The use of microcomputed tomography to study microvasculature in small rodents

Bentley, Michael D.; Ortiz, Mariela; Ritman, Erik L.; Romero, J. Carlos

doi:10.1152/ajpregu.00560.2001

Cited by 135 publications

(112 citation statements)

References 81 publications

(104 reference statements)

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“…The micro-computed tomography protocol is listed last (V), but should be considered to have comparable preparation complexity to protocol III. This protocol is optimized for ex vivo visualization of brain vasculature [Bentley et al, 2002].…”

Section: Imaging Protocolsmentioning

confidence: 99%

Mouse behavioral mutants have neuroimaging abnormalities

Nieman

Lerch

Bock

et al. 2007

Human Brain Mapping

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Impaired cognitive, memory, or motor performance is a distinguishing characteristic of neurological diseases. Although these symptoms are frequently the most evident in human patients, additional markers of disease are critical for proper diagnosis and staging. Noninvasive neuroimaging methods have become essential in this capacity and provide means of evaluating disease and tracking progression. These imaging methods are also becoming available to scientists in the research laboratory for assessment of animal models of neurological disease. Imaging in mouse models of neurological disease is of particular interest, owing to the availability of inbred strains and genetic manipulation tools that permit detailed investigation of the roles of various genes and gene products in disease pathogenesis. However, the relative prevalence of neuroimaging abnormalities in mice exhibiting neurological symptoms has not been reported. This prevalence has both theoretical and practical value because it is influenced by both the sensitivity of macroscopic anatomical measures to underlying genetic and disease processes and by the efficiency of neuroimaging in detecting and characterizing these effects. In this paper, we describe a meta-analysis of studies involving behavioral mouse mutants at our laboratory. In summary, we have evaluated 15 different mutant genotypes, of which 13 showed abnormal neuroimaging findings. This indicates a surprisingly high prevalence of neuroimaging abnormalities (87%) and suggests that disease processes affecting behavior generally alter neuroanatomy as well. As a consequence, neuroimaging provides a highly sensitive marker of neurological disease in mice exhibiting abnormal behavior. Hum Brain Mapp 28: [567][568][569][570][571][572][573][574][575] 2007. V V C 2007 Wiley-Liss, Inc.

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Section: Imaging Protocolsmentioning

confidence: 99%

Mouse behavioral mutants have neuroimaging abnormalities

Nieman

Lerch

Bock

et al. 2007

Human Brain Mapping

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“…A promising new technique, microcomputed tomography ( -CT) (Jorgensen et al, 1998), enables the study of the spatial arrangement of the vasa vasorum in large specimens (Lerman and Ritman, 1999;Bentley et al, 2002;Wan et al, 2002;Gössl et al, 2003), even in its frozen state (Kantor et al, 2002). In conjunction with semiautomated tree analysis software, it can provide quantitative geometrical data not yet available by other techniques (Gössl et al, 2003).…”

mentioning

confidence: 99%

Three‐dimensional arrangement of the vasa vasorum in explanted segments of the aged human great saphenous vein: Scanning electron microscopy and three‐dimensional morphometry of vascular corrosion casts

et al. 2004

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The vasa vasorum of skeletonized and nonskeletonized segments of five human great saphenous veins (GSVs), harvested during coronary bypass grafting, were cannulated, rinsed, and injected (casted) with the polymerizing resin Mercox-Cl-2B. After removal of the dry vascular tissue, the casts were examined using scanning electron microscopy. Stereopaired images (tilt angle, 6°) were taken, imported into a 3D morphometry system, and the 3D architecture of the vasa vasorum (arterial and venous vasa as well as capillaries) was studied qualitatively and quantitatively in terms of vasa diameters, intervascular and interbranching distances, and branching angles. Diameters of parent (d 0 ) and large (d 1 ) and small (d 2 ) daughter vessels of arterial and venous bifurcations served to calculate asymmetry ratios (␣) and area ratios (␤). Additionally, deviations of bifurcations and branching angles from optimal branches were calculated for selected arterial vasa. The arrangement of the vasa vasorum closely followed the longitudinally oriented connective tissue fibers in the adventitia and the circularly arranged smooth muscle cell layers within the outer layers of the media. Venous vasa by far outnumbered arterial vasa. Vasa vasorum changed their course several times in acute angles and revealed numerous circular constrictions, kinks, and outpouchings. Due to their spatial arrangement, the vasa vasorum are prone to tolerate vessel wall distension generated by acute increases in blood pressure or stretching of the vessel without severe impact on vessel functions. Preliminary comparisons of data from the bifurcations of cast arterial vasa vasorum, with calculated optimal bifurcations, do not yet give clear insights into the optimality principle(s) governing the design of arterial vasa vasorum bifurcations of the human GSVs.

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“…Similarly, Schneider et al [81] studied the murine hind limb vasculature using corrosion casts with both mCT and SR CT. Razavi et al [82] imaged the pulmonary and cardiac circulation using mCT. A review of mCT imaging of vascular networks can be found in Bentley et al [83] and more recently in Zagorchev et al [84].…”

Section: Micro-computed Tomographymentioning

confidence: 99%

Image-based modelling of skeletal muscle oxygenation

Zeller‐Plumhoff

Roose

Clough

et al. 2017

J. R. Soc. Interface.

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The supply of oxygen in sufficient quantity is vital for the correct functioning of all organs in the human body, in particular for skeletal muscle during exercise. Disease is often associated with both an inhibition of the microvascular supply capability and is thought to relate to changes in the structure of blood vessel networks. Different methods exist to investigate the influence of the microvascular structure on tissue oxygenation, varying over a range of application areas, i.e. biological in vivo and in vitro experiments, imaging and mathematical modelling. Ideally, all of these methods should be combined within the same framework in order to fully understand the processes involved. This review discusses the mathematical models of skeletal muscle oxygenation currently available that are based upon images taken of the muscle microvasculature in vivo and ex vivo. Imaging systems suitable for capturing the blood vessel networks are discussed and respective contrasting methods presented. The review further informs the association between anatomical characteristics in health and disease. With this review we give the reader a tool to understand and establish the workflow of developing an image-based model of skeletal muscle oxygenation. Finally, we give an outlook for improvements needed for measurements and imaging techniques to adequately investigate the microvascular capability for oxygen exchange.

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The use of microcomputed tomography to study microvasculature in small rodents

Cited by 135 publications

References 81 publications

Mouse behavioral mutants have neuroimaging abnormalities

Mouse behavioral mutants have neuroimaging abnormalities

Three‐dimensional arrangement of the vasa vasorum in explanted segments of the aged human great saphenous vein: Scanning electron microscopy and three‐dimensional morphometry of vascular corrosion casts

Image-based modelling of skeletal muscle oxygenation

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