The use of nucleosides and arginine as alternative energy sources by coagulase-negative staphylococci in view of meat fermentation

Janssens, Maarten; Mijnsbrugge, A. Van der; Mainar, María Sánchez; Balzarini, Tom; Vuyst, Luc De; Leroy, Frédéric

doi:10.1016/j.fm.2013.11.004

Cited by 28 publications

(26 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The capacity to utilize specific substrates other than carbohydrates may partially explain differences in competitiveness between CNS species, possibly even on the strain level. Arginine forms an interesting candidate substrate, due to its relative prevalence in meat and its potential to yield ATP and improve acid stress tolerance through the ADI pathway (9,41). From the present study, it is clear that ADI activity is very common in CNS, but not in all species, and even displays heterogeneity at the strain level.…”

Section: Discussionmentioning

confidence: 75%

“…Although not all-explanatory, a functional ADI pathway may nevertheless represent a competitive benefit to certain CNS species. This may, for instance, be the case in acidified meat matrices, as suggested for S. carnosus (9). In addition to the native arc operon, competitiveness can also be affected by the presence of a second gene cluster encoding a complete ADI pathway, such as the arginine catabolic mobile element (ACME).…”

Section: Discussionmentioning

confidence: 99%

“…Genomic DNA of all 86 CNS strains was isolated from respective overnight cultures in BHI medium at 30°C. Cell pellets were obtained by centrifugation of 1 ml of these cultures (21, PCR assays were performed using a T300 thermocycler (Biometra GmbH, Göttingen, Germany), containing 200 ng of genomic DNA, 200 M each deoxynucleoside triphosphate (dNTP), 20 pmol of each primer, 5 l of 10ϫ PCR buffer, 1.25 U of Taq DNA polymerase (Roche, Basel, Switzerland), and sterile ultrapure water in a final total volume of 50 l. For the PCR assay targeting the arcA gene, the conditions were as previously described (9). For the other genotypic screening experiments, the following conditions were used for the PCR assays with the different primer pairs: initial denaturation at 94°C for 120 s, 30 cycles of denaturation at 94°C for 30 s, annealing at 55°C (primer pair NOS-2), 50°C (primer pairs NOS-1 and NOS-4), or 45°C (primer pair NOS-3 and primer pairs for the genes encoding arginase and OTC) for 60 s, and extension at 72°C for 60 s, with a final extension at 72°C for 420 s. The optimum annealing temperatures for the PCR conditions were determined by performing a gradient PCR with a TProfessional basic gradient thermocycler (Biometra GmbH).…”

Section: Methodsmentioning

confidence: 99%

“…In fermented meats, for instance, carbohydrates are naturally scarce, whereas the fermentable sugars that are initially added to the sausage batter to boost fermentation are rapidly scavenged by the lactic acid bacteria. As a result, CNS must shift from common carbohydrates toward the use of alternative endogenous energy sources, such as nucleosides or arginine (9), a capacity that has also been demonstrated for meat-associated L. sakei strains (10)(11)(12).…”

mentioning

confidence: 99%

“…The arginine deiminase (ADI) pathway has been reported as a common route for arginine degradation in bacteria, including CNS (15), although variability on both species and strain levels exists (9). The ADI pathway leads to the production of extra ATP, improved survival under acid stress conditions via ammonia production, and the use of intermediate carbamoyl phosphate for pyrimidine biosynthesis.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Coagulase-Negative Staphylococci Favor Conversion of Arginine into Ornithine despite a Widespread Genetic Potential for Nitric Oxide Synthase Activity

Mainar

Weckx

Leroy

2014

Appl Environ Microbiol

View full text Add to dashboard Cite

Within ecosystems that are poor in carbohydrates, alternative substrates such as arginine may be of importance to coagulasenegative staphylococci (CNS). However, the versatility of arginine conversion in CNS remains largely uncharted. Therefore, a set of 86 strains belonging to 17 CNS species was screened for arginine deiminase (ADI), arginase, and nitric oxide synthase (NOS) activities, in view of their ecological relevance. In fermented meats, for instance, ADI could improve bacterial competitiveness, whereas NOS may serve as an alternative nitrosomyoglobin generator to nitrate and nitrite curing. About 80% of the strains were able to convert arginine, but considerable inter-and intraspecies heterogeneity regarding the extent and mechanism of conversion was found. Overall, ADI was the most commonly employed pathway, resulting in mixtures of ornithine and small amounts of citrulline. Under aerobic conditions, which are more relevant for skin-associated CNS communities, several strains shifted toward arginase activity, leading to the production of ornithine and urea. The obtained data indeed suggest that arginase occurs relatively more in CNS isolates from a dairy environment, whereas ADI seems to be more abundant in strains from a fermented meat background. With some exceptions, a reasonable match between phenotypic ADI and arginase activity and the presence of the encoding genes (arcA and arg) was found. With respect to the NOS pathway, however, only one strain (Staphylococcus haemolyticus G110) displayed phenotypic NOS-like activity under aerobic conditions, despite a wide prevalence of the NOS-encoding gene (nos) among CNS. Hence, the group of CNS displays a strain-and condition-dependent toolbox of arginineconverting mechanisms with potential implications for competitiveness and functionality.

