Recombinant viruses expressing a human malaria antigen can elicit potentially protective immune CD8<sup>+</sup>responses in mice

Miyahira, Yasushi; Garcı́a-Sastre, Adolfo; Rodrı́guez, Dolores; Rodríguez, Juan; Murata, Kenichiro; Tsuji, Moriya; Palese, Peter; Esteban, Mariano; Zavala, Fidel; Nussenzweig, Ruth S.

doi:10.1073/pnas.95.7.3954

Cited by 80 publications

(48 citation statements)

References 22 publications

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“…Malaria, for example, is gradually controlled by the immune response in those that survive, though the parasite may persist and the process can take years. Providing a "jump start" for this process with appropriate vectors carrying CD8 ϩ T-cell epitopes could thus speed recovery (26). The relatively low growth rates of tumor cells (3,18,39) when compared with those of viruses also favor control by primed CD8 ϩ T cells.…”

Section: Discussionmentioning

confidence: 99%

Profound Protection against Respiratory Challenge with a Lethal H7N7 Influenza A Virus by Increasing the Magnitude of CD8⁺T-Cell Memory

Christensen

Doherty

Branum

et al. 2000

J Virol

111

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The recall of CD8؉ T-cell memory established by infecting H-2 b mice with an H1N1 influenza A virus provided a measure of protection against an extremely virulent H7N7 virus. The numbers of CD8 ؉ effector and memory T cells specific for the shared, immunodominant D b NP 366 epitope were greatly increased subsequent to the H7N7 challenge, and though lung titers remained as high as those in naive controls for 5 days or more, the virus was cleared more rapidly. Expanding the CD8؉ memory T-cell pool (<0.5 to >10%) by sequential priming with two different influenza A viruses (H3N23H1N1) gave much better protection. Though the H7N7 virus initially grew to equivalent titers in the lungs of naive and double-primed mice, the replicative phase was substantially controlled within 3 days. This tertiary H7N7 challenge caused little increase in the magnitude of the CD8 ؉ D b NP 366 ؉ T-cell pool, and only a portion of the memory population in the lymphoid tissue could be shown to proliferate. The great majority of the CD8 ؉ D b NP 366 ؉ set that localized to the infected respiratory tract had, however, cycled at least once, though recent cell division was shown not to be a prerequisite for T-cell extravasation. The selective induction of CD8 ؉ T-cell memory can thus greatly limit the damage caused by a virulent influenza A virus, with the extent of protection being directly related to the number of available responders. Furthermore, a large pool of CD8 ؉ memory T cells may be only partially utilized to deal with a potentially lethal influenza infection.

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Section: Discussionmentioning

confidence: 99%

Profound Protection against Respiratory Challenge with a Lethal H7N7 Influenza A Virus by Increasing the Magnitude of CD8⁺T-Cell Memory

Christensen

Doherty

Branum

et al. 2000

J Virol

111

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“…No protective immunity could be observed in this case (Li et al 1993). These findings were recently extended to other malaria CD8 epitopes expressed in the CS protein of P. falciparum, a human malaria parasite (Miyahira et al 1998). Based on these observations, several authors have explored the heterologous prime-boost immunization to increase the CD8 immune response.…”

Section: Malaria Liver Stagesmentioning

confidence: 99%

Importance of CD8 T cell-mediated immune response during intracellular parasitic infections and its implications for the development of effective vaccines

Rodrigues

Boscardin

Vasconcelos

et al. 2003

An. Acad. Bras. Ciênc.

