The use of newly isolated <i>Streptomyces</i> strain TN258 as potential biocontrol agent of potato tubers leak caused by <i>Pythium ultimum</i>

Sellem, Imen; Triki, Mohamed Ali; Elleuch, Lobna; Cheffi, Manel; Chakchouk, Ahlem; Smaoui, Slim; Mellouli, Lotfi

doi:10.1002/jobm.201600604

Cited by 18 publications

(8 citation statements)

References 27 publications

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“…Many species of bacteria, especially Bacillus, are known as biological control agents that inhibit several phytopathogenic fungi due to the production of cell wall-degrading enzymes and other antifungal metabolic products[19–21]. Many Bacillus strains antagonistic to fungi have been isolated from soil[10, 22–24].…”

Section: Discussionmentioning

confidence: 99%

The isolation of the antagonistic strain Bacillus australimaris CQ07 and the exploration of the pathogenic inhibition mechanism of Magnaporthe oryzae

Chen

Zhao

et al. 2019

PLoS ONE

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Biological control as a promising method to combat plant disease has gained public attention in recent years. In the present study, we isolated 12 strains resistant to Magnaporthe oryzae from western Sichuan subalpine soil. Among them, CQ07 exhibited remarkable activity against M . oryzae . The result of 16S rRNA sequence analysis revealed that CQ07 is approximately 99% similar to Bacillus australimaris . The sterilized culture filtrate of CQ07 inhibited the growth of M . oryzae , which motivated us to deduce the influence of CQ07 on the pathogenicity of M . oryzae . As shown by experimentation, sterilized culture filtrate (10 μl/ml) of CQ07 can delay and even suppress the germination of conidia and prevent the formation of appressorium in vitro and in vivo. In addition, by simulative field tests, the spraying of conidia suspension diluted with sterilized culture filtrate of CQ07 reduced infection of rice blast. To better control rice blasts, understanding the infection mechanism of M . oryzae and inhibiting the mechanism of the antagonistic strain is of great importance.

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Section: Discussionmentioning

confidence: 99%

The isolation of the antagonistic strain Bacillus australimaris CQ07 and the exploration of the pathogenic inhibition mechanism of Magnaporthe oryzae

Chen

Zhao

et al. 2019

PLoS ONE

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“…Plant fungal diseases are difficult to control, which makes it possible to lead to huge economic losses. Thus, strategies to ensure that plants avoid to fungal infection should be developed, especially by the development of biological control methods [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation and evaluation of endophytic Bacillus tequilensis GYLH001 with potential application for biological control of Magnaporthe oryzae

Guan

Dong

et al. 2018

PLoS ONE

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Biological control is a promising measure in the control of plant disease. In the present study, we isolated 13 endophytic strains from Angelica dahurica. Among them, an endophytic strain which was named GYLH001 exhibited remarkable activity against Magnaporthe oryzae. 16S rRNA sequence analysis, biochemical and physiological proved that it is Bacillus tequilensis. The sterilized culture filtrate of GYLH001 can inhibit the growth of M.oryzae, which suggests the presence of secondary metabolites. Proved by experiment, GYLH001 can produce cellulase, protease, gelatinase, indole-3-acetic acid and 1-amino-cyclopropane-1-carboxylate deaminase. In addition, the temperature experiment showed that secondary metabolites produced by GYLH001 had good thermal stability. They can remain activity even heated at 100°C for 30 min. They also had good acid-resistance in heavily acidic condition. But under alkaline condition, the antifungal effect decreased significantly. By simulative field tests, the spraying of GYLH001 spore solution could prevent and treat rice blast. Through continuous separation and purification of sterilized culture filtrate and identification by mass spectrometry, the molecular weight of an active substance is 364.26. In the control of rice blast, B. tequilensis GYLH001 has potential as a biological control agent in agriculture.

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“…The inoculum of M. phaseolina and R. solani was prepared by seeding suspensions of mycelial fragments of each isolate on Potato Dextrose Agar (PDA; BioCen, Bejucal, Mayabeque, Cuba) at 28 °C for three days in darkness. In vitro dual culture assays were conducted in 9.0 cm in diameter Petri dishes with PDA [ 37 ]. To this end, a 7.0 mm in diameter mycelial plug of the pathogen was placed at one end of the plate, and another 7.0 mm in diameter mycelial plug of the actinobacterial strain was plated at 50.0 mm apart at the opposite end.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Actinobacterial Strains as Potential Biocontrol Agents against Macrophomina phaseolina and Rhizoctonia solani, the Main Soil-Borne Pathogens of Phaseolus vulgaris in Cuba

Díaz-Díaz

Bernal-Cabrera

Trapero

et al. 2022

Plants

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Macrophomina phaseolina and Rhizoctonia solani are considered two major soil-borne pathogens of Phaseolus vulgaris in Cuba. Their management is difficult, not only due to their intrinsic biology as soil-borne pathogens, but also because the lack of active ingredients available against these pathogens. Actinobacteria, a heterogeneous bacterial group traditionally known as actinomycetes have been reported as promising biological control agents (BCAs) in crop protection. Thus, the main objective of this study was to evaluate the effectiveness of 60 actinobacterial strains as BCAs against M. phaseolina and R. solani in vitro by dual culture assays. The most effective strains were characterized according to their cellulolytic, chitinolytic and proteolytic extracellular enzymatic activity, as well as by their morphological and biochemical characters in vitro. Forty and 25 out of the 60 actinobacteria strains inhibited the mycelial growth of M. phaseolina and R. solani, respectively, and 18 of them showed a common effect against both pathogens. Significant differences were observed on their enzymatic and biochemical activity. The morphological and biochemical characters allow us to identify all our strains as species belonging to the genus Streptomyces. Streptomyces strains CBQ-EA-2 and CBQ-B-8 showed the highest effectiveness in vitro. Finally, the effect of seed treatments by both strains was also evaluated against M. phaseolina and R. solani infections in P. vulgaris cv. Quivicán seedlings. Treatments combining the two Streptomyces strains (CBQ-EA-2 + CBQ-B-8) were able to reduce significantly the disease severity for both pathogen infections in comparison with the non-treated and inoculated control. Moreover, they showed similar effect than that observed for Trichoderma harzianum A-34 and with Celest® Top 312 FS (Syngenta®; Basilea, Switzerland) treatments, which were included for comparative purposes.

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The use of newly isolated Streptomyces strain TN258 as potential biocontrol agent of potato tubers leak caused by Pythium ultimum

Cited by 18 publications

References 27 publications

The isolation of the antagonistic strain Bacillus australimaris CQ07 and the exploration of the pathogenic inhibition mechanism of Magnaporthe oryzae

The isolation of the antagonistic strain Bacillus australimaris CQ07 and the exploration of the pathogenic inhibition mechanism of Magnaporthe oryzae

Isolation and evaluation of endophytic Bacillus tequilensis GYLH001 with potential application for biological control of Magnaporthe oryzae

Characterization of Actinobacterial Strains as Potential Biocontrol Agents against Macrophomina phaseolina and Rhizoctonia solani, the Main Soil-Borne Pathogens of Phaseolus vulgaris in Cuba

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