The Use of Multiple Antigen Peptides in the Analysis and Induction of Protective Immune Responses against Infectious Diseases

Eh, Nardin; Oliveira, Gerlon de Almeida Ribeiro; Jm, Calvo-Calle; Rs, Nussenzweig

doi:10.1016/s0065-2776(08)60585-4

Cited by 54 publications

(33 citation statements)

References 110 publications

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“…However, a more sensitive measure of induced antibodies may reveal responses that we have been unable to detect preboost. Branched multiple-antigen peptides or linking linear peptides to a carrier molecule can provide a more sensitive detection method than linear peptide ELISA (26).…”

Section: Discussionmentioning

confidence: 99%

Combination of Protein and Viral Vaccines Induces Potent Cellular and Humoral Immune Responses and Enhanced Protection from Murine Malaria Challenge

Hutchings

Birkett²,

Moore

et al. 2007

Infect Immun

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The search for an efficacious vaccine against malaria is ongoing, and it is now widely believed that to confer protection a vaccine must induce very strong cellular and humoral immunity concurrently. We studied the immune response in mice immunized with the recombinant viral vaccines fowlpox strain FP9 and modified virus Ankara (MVA), a protein vaccine (CV-1866), or a combination of the two; all vaccines express parts of the same preerythrocytic malaria antigen, the Plasmodium berghei circumsporozoite protein (CSP). Mice were then challenged with P. berghei sporozoites to determine the protective efficacies of different vaccine regimens. Two immunizations with the protein vaccine CV-1866, based on the hepatitis B core antigen particle, induced strong humoral immunity to the repeat region of CSP that was weakly protective against sporozoite challenge. Prime-boost with the viral vector vaccines, FP9 followed by MVA, induced strong T-cell immunity to the CD8 ؉ epitope Pb9 and partially protected animals from challenge. Physically mixing CV-1866 with FP9 or MVA and then immunizing with the resultant combinations in a prime-boost regimen induced both cellular and humoral immunity and afforded substantially higher levels of protection (combination, 90%) than either vaccine alone (CV-1866, 12%; FP9/MVA, 37%). For diseases such as malaria in which different potent immune responses are required to protect against different stages, using combinations of partially effective vaccines may offer a more rapid route to achieving deployable levels of efficacy than individual vaccine strategies.

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Section: Discussionmentioning

confidence: 99%

Combination of Protein and Viral Vaccines Induces Potent Cellular and Humoral Immune Responses and Enhanced Protection from Murine Malaria Challenge

Hutchings

Birkett²,

Moore

et al. 2007

Infect Immun

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“…The IF assay was performed on air-dried P. falciparum sporozoites as described (1). For the ELISA, we used a tetrabranched multiple antigen peptide based on the repeat of the P. falciparum CS protein [(NANP) 3 ] 4 , designated (B) 4 , as a highly sensitive antigen for antibody detection (14). The titers correspond to the highest serum dilution giving an OD Ͼ2 ϫ the mean OD obtained with normal BALB͞c sera.…”

Section: Methodsmentioning

confidence: 99%

Recombinant viruses expressing a human malaria antigen can elicit potentially protective immune CD8⁺responses in mice

Miyahira

Garcı́a-Sastre

Rodrı́guez

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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“…In the last decade, several approaches have been pursued to develop a vaccine against S. pyogenes. The M protein, a major virulence factor of S. pyogenes, constitutes the bestcharacterized vaccine candidate (2,4,17). However, M protein mainly triggers serotype-specific immunity and may lead to cross-reaction with host tissues.…”

mentioning

confidence: 99%

“…However, peptides are usually poorly immunogenic and need to be administered with depot-forming adjuvants (19,30) or bacterial toxins or their derivatives (2,24). They can also be generated using approaches in which multiple copies of the epitopes are displayed (17,31).…”

mentioning

confidence: 99%

Identification of B- and T-Cell Epitopes within the Fibronectin-Binding Domain of the SfbI Protein ofStreptococcus pyogenes

