Isolation and evaluation of endophytic Bacillus tequilensis GYLH001 with potential application for biological control of Magnaporthe oryzae

Abstract

Biological control is a promising measure in the control of plant disease. In the present study, we isolated 13 endophytic strains from Angelica dahurica. Among them, an endophytic strain which was named GYLH001 exhibited remarkable activity against Magnaporthe oryzae. 16S rRNA sequence analysis, biochemical and physiological proved that it is Bacillus tequilensis. The sterilized culture filtrate of GYLH001 can inhibit the growth of M.oryzae, which suggests the presence of secondary metabolites. Proved by experiment, GYLH001 can produce cellulase, protease, gelatinase, indole-3-acetic acid and 1-amino-cyclopropane-1-carboxylate deaminase. In addition, the temperature experiment showed that secondary metabolites produced by GYLH001 had good thermal stability. They can remain activity even heated at 100°C for 30 min. They also had good acid-resistance in heavily acidic condition. But under alkaline condition, the antifungal effect decreased significantly. By simulative field tests, the spraying of GYLH001 spore solution could prevent and treat rice blast. Through continuous separation and purification of sterilized culture filtrate and identification by mass spectrometry, the molecular weight of an active substance is 364.26. In the control of rice blast, B. tequilensis GYLH001 has potential as a biological control agent in agriculture.

Culturable Bacterial Community on Leaves of Assam Tea (Camellia sinensis var. assamica) in Thailand and Human Probiotic Potential of Isolated Bacillus spp.

“…have been widely found in various environments. Some species of Bacillus are pathogenic (e.g., B. cereus ), probiotics (e.g., B. clausii , B. subtilis , B. licheniformis ), endophytic (with the ability to produce the plant hormone indole-3-acetic acid or IAA; e.g., B. altitudinis , B. paramycoides , B. tequilensis ), and antimicrobial (e.g., B. niacin , B. mobilis ) [ 52 , 53 , 54 , 55 , 56 ]. Staphylococcus spp., Macrococcus spp., and Micrococcus spp.…”

Bacillus velezensis BM21, a potential and efficient biocontrol agent in control of corn stalk rot caused by Fusarium graminearum

“…The seedlings were watered daily using overhead irrigation to maintain soil moisture. Disease incidence was evaluated 20 days after sowing and was quantified as the percentage of diseased plants (Li et al 2018). Disease reduction (%) was calculated as disease incidence of untreated control−disease incidence of the treatment disease incidence of the untreated control  100%…”

“…Biological control is a promising approach in the control of plant diseases (Li et al 2018). Plants are able to recruit protective microorganisms and enhance microbial activity to suppress pathogens in the rhizosphere (Berendsen et al 2012).…”

Identification and Characterization of Bacterial Community Associated with the Chewed Feeding Waste of Red Palm Weevil in Infested Date Palm Trees

“…nov., showed close realation with Bacillus subtilis [35]. In another report, B. tequilensis GYLH001 regarded as endophytic bacteria and was used as biocontrol agent of fungus Magnaporthe oryzae causing rice blast [36]. Moreover, Bacillus altitudinis P-10 worked as potential bioprotectant against Xanthomonas oryzae pv.…”