show abstract

Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Coagulase-Negative Staphylococci Favor Conversion of Arginine into Ornithine despite a Widespread Genetic Potential for Nitric Oxide Synthase Activity

Mainar

Weckx

Leroy

2014

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

Characterization of microbial community composition and pathogens risk assessment in typical Italian-style salami by high-throughput sequencing technology

Wang

Ren

Zhan

2017

Food Sci Biotechnol

View full text Add to dashboard Cite

The structure of microbial communities in a typical Italian-style salami, including bacterial and fungal diversity, was investigated by high-throughput sequencing technology. A total of 6 phyla, 7 classes, 19 orders, 20 families and 28 genera were obtained from 16S rDNA sequences, and a total of 2 phyla, 4 classes, 4 orders, 5 families, 10 genera and 12 Species were obtained from 18S rDNA sequences. The core microbiota was composed of , representing up to 97.52% of the total 16S rRNA, and, accounting for 99.74% of the total classified 18S rRNA. and were detected with a quite low proportion of 1.71 and 0.007%, respectively. This study contributes to the knowledge of the microbial diversity involved in salami and presents high-throughput sequencing as a useful tool to evaluate microbial diversity and monitor the food-borne pathogens in fermented sausage.

show abstract

Impact of collection conditions on the metabolite content of human urine samples as analyzed by liquid chromatography coupled to mass spectrometry and nuclear magnetic resonance spectroscopy

et al. 2014

View full text Add to dashboard Cite

There is a lack of comprehensive studies documenting the impact of sample collection conditions on metabolic composition of human urine. To address this issue, two experiments were performed at a 3-month interval, in which midstream urine samples from healthy individuals were collected, pooled, divided into several aliquots and kept under specific conditions (room temperature, 4 °C, with or without preservative) up to 72 h before storage at −80 °C. Samples were analyzed by high-performance liquid chromatography coupled to high-resolution mass spectrometry and bacterial contamination was monitored by turbidimetry. Multivariate analyses showed that urinary metabolic fingerprints were affected by the presence of preservatives and also by storage at room temperature from 24 to 72 h, whereas no change was observed for urine samples stored at 4 °C over a 72-h period. Investigations were then focused on 280 metabolites previously identified in urine: 19 of them were impacted by the kind of sample collection protocol in both experiments, including 12 metabolites affected by bacterial contamination and 7 exhibiting poor chemical stability. Finally, our results emphasize that the use of preservative prevents bacterial overgrowth, but does not avoid metabolite instability in solution, whereas storage at 4 °C inhibits bacterial overgrowth at least over a 72-h period and slows the chemical degradation process. Consequently, and for further LC/MS analyses, human urine samples should be kept at 4 °C if their collection is performed over 24 h.Electronic supplementary materialThe online version of this article (doi:10.1007/s11306-014-0764-5) contains supplementary material, which is available to authorized users.

show abstract

The use of nucleosides and arginine as alternative energy sources by coagulase-negative staphylococci in view of meat fermentation

Cited by 28 publications

References 43 publications

Coagulase-Negative Staphylococci Favor Conversion of Arginine into Ornithine despite a Widespread Genetic Potential for Nitric Oxide Synthase Activity

Coagulase-Negative Staphylococci Favor Conversion of Arginine into Ornithine despite a Widespread Genetic Potential for Nitric Oxide Synthase Activity

Characterization of microbial community composition and pathogens risk assessment in typical Italian-style salami by high-throughput sequencing technology

Impact of collection conditions on the metabolite content of human urine samples as analyzed by liquid chromatography coupled to mass spectrometry and nuclear magnetic resonance spectroscopy

Contact Info

Product

Resources

About