The use of mitochondrial cytochrome oxidase I gene (COI) to differentiate two UK blowfly species – Calliphora vicina and Calliphora vomitoria

Molecular Identification of Forensically Important Indian Species of Flesh Flies (Diptera: Sarcophagidae) by Using COI Gene of Mitochondrial DNA

“…Most forensic entomological clues are reliant on unquestionable species identification. This estimation is founded upon the time taken for insects evolving on a corpse to come to the stage present when the body is discovered [7,8]. Insect species have distinct developmental lifecycle timings and thus to utilize the correct developmental data, species need to be accurately recognized.…”

“…Insect species have distinct developmental lifecycle timings and thus to utilize the correct developmental data, species need to be accurately recognized. This can be done on the basis of morphological differences [7,8].…”

“…The larval types and eggs of numerous forensically significant dipteran species are tough to distinguish as their morphological differences may be obscured or awfully maintained [8,9]. Definitive identification may be achieved by rearing larvae to adults but this can be time consuming and need the larvae to be assembled, reside and kept in situation optimal for proceeded development [8,9].…”

“…Definitive identification may be achieved by rearing larvae to adults but this can be time consuming and need the larvae to be assembled, reside and kept in situation optimal for proceeded development [8,9]. The members of family Sarcophagidae or flesh flies (Diptera) comprise over 2,500 species in over 100 genera globally, with numerous species being carrion breeders and initial corpse colonizers [10,11].…”

“…The use of DNA methods can not only supplement the traditional morphological methods but may furthermore give information on population substructures [8,15]. This identification can be carried out on any lifecycle stage without further rearing and on dead, maintained or reside trials.…”