Optimization of cultural conditions for the production of an extracellular protease by Pseudomonas species

Vasantha, Soumya Thrivikraman; Subramanian, A.

doi:10.3329/icpj.v2i1.12870

Cited by 6 publications

(5 citation statements)

References 11 publications

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“…Al-Saleh and Zahran (1999) studied the effect of temperature in the range of 7 to 37 °C on growth and lipase production of psychrotrophic strains of Pseudomonas fluorescens (RM4) and observed that optimum temperature for lipase production was 37 °C. Similar results were obtained by Vasantha et al (2012) who reported protease production at temperature range of 30 -50°C. However, increase in temperature beyond 50° C led to decline in production of enzyme proving that temperature plays a major role in protease production.…”

Section: Resultssupporting

confidence: 90%

Production of cell wall degrading enzymes and antibiotic by Pseudomonads for assessing their biocontrol potential

et al. 2019

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A comprehensive study on bio-control efficacy of different Pseudomonas species was made in terms of cell wall degrading enzymes, viz. chitinase, lipase, protease and antibiotic 2, 4- diacetylphloroglucinol (2,4- DAPG) production abilities in respect to changing temperatures and pH. Observations revealed that all the isolates of Pseudomonas spp. under study produced all the three cell wall degrading enzymes and 2,4- DAPG significantly. However, the isolates PS-11 and PS-30 exhibited maximum enzyme producing abilities. Moreover, PS-11 could be considered to be the maximum heat tolerant among the isolates as it maintain remarkable sustainability even at 55Â° C in the ability to produce enzymes with minimum losses in activities 0.1026 to 0.718 in chitinase, 3.1167 to 2.1794 in lipase and 0.5827 to 0.4075 in protease activities due to rise of temperature from 45Â° C to 55Â° C. In pH variation studied at 35Â°C, it was observed that chitinase, lipase and protease production abilities of most of the isolates of Pseudomonas spp. were maximum at pH value of 6.5 and decreased on further lowering to 5.5 or rising to pH 8.5. PS-30 recorded the highest production of 2,4-DAPG (767.37 Î¼g/ml) at 30Â° C and pH 6.5.

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Section: Resultssupporting

confidence: 90%

Production of cell wall degrading enzymes and antibiotic by Pseudomonads for assessing their biocontrol potential

et al. 2019

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“…The pH of the culture medium strongly affects many enzymatic processes and transport of compounds across the cell membrane [18] during fermentation. Effect of initial pH on production of alkaline protease production and solubility of oxygen in the medium were investigated.…”

Section: Iiiresults and Discussion Production Of Alkaline Protease Imentioning

confidence: 99%

Production of Protease by Alkalophilic Bacillussubtilis in Bioreactor and Its Characterization

Rahman¹,

Anwar²,

Ajmal³

et al. 2017

International Journal of Innovative Research in Advanced Engineering

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ABSTRACT:The current studies were aimed at to investigate role of pH, dissolved oxygen for production protease in bioreactor by alkalophilic bacterium and application of saw dust for its purification. The production of proteolytic enzyme by Bacillus subtilis IC-5 started as pH of medium falls to 9 and reached to maximum at pH 7 i.e., 4400 Uml -1 . Likewise dissolved oxygen decreased in the medium as the protease production progresses. Saw dust was successively utilized for partial purification of protease. The partial purification of protease increased the specific activity to5.3 fold. The optimum pH and temperature for purified activity was 11 and 70 0 C, respectively. The purified enzyme was stable up to pH 12 and 80 o C.

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“…Remains of vegetables, cassava, yam and other plant crops make up plant related market waste. Research has been carried out on many other related sources for enzyme production such as slaughter house waste, dairy effluent and sewage waste [7]. These wastes produced in market places are inexpensive sources for bioconversion processes into products such as enzymes.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Pectinase and Protease Produced from Bacillus subtilis Isolated from Market Waste

Anab-Atulomah

Nwachukwu

2021

SAJRM

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Aims: The objective of the study was to produce and optimize protease and pectinase from Bacillus subtilis isolated from market waste. Place and Duration of Study: Department of Microbiology (laboratory unit), Michael Okpara University of Agriculture Umudike, Abia State Nigeria. Methodology: The production and optimization of protease and pectinase from bacteria isolated from solid market waste was investigated. Isolated bacteria from the waste were screened for protease and pectinase production using skim milk agar and pectin agar respectively. Using morphological, biochemical and molecular technique the enzymes producing isolate was confirmed as Bacillus subtilis. Protease and Pectinase were produced by Bacillus subtilis using submerged fermentation in gelatin broth and pectin broth respectively. The enzymes were purified using ammonium sulphate precipitation, dialysis and ion-exchange chromatography. Optimization using different temperatures, pH and nutrient sources was done. Enzyme activity was measured. Results: Purified protease exhibited maximum activity of 8.72U/ml at 40oC while pectinase exhibited maximum activity of 8.98U/ml at 50oC. Glucose as a carbon source and peptone as a nitrogen source gave optimum activity for both enzymes. Both pectinase and protease exhibited optimum activity at pH 9. There was significant difference (P=.05) in enzyme activity at different temperatures, pH and nitrogen sources for both protease and pectinase. There was no significant difference in pectinase activity at P=.05 for the different carbon sources while there was significant difference for protease activity for the different carbon sources at P=.05. Conclusion: Production of microbial enzymes such as protease and pectinase from waste material is an eco-friendly process and cheaper option for large scale use of enzymes in industry.

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Optimization of cultural conditions for the production of an extracellular protease by Pseudomonas species

Cited by 6 publications

References 11 publications

Production of cell wall degrading enzymes and antibiotic by Pseudomonads for assessing their biocontrol potential

Production of cell wall degrading enzymes and antibiotic by Pseudomonads for assessing their biocontrol potential

Production of Protease by Alkalophilic Bacillussubtilis in Bioreactor and Its Characterization

Optimization of Pectinase and Protease Produced from Bacillus subtilis Isolated from Market Waste

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