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Obligatory intracellular parasites such as Plasmodium sp, Trypanosoma cruzi, Toxoplasma gondii and Leishmania sp are responsible for the infection of hundreds of millions of individuals every year. These parasites can deliver antigens to the host cell cytoplasm that are presented through MHC class I molecules to protective CD8 T cells. The in vivo priming conditions of specific CD8 T cells during natural infection are largely unknown and remain as an area that has been poorly explored. The antiparasitic mechanisms mediated by CD8 T cells include both interferon-γ -dependent and -independent pathways. The fact that CD8 T cells are potent inhibitors of parasitic development prompted many investigators to explore whether induction of these T cells can be a feasible strategy for the development of effective subunit vaccines against these parasitic diseases. Studies performed on experimental models supported the hypothesis that CD8 T cells induced by recombinant viral vectors or DNA vaccines could serve as the basis for human vaccination. Regimens of immunization consisting of two different vectors (heterologous prime-boost) are much more efficient in terms of expansion of protective CD8 T lymphocytes than immunization with a single vector. The results obtained using experimental models have led to clinical vaccination trials that are currently underway.

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“…Therefore, several Ags that are expressed by Plasmodium are used for immunization against malaria (1)(2)(3)(4)(5)(6). These trials examined the concept of the induction of anti-malaria Abs produced by conventional B cells and the induction of cellular immunity mediated by conventional T cells.…”

mentioning

confidence: 99%

Essential Role of Extrathymic T Cells in Protection Against Malaria

Mannoor

Halder

Morshed

et al. 2002

The Journal of Immunology

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Athymic nude mice carry neither conventional T cells nor NKT cells of thymic origin. However, NK1.1−TCRint cells are present in the liver and other immune organs of athymic mice, because these lymphocyte subsets are truly of extrathymic origin. In this study, we examined whether extrathymic T cells had the capability to protect mice from malarial infection. Although B6-nu/nu mice were more sensitive to malaria than control B6 mice, these athymic mice were able to survive malaria when a reduced number of parasitized erythrocytes (5 × 103 per mouse) were injected. At the fulminant stage, lymphocytosis occurred in the liver and the major expanding lymphocytes were NK1.1−TCRint cells (IL-2Rβ+TCRαβ+). Unconventional CD8+ NKT cells (Vα14−) also appeared. Similar to the case of B6 mice, autoantibodies (IgM type) against denatured DNA appeared during malarial infection. Immune lymphocytes isolated from the liver of athymic mice which had recovered from malaria were capable of protecting irradiated euthymic and athymic mice from malaria when cell transfer experiments were conducted. In conjunction with the previous results in euthymic mice, the present results in athymic mice suggest that the major lymphocyte subsets associated with protection against malaria might be extrathymic T cells.

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Recombinant viruses expressing a human malaria antigen can elicit potentially protective immune CD8⁺responses in mice

Cited by 80 publications

References 22 publications

Profound Protection against Respiratory Challenge with a Lethal H7N7 Influenza A Virus by Increasing the Magnitude of CD8⁺T-Cell Memory

Profound Protection against Respiratory Challenge with a Lethal H7N7 Influenza A Virus by Increasing the Magnitude of CD8⁺T-Cell Memory

Importance of CD8 T cell-mediated immune response during intracellular parasitic infections and its implications for the development of effective vaccines

Essential Role of Extrathymic T Cells in Protection Against Malaria

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Recombinant viruses expressing a human malaria antigen can elicit potentially protective immune CD8+responses in mice

Cited by 80 publications

References 22 publications

Profound Protection against Respiratory Challenge with a Lethal H7N7 Influenza A Virus by Increasing the Magnitude of CD8+T-Cell Memory

Profound Protection against Respiratory Challenge with a Lethal H7N7 Influenza A Virus by Increasing the Magnitude of CD8+T-Cell Memory

Importance of CD8 T cell-mediated immune response during intracellular parasitic infections and its implications for the development of effective vaccines

Essential Role of Extrathymic T Cells in Protection Against Malaria

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Product

Resources

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Recombinant viruses expressing a human malaria antigen can elicit potentially protective immune CD8⁺responses in mice

Profound Protection against Respiratory Challenge with a Lethal H7N7 Influenza A Virus by Increasing the Magnitude of CD8⁺T-Cell Memory

Profound Protection against Respiratory Challenge with a Lethal H7N7 Influenza A Virus by Increasing the Magnitude of CD8⁺T-Cell Memory