Production of Protease by Alkalophilic Bacillussubtilis in Bioreactor and Its Characterization

Abstract

ABSTRACT:The current studies were aimed at to investigate role of pH, dissolved oxygen for production protease in bioreactor by alkalophilic bacterium and application of saw dust for its purification. The production of proteolytic enzyme by Bacillus subtilis IC-5 started as pH of medium falls to 9 and reached to maximum at pH 7 i.e., 4400 Uml -1 . Likewise dissolved oxygen decreased in the medium as the protease production progresses. Saw dust was successively utilized for partial purification of protease. The partial purification of protease increased the specific activity to5.3 fold. The optimum pH and temperature for purified activity was 11 and 70 0 C, respectively. The purified enzyme was stable up to pH 12 and 80 o C.

Strain-magneto-optics of a magnetostrictive ferrimagnetic CoFe2O4

“…In the optical conductivity spectrum (Fig. 2b), the peculiarity at λ = 2.96 μm (0.42 eV) is also observed as well as in the absorption spectra of polycrystalline CoFe 2 O 4 [14]. Similar peaks are found in spectrum of many other complex oxides.…”

Utilization of Extract from Bushcane (Costus Afer) as Green Corrosion Inhibitor for Mild Steel

“…Green corrosion inhibitors, which are biodegradable and free of heavy metals and other harmful substances, are very interesting since organic corrosion inhibitors are poisonous. Utilizing plant extracts, often known as "green corrosion inhibitors," one can successfully slow down the pace of corrosion [13,14]. Generally, green Inhibitors have hetero atoms.…”

“…Because of their greater basicity and electron density, O, N, and S are shown to hinder corrosion. The active centers for the adsorption process on the metal surface are O, N, and S. The order of the inhibition efficiency should be O < N < S < P. The chemical makeup and physical characteristics of the compound, such as the electron density at the donor atom, the porbital nature of the molecule, and its electronic structure, all affect how well a green inhibitor works [12,14]. The inhibition may result from molecules or ions adhering to the metal surface, from an increase or decrease in anodic or cathodic reactions, from a decrease in the rate at which reactants diffuse onto the metal surface, or from the creation of a barrier film that protects the metal [3,27].…”