The Use of Lacto-Propionic Orcein in Rapid Squash Methods for Chromosome Preparations

Dyer, A. F.

doi:10.3109/10520296309061169

Cited by 162 publications

(60 citation statements)

References 3 publications

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“…Chromosome studies were made from PMCs at meiosis. Flower buds were fixed in 3:1 Carnoy's fluid, and stained in lacto propionic orcein (Dyer 1963). Pollen fertility was assessed on the basis of stainability of pollen grains in 1:1 mixture of acetocarmine and glycerine.…”

mentioning

confidence: 99%

Cytology of the genus Cleome Linn.

Koshy

Mathew

1985

CYTOLOGIA

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mentioning

confidence: 99%

Cytology of the genus Cleome Linn.

Koshy

Mathew

1985

CYTOLOGIA

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“…The material was pretreated with a saturated solution of p-dichlorobenzene, fixed in a mixture of ethanol and acetic acid (3:1 v/v) and stored in 70% ethanol. The squash method and staining by lacto-propionic orceine were used (DYER, 1963).…”

Section: Plantsmentioning

confidence: 99%

Hieracium × grofae — a rediscovered diploid hybrid from the Ukrainian Carpathians

2006

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Diploid hybrid plants (2n = 18) between sexual diploid cytotypes of Hieracium alpinum and H. umbellatum were found in the Ukrainian Eastern Carpathians. They were identified with H. ×grofae WO L., originally used for the combination H. decipiens ×H. umbellatum var. lactaris. As H. decipiens sensu WO L. (non TAUSCH) does not produce viable pollen grains and is most probably a polyploid apomict, it is unlikely to produce diploid hybrid plants with diploid H. umbellatum. Both parent species, Hieracium alpinum and H. umbellatum are also given by WO LOSZCZAK from the original locality. Thus we conclude that H. ×grofae is result of hybridization between H. alpinum and high mountain form of H. umbellatum. Hybrid plants are morphologically intermediate between the parent species, and moreover resemble closely or they are identical with the experimental hybrids of the same parent combination. Hybrids produce rather high amount of homogeneously sized pollen grains (values of standard deviation and coefficient of variation lower then upper limits for diploids -3µm or 7.5%, respectively), and might probably serve as male parents in further crosses; on the other hand, they are fully seed-sterile. A lectotype of H. ×grofae, a second proved nothotaxon in the genus Hieracium s.str., is designated. Localities of H. ×grofae are located in subalpine belt of the Marmarosh Mts, the Svydovets' Mts and the Horhany Mts (all in the Ukrainian Eastern Carpathians). Biotopes of hybrid plants usually represent secondary formed and disturbed pastures allowing close contact of altitudinally vicariant parent taxa.

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“…For chromosome counts, root tips were collected in 8-hydroxyquinolin (350 mg 1-') and after 4 h at 17--20°C the tips were transferred to a 1:3 acetic acid/ethanol mixture, in which they were stored. Roots were then macerated in 1 N HC1 for 5 min at 60°C and the chromosomes were stained and squashed in lactopropionic orcein [ 15].…”

Section: Cytologymentioning

confidence: 99%

Breeding of a tomato genotype readily accessible to genetic manipulation

et al. 1986

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A tomato genotype, superior in regenerating plants from cell cultures, was obtained by transferring regeneration capacity from Lycopersicon peruvianum into L. esculentum by classical breeding. This genotype, MsK93, greatly facilitates genetic manipulation of tomato, as was demonstrated by successful leaf disc transformation using Agrobacterium tumefaciens and by direct gene transer to protoplasts derived from callus.

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The Use of Lacto-Propionic Orcein in Rapid Squash Methods for Chromosome Preparations

Cited by 162 publications

References 3 publications

Cytology of the genus Cleome Linn.

Cytology of the genus Cleome Linn.

Hieracium × grofae — a rediscovered diploid hybrid from the Ukrainian Carpathians

Breeding of a tomato genotype readily accessible to genetic manipulation

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