The use of promoter fusions in Drosophila genetics: Isolation of mutations affecting the heat shock response

Bonner, J. Jose; Parks, Carol I.; Parker-Thornburg, Janice; Mortin, Mark A.; Pelham, Hugh R.B.

doi:10.1016/0092-8674(84)90432-x

Cited by 177 publications

(74 citation statements)

References 33 publications

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“…The reporter construct, herein called pOCA108, contains two full-length CAB3 promoters (see details in Susek et al, 1993) fused to either the alcohol dehydrogenase (ADH) gene from Arabidopsis (Chang and Meyerowitz, 1986) or the Escherichia coli 0-glucuronidase (&A, or GUS) gene (Jefferson, 1987). We chose ADH because both positive and negative genetic selections exist for this enzyme (Bonner et al, 1984). This was important to our screen because we could select against ADH activity using allyl alcohol, which is converted into the toxic aldehyde acrolein.…”

Section: Mutant Lsolation and Genetic Characterizationmentioning

confidence: 99%

CUE1: A Mesophyll Cell-Specific Positive Regulator of Light-Controlled Gene Expression in Arabidopsis.

et al. 1995

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Light plays a key role in the development and physiology of plants. One of the most profound effects of light on plant development is the derepression of expression of an array of light-responsive genes, including the genes encoding the chlorophyll alb binding proteins (CAB) of photosystem II. To understand the mechanism by which light signals nuclear gene expression, we developed a genetic selection to identify mutants with reduced CAB transcription. Here, we describe a new Arabidopsis locus, COE7 (for CAS ynderexpressed). Mutations at this locus result in defects in expression of several light-regulated genes, specifically in mesophyll but not in bundle-associated or epidermis cells. Reduced accumulation of CAB and other photosynthesis-related mRNAs in the mesophyll was correlated with defects in chloroplast development in these cells, resulting in a reticulate pattern with veins greener than the interveinal regions of leaves.Moreover, chalcone synthase mRNA, although known to be regulated by both phytochrome and a blue light receptor, accumulated normally in the leaf epidermis. Dark basal levels of CAB expression were unaffected in etiolated cueí seedlings; however, induction of CAB transcription by pulses of red and blue light was reduced, suggesting that CUEl acts downstream from both phytochrome and blue light photoreceptors. CUEl appears to play a role in the primary derepression of mesophyll-specific gene expression in response to light, because cueí mutants are severely deficient at establishing photoautotrophic growth. Based on this characterization, we propose that CUEí is a cell-specific positive regulator linking light and intrinsic developmental programs in Arabidopsis leaf mesophyll cells.

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Section: Mutant Lsolation and Genetic Characterizationmentioning

confidence: 99%

CUE1: A Mesophyll Cell-Specific Positive Regulator of Light-Controlled Gene Expression in Arabidopsis.

et al. 1995

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“…pCR2 has a XbaI-HmdIII fragment containing the alcohol dehydrogenase structural gene from D. melanogaster (Bonner et al 1984) fused at its HindIII site in the nontranslated leader region to the AvaII site in ypl (+37 of ypl) through a linker (5'-AAGAGGGCTGCAAAGCTCTCTAGAGGTCCTGCGG-3'; the fusion site was verified by sequencing; underlining indicates the Adh sequence beginning at + 21 relative to the larval cap site and the modified HindIII and AvaII sites in 5' to 3' order). The yolk protein component of pCR2 extends to the NcoI site in yp2 ( + 105 of yp2), which is fused to the Sinai site of a modified E. coli lacZ gene [pMLB1034(&AvaI) ~ Shapira et al 1983] by a linker (5'-GCCATGCGGTCGACCGGGGATCCC-3'; underlining indicates the NcoI and SmaI sites remaining in pCR2).…”

Section: Construction Of P-element Plasmidsmentioning

confidence: 99%

Ovarian follicle cell enhancers from the Drosophila yolk protein genes: different segments of one enhancer have different cell-type specificities that interact to give normal expression.

Logan

Wensink²

1990

Genes Dev.

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This paper examines ovarian transcription of the divergently oriented yolk protein genes 1 and 2 (ypl and yp2) of Drosophila melanogaster. We report germ line transformation results demonstrating that ypl and yp2 are transcribed in the same subpopulations of ovarian follicle cells. Our results show that this expression pattern is directed by two enhancers: ovarian enhancer 1, located between the genes, and ovarian enhancer 2, located within the first exon of yp2. Analysis of the expression pattern resulting from alterations in ovarian enhancer 1 demonstrates that different segments of this enhancer have different positive or negative effects on the cell-type specificity of transcription.

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“…First, the 5Ј-untranslated region (5Ј-UTR) of Hsp mRNAs is sufficient to confer efficient translation to a heterologous appended coding region (10,11); and conversely, the Hsp coding sequence and 3Ј-UTR are unable to be translated during heat shock when a non-heat shock 5Ј-UTR, or a mutationally disabled Hsp 5Ј-UTR, precedes it (12,13). Second, the common features found in virtually all Drosophila Hsp mRNA 5Ј-UTRs include: (i) long length (200 -250 nucleotides); (ii) two conserved sequence segments, and positionally conserved nucleotides within the initial element; (iii) a high frequency of adenosine nucleotides (ϳ50%); and (iv) a minimal extent of secondary structure (7).…”

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confidence: 99%

Translational Regulation of Hsp90 mRNA

Ahmed

Duncan

2004

Journal of Biological Chemistry

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Heat shock in Drosophila results in repression of most normal (non-heat shock) mRNA translation and the preferential translation of the heat shock mRNAs. The sequence elements that confer preferential translation have been localized to the 5 -untranslated region (5 -UTR) for Hsp22 and Hsp70 mRNAs (in Drosophila). Hsp90 mRNA is unique among the heat shock mRNAs in having extensive secondary structure in its 5 -UTR and being abundantly represented in the non-heat shocked cell. In this study, we show that Hsp90 mRNA translation is inefficient at normal growth temperature, and substantially activated by heat shock. Its preferential translation is not based on an IRES-mediated translation pathway, because overexpression of eIF4E-BP inhibits its translation (and the translation of Hsp70 mRNA). The ability of Hsp90 mRNA to be preferentially translated is conferred by its 5 -UTR, but, in contrast to Hsp22 and -70, is primarily influenced by nucleotides close to the AUG initiation codon. We present a model to account for Hsp90 mRNA translation, incorporating results indicating that heat shock inhibits eIF4F activity, and that Hsp90 mRNA translation is sensitive to eIF4F inactivation.

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The use of promoter fusions in Drosophila genetics: Isolation of mutations affecting the heat shock response

Cited by 177 publications

References 33 publications

CUE1: A Mesophyll Cell-Specific Positive Regulator of Light-Controlled Gene Expression in Arabidopsis.

CUE1: A Mesophyll Cell-Specific Positive Regulator of Light-Controlled Gene Expression in Arabidopsis.

Ovarian follicle cell enhancers from the Drosophila yolk protein genes: different segments of one enhancer have different cell-type specificities that interact to give normal expression.

Translational Regulation of Hsp90 mRNA

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