The use of poly(l-lactide) and RGD modified microspheres as cell carriers in a flow intermittency bioreactor for tissue engineering cartilage

Chen, Rui; Curran, Stephen J.; Curran, Judith M.; Hunt, John A.

doi:10.1016/j.biomaterials.2006.04.011

Cited by 118 publications

(89 citation statements)

References 30 publications

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“…The spherical biocarriers with relative large specific surface area may provide more growth area for cell culture in vitro. Hepatocytes are difficult to proliferate in monolayer cultures and can be easily damaged by trypsin digestion and may be resolved using spherical biocarriers [29]. In this work, Fourier transform infrared spectroscopy (FT-IR), optical microscopy, high precision surface area, and pore size analyzer were used to characterize the structure and morphology of spherical biocarriers.…”

Section: Introductionmentioning

confidence: 99%

Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility

Zhao

Wang

et al. 2017

International Journal of Polymer Science

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Spherical biocarriers were prepared with lignin-carbohydrate complexes isolated from ginkgo (Ginkgo biloba L.) xylem. The specific surface and average pore size of the biocarriers were 17.15 m 2 g −1 and 21.59 nm, respectively. The carriers were stable in solution at pH 4.0∼9.5. Fourier transform infrared (FT-IR) spectrum indicated that the spherical carrier was composed of lignin and polysaccharides and had a typical lignin-carbohydrate complex (LCC) structure. The contents of galactose, lignin, and total sugar were 3.30%, 23.9%, and 64.62%, respectively, making the spherical biocarriers have good physical strength and compatible with hepatocytes. It was observed using a scanning electron microscopy (SEM) that liver cells adhered to the spherical biocarriers during culture. Cell counting indicated that the proliferation of liver cells in the experimental group was significantly higher than that of the control group. The albumin secretion (ALB) value and glucose consumption of the human hepatocytes were increased by 51.7% and 38.6%, respectively, by the fourth day when cultivated on the biocarriers. The results indicate that ginkgo LCC is very biocompatible and shows promise for the use as a biomaterial in the culture of human hepatocytes.

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Section: Introductionmentioning

confidence: 99%

Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility

Zhao

Wang

et al. 2017

International Journal of Polymer Science

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“…The use of a bioreactor resulted in a significant increase in the number of cells cultured on the biomaterials and the devices studied showed a good capacity to stimulate cell adhesion and proliferation. The authors also observed the formation of microaggregates, a finding that might indicate the production of extracellular matrix [67]. In another study, human mesenchymal stem cells were cultured on a PLDLA scaffold.…”

Section: Tissue Engineering 234mentioning

confidence: 94%

Bioresorbable Polymers for Tissue Engineering

Santos¹

2010

Tissue Engineering

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Polymeric biomaterials are used as substitutes for damaged tissue and for the stimulation of tissue regeneration. One class of polymeric biomaterials are bioresorbable polymers that degrade both in vitro and in vivo and are used as a temporary support for tissue regeneration. Among the various types of bioresorbable polymers, -hydroxy acids including the different forms of poly(lactic acid) (PLA), such as poly(L-lactic acid), poly(Dlactic acid) and poly(DL-lactic acid), as well as poly(glycolic acid) and polycaprolactone, have been extensively studied. These polymers are well known for their good biocompatibility, with their degradation products being eliminated from the body by metabolic pathways. Many reports have shown that the different PLA-based substrates do not present toxicity since the cells were found to differentiate over the different polymers, as demonstrated by the production of extracellular matrix components by various cell types. In this chapter, we describe the use of -hydroxy acids, highlighting the different forms of PLA scaffolds used as cell culture substrates and their applications in clinical practice. The chapter is divided into (1) Introduction; (2) Bioresorbable devices as cell culture substrates; (3) Cell adhesion to polymer substrates; (4) Tissue engineering and bioresorbable polymers; (5) Cell growth and proliferation on bioresorbable polymers; (6) Bioresorbable polymers for cartilage engineering; (7) Bioresorbable polymers for bone tissue engineering; (8) Bioresorbable polymers for skin tissue engineering, and (9) Conclusion.

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“…In some cases the beads are biodegradable, for example, resorbable synthetic polymeric (PLGA) beads (Fig. 3B) have been described, including those with surface modifications to enhance cell attachment (Chen et al, 2006;Hong et al, 2008;Thissen et al, 2005 Natural, extracellular matrix tissues can also be used to make beads for spinner culture, and some, for example highly crosslinked gelatin, Cultispher™, are commercially available. Natural tissue-based beads have a range of advantages compared with synthetic beads, including the presence of natural cell binding and growth factor binding sites, a three dimensional environment including variation in surface topography, in vivo resorption using normal biochemical pathways, and when crosslinking is introduced, control of the resorption rate can be achieved (Glattauer et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Direct Use of Resorbable Collagen-Based Beads for Cell Delivery in Tissue Engineering and Cell Therapy Applications

Glattauer¹,

Tsai²,

White³

et al. 2011

Regenerative Medicine and Tissue Engineering - Cells and Biomaterials

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The use of poly(l-lactide) and RGD modified microspheres as cell carriers in a flow intermittency bioreactor for tissue engineering cartilage

Cited by 118 publications

References 30 publications

Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility

Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility

Bioresorbable Polymers for Tissue Engineering

Direct Use of Resorbable Collagen-Based Beads for Cell Delivery in Tissue Engineering and Cell Therapy Applications

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