et al. 2003

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The fibronectin-binding repeats of the SfbI protein of Streptococcus pyogenes constitute the minimal domain able to confer protection against lethal infection. We investigated the presence of B-and T-cell epitopes within this region in congenic mice. One linear B-cell epitope was recognized by BALB/b and BALB/k mice, whereas two epitopes were found in BALB/c animals. A unique T-cell epitope was recognized by all three mouse strains. All identified epitopes clustered in a 30-amino-acid fragment. These results suggest that this polypeptide may be suitable for incorporation into a polyepitope-based vaccine formulation against S. pyogenes.The human pathogen Streptococcus pyogenes can cause localized infections resulting in noninvasive diseases like pharyngitis, impetigo, or scarlet fever. These infections can lead to severe poststreptococcal diseases, such as rheumatic fever and acute poststreptococcal glomerulonephritis. In addition, S. pyogenes can cause highly invasive diseases, such as sepsis, necrotizing fasciitis, and toxic shock-like syndrome (3, 25, 32). The incidence of S. pyogenes infections and their sequelae, as well as the emergence of strains that are resistant to macrolides (e.g., erythromycin), has been steadily increasing (7,11,13,18). In the last decade, several approaches have been pursued to develop a vaccine against S. pyogenes. The M protein, a major virulence factor of S. pyogenes, constitutes the bestcharacterized vaccine candidate (2, 4, 17). However, M protein mainly triggers serotype-specific immunity and may lead to cross-reaction with host tissues. In the last few years the C5a peptidase, a surface-bound peptidase that cleaves C5a (10), and the extracellular cysteine protease, which cleaves fibronectin and converts interleukin-1␤ precursor to biologically active interleukin-1␤ (12), have been proposed as candidate antigens. The use of peptides encompassing conserved B-and T-cell epitopes of the M protein constitutes a new approach to prevent diseases caused by different M serotypes and the potential side effects resulting from immune cross-recognition (1,4,5,6,20). However, peptides are usually poorly immunogenic and need to be administered with depot-forming adjuvants (19,30) or bacterial toxins or their derivatives (2, 24). They can also be generated using approaches in which multiple copies of the epitopes are displayed (17, 31).It has been shown that intranasal vaccination with the fibronectin-binding protein I (SfbI) of S. pyogenes stimulates humoral and cellular immune responses, which are able to protect against lethal challenge with S. pyogenes (9, 22). The SfbI protein plays a central role in the virulence process; is highly conserved, surface located, and expressed by a large number of clinical isolates from different serotypes (approximately 73%); and does not lead to cross-reactivity with host tissues (16,(26)(27)(28)(29). Additional studies have identified the domain encompassing the fibronectin-binding repeats (148 amino acids) as the minimal fragment able to confer protect...

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The Use of Multiple Antigen Peptides in the Analysis and Induction of Protective Immune Responses against Infectious Diseases

Cited by 54 publications

References 110 publications

Combination of Protein and Viral Vaccines Induces Potent Cellular and Humoral Immune Responses and Enhanced Protection from Murine Malaria Challenge

Combination of Protein and Viral Vaccines Induces Potent Cellular and Humoral Immune Responses and Enhanced Protection from Murine Malaria Challenge

Recombinant viruses expressing a human malaria antigen can elicit potentially protective immune CD8⁺responses in mice

Identification of B- and T-Cell Epitopes within the Fibronectin-Binding Domain of the SfbI Protein ofStreptococcus pyogenes

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The Use of Multiple Antigen Peptides in the Analysis and Induction of Protective Immune Responses against Infectious Diseases

Cited by 54 publications

References 110 publications

Combination of Protein and Viral Vaccines Induces Potent Cellular and Humoral Immune Responses and Enhanced Protection from Murine Malaria Challenge

Combination of Protein and Viral Vaccines Induces Potent Cellular and Humoral Immune Responses and Enhanced Protection from Murine Malaria Challenge

Recombinant viruses expressing a human malaria antigen can elicit potentially protective immune CD8+responses in mice

Identification of B- and T-Cell Epitopes within the Fibronectin-Binding Domain of the SfbI Protein ofStreptococcus pyogenes

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Recombinant viruses expressing a human malaria antigen can elicit potentially protective immune CD8⁺responses in mice