The use of pre-enrichment for the isolation of small numbers of gentamicin-resistant Providencia stuartii from faeces

Hawkey, Peter M.; Potten, M.R.; Stephens, Matthew

doi:10.1016/0195-6701(82)90070-6

Cited by 7 publications

(1 citation statement)

References 8 publications

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“…thesis, University of Bristol, Bristol, England). When PIM is combined with suitable liquid enrichment media, which have been reported to greatly enhance the recovery of strains of the Proteeae (9,14), the recovery of small numbers of various species of the Proteeae (including the recently defined species) from specimens such as feces should be greatly improved.…”

Section: Confirmation Of the Occurrence Of Colistin-susceptiblementioning

confidence: 99%

Selective and differential medium for the primary isolation of members of the Proteeae

Hawkey¹,

McCormick²,

Simpson³

1986

J Clin Microbiol

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A new differential and selective medium for the isolation of members of the Proteeae, PIM (Proteeae isolation medium) agar, was developed and evaluated. The medium relies on the ability of all members of the Proteeae (with the exception of a very few Morganella morganii strains) to produce a dark brown pigment in medium containing DL-tryptophan. An additional differential property, tyrosine degradation, was also demonstrated by the medium. Members of the Proteeae appeared as dark brown colonies with a halo of clearing of fine tyrosine crystals when cultured on PIM agar. Occasional strains of Citrobacter sp. and Pseudomonas aeruginosa may degrade tyrosine, but none has the ability to produce dark brown pigmentation on PIM agar. Quantitative recovery studies showed that the addition of 5 mg of clindamycin per liter suppressed gram-positive bacteria without inhibiting any strains of the Proteeae. The addition of 100 mg of colistin per liter made the medium highly selective for strains of the Proteeae, but approximately 10% of the strains were not isolated, thus making this formulation unsuitable for general surveys of the occurrence of members of the Proteeae. PIM agar should aid the investigation of episodes of cross infection caused by members of the Proteeae and the isolation of the new species of the Proteeae recently described.

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Section: Confirmation Of the Occurrence Of Colistin-susceptiblementioning

confidence: 99%

Selective and differential medium for the primary isolation of members of the Proteeae

Hawkey¹,

McCormick²,

Simpson³

1986

J Clin Microbiol

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Providencia

Penner¹

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Pro.vi.den' ci.a . M.L. fem. n. Providencia Providencia, named after the city of Providence, Rhode Island, U.S.A. Proteobacteria / Gammaproteobacteria / Enterobacteriales / Enterobacteriaceae / Providencia Straight rods , 0.6–0.8 × 1.5–2.5 µm, conforming to the general definition of the family Enterobacteriaceae . Gram negative. Motile by peritrichous flagella. They are facultatively anaerobic , chemoorganotrophic, having both a respiratory and a fermentative type metabolism . Optimal growth temperature is 37°C. Oxidatively deaminate phenylalanine and tryptophan. Acid is produced from D ‐mannose. All except Providencia rustigianii produce acid from one or more of the following polyhydric alcohols : inositol, D ‐mannitol, adonitol, D ‐arabitol, and erythritol. Except for Providencia heimbachae all are indole positive. Tartrate (Jordan) is utilized by all but a few strains of P. heimbachae . Simmons citrate is utilized by all species except P. heimbachae and some strains of P. rustigianii . Isolated from diarrhetic stools, urinary tract infections, wounds, burns, bacteremias, and contaminated environmental sources. DNA relatedness tests have shown that Providencia are more closely related to Proteus vulgaris and Proteus mirabilis than to other members of the family Enterobacteriaceae . Although 16S RNA sequence data are not available for the Providencia species, the close relationship of Providencia to Proteus indicates that Providencia , like Proteus , belongs to the Gammaproteobacteria within the family Enterobacteriaceae . The mol % G + C of the DNA is : 39–42 (Falkow et al., 1962). Type species : Providencia alcalifaciens (de Salles Gomes 1944) Ewing 1962, 96 ( Eberthella alcalifaciens de Salles Gomes 1944, 183; Proteus inconstans (Ornstein 1921) Shaw and Clarke 1955, 155.)

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The Genera Proteus, Providencia, and Morganella

Manos¹,

Belas²

2006

The Prokaryotes

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The use of pre-enrichment for the isolation of small numbers of gentamicin-resistant Providencia stuartii from faeces

Cited by 7 publications

References 8 publications

Selective and differential medium for the primary isolation of members of the Proteeae

Selective and differential medium for the primary isolation of members of the Proteeae

Providencia

The Genera Proteus, Providencia, and Morganella

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