil and identified as Pseudomonas species. The protease production efficiency of the organism was measured with different environmental and nutritional parameters. Optimization of the fermentation medium for maximum protease production was carried out. The culture condition like temperature, pH, incubation time, carbon and nitrogen sources were optimized. The optimum condition found for protease production was 30°C at pH 7 in the medium for 48 hour. Carbon source casein and nitrogen source beef extract stimulated the production of protease. Protease production was higher with immobilized cells. Pseudomonas species can be used profitably for the large scale production of protease to meet the present day demand of the industrial sector.DOI: http://dx.doi.org/10.3329/icpj.v2i1.12870 International Current Pharmaceutical Journal 2012, 2(1): 1-6

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The use of proteases from extreme thermophiles for meat tenderisation

Cited by 25 publications

References 17 publications

Production and characterization of extracellular protease of mutant Aspergillus niger AB100 grown on fish scale

Production and characterization of extracellular protease of mutant Aspergillus niger AB100 grown on fish scale

Extraction of alkaline protease using an aqueous two-phase system from cell free Bacillus subtilis TISTR 25 fermentation broth

Optimization of cultural conditions for the production of an extracellular protease by Pseudomonas species

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