Molecular Identification of Forensically Important Indian Species of Flesh Flies (Diptera: Sarcophagidae) by Using COI Gene of Mitochondrial DNA

Sharma, Manish; Singh, Devinder

doi:10.4172/2157-7145.1000316

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…We found interspecific genetic distance range of flies was 5-20% (Table 3). Sharma et al (2015) studied ten Indian flesh fly species and showed the interspecific genetic divergence of 4-14%. Guo et al (2011) reported interspecific variation varying from 7% to 10% in Sarcophagid species.…”

Section: Discussionmentioning

confidence: 99%

“…DNA barcoding is gaining broad application in Integrated pest management (IPM) program as the standard method for species identification by matching unknown gene against the known (Etzler et al, 2014). A short standard region of the genome, specifically the mitochondrial gene, mitochondrial cytochrome c oxidase subunit I gene (MT-COI), is used in most of the cases (Hebert et al, 2003;Sharma et al, 2015) There is scarcity of information on medically and veterinary important flies especially through DNA barcoding in Bangladesh.…”

Section: Introductionmentioning

confidence: 99%

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Molecular identification and characterization of medically and veterinary important flies of Bangladesh based on mitochondrial COI gene sequences

Rain

Howlader

Aslam

2019

APJMBB

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Flies are considered serious pests which cause health problems of human and animal, transmitting many pathogenic microbes. Pest management programs depend on proper identification of pests. The present research work is an initiative to identify the medically and veterinary important flies based on mitochondrial COI gene sequences. Eleven species of the fly pests were identified. Among them, four fly species were the first record from Bangladesh. The phylogenetic analysis of retrieved sequences confirmed that the evolution of these species occurred from a common ancestor. Highest AT percentage (69.9%) was found in Haematobia irritans exigua and lowest GC percentage (30.4%) was found in Haematobia irritans exigua. The substitution rate of codon was found 1.88 in 1st position, 0.73 in 2nd position and 1.22 in 3rd position, respectively. Interspecific genetic divergence range of flies sequences was 5-20%. Haplotype network showed that Atylotus agrestis was mostly diverged from its common ancestors by 37 mutational steps. This research is the first molecular approach to identify the medically and veterinary important flies based on MT-COI gene sequences along with the establishment of first DNA barcode dataset for accurate identification in Bangladesh.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular identification and characterization of medically and veterinary important flies of Bangladesh based on mitochondrial COI gene sequences

Rain

Howlader

Aslam

2019

APJMBB

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“…Adults can be found on or near carcasses, which serve as a food source, breeding site, and substrate for immature development. This is why flesh flies are considered to be one of the most important indicators in forensic studies (Guo et al 2014, Sharma et al 2015, Fakoorziba et al 2017, Pavaraj et al 2018, Jang et al 2019, Oliveira et al 2019, Vairo & Moura 2021. Furthermore, Sarcophaginae are an ideal taxonomic group for ecological studies because they can be easily collected in a variety of environments using traps containing decomposing organic matter (Sousa et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Scavengers flesh flies (Diptera, Sarcophagidae) from two phytophysiognomies in the state of Maranhão, Northeastern of Brazil

2021

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Flesh flies (Sarcophagidae) have been found in a wide range of natural and anthropogenic environments, from forests to deserts. The state of Maranhão, located in Northeastern Brazil, has a diverse phytogeography, but few faunistic studies have been conducted in this area. Therefore, the objective of this study was to inventory species of Sarcophagidae and compare abundance patterns between the Cerrado (savanna-like vegetation) and riparian forests in the municipality of Codó, state of Maranhão. Twelve sampling events were carried out from the second half of 2015 to the first half of 2017. This resulted in 3,220 specimens, 27.15% of which were males, from nine genera and 27 species. A total of 491 specimens (16 species) were collected in the Cerrado, and 383 specimens (21 species) in the riparian forest. Peckia (Sarcodexia) lambens (Wiedemann) was the most abundant species (56,18% of the specimens sampled), followed by Peckia (Peckia) pexata (Wulp) (13%); Peckia (Euboettcheria) collusor (Curran & Walley) (13%), and Peckia (Peckia) chrysostoma Wiedemann (10%). Only P. (E.) collusor was found in greater abundance in the Cerrado. Oxysarcodexia angrensis (Lopes), Peckia (Peckia) enderleini (Engel), and Retrocitomiya andina Lopes are all new records to Maranhão and the entire Northeast Region of Brazil. Titanogrypa (Cuculomyia) albuquerquei (Lopes) is also a new to Maranhão.

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Molecular identification of forensically important fly species in Spain using COI barcodes

et al. 2020

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Molecular Identification of Forensically Important Indian Species of Flesh Flies (Diptera: Sarcophagidae) by Using COI Gene of Mitochondrial DNA

Cited by 4 publications

References 31 publications

Molecular identification and characterization of medically and veterinary important flies of Bangladesh based on mitochondrial COI gene sequences

Molecular identification and characterization of medically and veterinary important flies of Bangladesh based on mitochondrial COI gene sequences

Scavengers flesh flies (Diptera, Sarcophagidae) from two phytophysiognomies in the state of Maranhão, Northeastern of Brazil

Molecular identification of forensically important fly species in Spain using COI barcodes

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