The Use of Lactic Acid Bacteria as Ruminant Probiotic Candidates Based on In Vitro Rumen Fermentation Characteristics

Abstract

This research was conducted to select, to identify LAB isolates and to investigate the effects of the LAB as probiotics candidate in the rumen fermentation. Nine isolates exhibited the potency as candidate probiotics for cattle. The experiment was arranged in randomized block design with ten treatments and three different times of in vitro as a block. The substrate consisted of 70% forage and 30% concentrate proportion. The substrate was incubated at 39oC using serum bottle of 100 ml capacity for fermentation. Approximately 0.75 g of substrates was put inside the serum bottle glass and filled with 73 ml of buffered rumen fluid and 2 ml of LAB inoculant. Gas production was measured every 2, 4, 6, 8, 10, 12, 24, 48 and 72 h of the incubation period. Gas production kinetic was estimated by the Ørskov’s equation. The LAB with the highest gas production, as probiotics candidate, were identified using partial 16S rDNA sequence. The results of this research indicated that nine LAB produced high gas production in the range of 193-198 ml compare to that of control (173 ml). The addition of LAB in rumen fermentation resulted in digestibility 65-75%, organic matter digestibility 51-73%, and 6.67-6.68 pH. Based on the molecular identification, 8 isolates are Lactobacillus plantarum and 1 of uncultured bacteria. The LAB strain 32 L. plantarum showed the best for a ruminant probiotic candidate based on the in vitro rumen fermentation characteristic.

Lactobacillus plantarum as feed additive to improvement in vitro ruminal biofermentation and digestibility of some tropical tree leaves

“…Our results agree with Ridwan et al . (2018) who reported that the amount of GP, values of b and the values of c have increased as a result of supplementing the diet with 8 LP strains compared with the nonadditive treatment. Furthermore, Elghandour et al .…”

In-vitro Evaluation of Three Conservation Methods of Probiotics from 42-day-old Tropical Calves

“…All the concentrations of probiotics had higher percentage of digestibility than the control (P = .05). The digestibility of A1D42 at 4x10 13 UFC/g concentrations had the greatest digestibility (66%), but it was no different with the A1D42 4x10 12 (Fig. 2).…”

“…Each digestion vessel received ten bags, 1600 mL (5:1) of the buffer solution, 400 mL of ruminal fluid and the probiotic at doses 4X10 11 , 4X10 12 and 4X10 13 UFC/g were added; the vessels were injected with CO 2 , and were positioned in the DAISY II digestor at 39 ºC for 48 h rotating. After that, the bags were washed, dried and were weighted to calculate the digestibility.…”

“…High feed digestibility and VFA production will provide more energy and allow it to utilize it as a source of fuel for body and animal production. When lactic bacteria (LAB) is added to rumen fluid, it has been demonstrated to boost digestibility [12][13][14].…”

Addition of cellulolytic bacteria in complete feed block based on agro-industrial by-products for Kacang goats

“…Organisms such as Lactobacillus, Streptococcus and Enterococcus are among the commonly used probiotics for ruminants (Seo et al, 2010). Ridwan et al (2018) reported that L. plantarum strain 32 isolated from cow rumen had higher in vitro dry matter and organic matter digestibilities and overall production of gas in comparison with the control. Oyeleke and Okusanmi (2008) stated that P. aeruginosa inhabited the rumen of cows, sheep, and goats and could hydrolyse cellulose.…”