“…Tere are three types of cofee in the market: green, roasted, and processed cofee [20], all are susceptible to being adulterated with the by-products of cofee or other plant materials, such as cofee husks [21,22], waste cofee grounds [23], brown sugar [24], acai [25], cheaper grains (wheat [26], rice [27], rye [28], barley [2,29,30], corn [26,[31][32][33], soybean [32]), and chicory [30,34]. Besides, intentionally mislabeling lower-valued beans as their higher-valued counterparts and blending different species or origins of beans are other possible forms of fraud [35,36]. In response to the increasing public concerns of fraudulent practices about cofee, the consumers and actors of the food industry sector, including the related government agencies and food companies, demand the methods for the characterization of cofee to be adequately reliable, accurate, high-throughput, low-cost, as well as easy-to-operate with minimal human intervention [37].…”