The use of oxygen carriers for increasing the production of monoclonal antibodies from hollow fibre bioreactors

Shi, Yijun; Sardonini, Charles A.; Goffe, Randal A.

doi:10.1016/s0923-2494(98)80009-6

Cited by 12 publications

(7 citation statements)

References 15 publications

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“…7,8 The use of O 2 carriers in the cell culture system, on the other hand, provides a biomimetic approach to recapitulate the in vivo oxygenation environment for many tissue engineering applications. [9][10][11] In particular, hepatic hollow fiber (HF) bioreactors that constitute one type of bioartificial liver assist device (BLAD) suffer from O 2 limited transport mainly due to the low solubility of O 2 in the cell culture medium, long diffusion pathlengths, and high demand for O 2 by the hepatocytes cultured in the extracapillary space (ECS). [12][13][14] These devices are expected to bridge patients suffering from acute liver failure toward native liver regeneration or orthotopic liver transplantation by providing sufficient global liver functions.…”

Section: Introductionmentioning

confidence: 99%

Hemoglobin Regulates the Metabolic, Synthetic, Detoxification, and Biotransformation Functions of Hepatoma Cells Cultured in a Hollow Fiber Bioreactor

Chen

Palmer

2010

Tissue Engineering Part A

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Hepatic hollow fiber (HF) bioreactors constitute one type of extracorporeal bioartificial liver assist device (BLAD). Ideally, cultured hepatocytes in a BLAD should closely mimic the in vivo oxygenation environment of the liver sinusoid to yield a device with optimal performance. However, most BLADs, including hepatic HF bioreactors, suffer from O 2 limited transport toward cultured hepatocytes, which reduces their performance. We hypothesize that supplementation of hemoglobin-based O 2 carriers into the circulating cell culture medium of hepatic HF bioreactors is a feasible and effective strategy to improve bioreactor oxygenation and performance. We examined the effect of bovine hemoglobin (BvHb) supplementation (15 g/L) in the circulating cell culture medium of hepatic HF bioreactors on hepatocyte proliferation, metabolism, and varied liver functions, including biosynthesis, detoxification, and biotransformation. It was observed that BvHb supplementation supported the maintenance of a higher cell mass in the extracapillary space, improved hepatocyte metabolic efficiency (i.e., hepatocytes consumed much less glucose), improved hepatocyte capacity for drug metabolism, and conserved both albumin synthesis and ammonia detoxification functions compared to controls (no BvHb supplementation) under the same experimental conditions.

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Section: Introductionmentioning

confidence: 99%

Hemoglobin Regulates the Metabolic, Synthetic, Detoxification, and Biotransformation Functions of Hepatoma Cells Cultured in a Hollow Fiber Bioreactor

Chen

Palmer

2010

Tissue Engineering Part A

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“…The application of the PFD-mediated oxygenation system in Nicotiana tabacum BY-2 (Bright Yellow 2) cultures performed in a hollow-fibre bioreactor has resulted in 1.1 time k L a increase, as well as up to 5 times higher biomass yield in comparison to the culture system utilising atmospheric air as oxygenation medium (Pilarek et al, 2009). A hollow-fibre bioreactor has been also utilised for monoclonal antibody (mAb) production in cultures of mouse 3C11 hybridoma cells (Shi et al, 1998). The application of aerated Oxyfluor emulsion as an oxygenation agent has resulted in a 78 % increase in yield of IG 1 -type mAbs.…”

Section: Pfc-mediated Oxygen Transfer Intensificationmentioning

confidence: 99%

Liquid Perfluorochemicals as Flexible and Efficient Gas Carriers Applied in Bioprocess Engineering: An Updated Overview and Future Prospects

Pilarek¹

2014

Chemical and Process Engineering

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Fully synthetic, biochemically inert and water-immiscible liquid perfluorochemicals (PFCs) are recognised as flexible liquid carriers/scavengers of gaseous compounds (respiratory gases mainly, i.e. O 2 and CO 2 ) and increasingly applied in bioprocess engineering. A range of unmatched physicochemical properties of liquid PFCs, i.e. outstanding chemo-and thermostability, extremely low surface tension, simultaneous hydro-and lipophobicity, which result from carbon chain substitution with fluorine atoms (the most electronegative chemical element) and the presence of intramolecular C-F bonds (the strongest single bond known in organic chemistry) have been described in detail. Exceptional propensity to solubility of respiratory gases in liquid perfluorinated compounds has been widely discussed. Advantages and disadvantages of bioprocess applications of liquid PFCs in the form of a pure PFC as well as in an emulsified form have been pointed out. A liquid PFC-mediated mass transfer intensification in various types of microbial, plant cell and animal cell culture systems: from miniaturised microlitre-scale cultures, via biomaterial-based scaffolds containing culture systems, to litre-scale bioreactors, has been reviewed and elaborated on bearing in mind the benefits of bioprocesses.

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“…Therefore, the LEH of the existing design cannot be used as an oxygen carrier to precursor liver tissue where we need to grow hepatocyte progenitors. Hbbased oxygen carrier is ideal, but as shown in the study of Shi et al who observed strong toxicity of free Hb when it is directly added to cell culture with over 1% concentrations [112].…”

Section: Hemoglobin (Hb)-based Oxygen Carriersmentioning

confidence: 99%

Toward engineering of vascularized three-dimensional liver tissue equivalents possessing a clinically significant mass

Sakai

Huang

Hanada

et al. 2010

Biochemical Engineering Journal

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The use of oxygen carriers for increasing the production of monoclonal antibodies from hollow fibre bioreactors

Cited by 12 publications

References 15 publications

Hemoglobin Regulates the Metabolic, Synthetic, Detoxification, and Biotransformation Functions of Hepatoma Cells Cultured in a Hollow Fiber Bioreactor

Hemoglobin Regulates the Metabolic, Synthetic, Detoxification, and Biotransformation Functions of Hepatoma Cells Cultured in a Hollow Fiber Bioreactor

Liquid Perfluorochemicals as Flexible and Efficient Gas Carriers Applied in Bioprocess Engineering: An Updated Overview and Future Prospects

Toward engineering of vascularized three-dimensional liver tissue equivalents possessing a clinically significant mass

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