The use of isolated functional heart to pharmacologically characterize active ingredients in the aqueous extracts of Mareya micrantha

Guédé-Guina, F.; Tsai, Chien‐Sung; Smith, Margaret O.; Vangah-Manda, M; Washington, Benny; Ochillo, Richard F.

doi:10.1016/0378-8741(94)01190-b

Cited by 9 publications

(7 citation statements)

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“…La présence de substances cholinomimétiques dans l'extrait aqueux de Bridelia ferruginea comme dans les extraits de Chrysanthemum trifurcatum [33] et de Mareya micrantha [51][52][53][54] pourrait justifier l'utilisation traditionnelle de ces plantes contre les problèmes grastro-intestinaux, en particulier la constipation [55,33].…”

Section: Discussionunclassified

Etudes phytochimique et pharmacologique de Bridelia ferruginea benth (euphorbiaceae) sur la motricite du Taenia coli de cobaye

Bi¹,

Traoré²,

Soro³

et al. 2010

Afrique Science: Revue Internationale Des Sciences Et Technologie

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RésuméL'étude phytochimique des extraits de Bridelia ferruginea a montré que cette plante contient des quinones, des tanins catéchiques et galliques, des alcaloïdes, des stérols, des polyterpènes, des polyphénols, des composés réducteurs, des flavonoïdes et des saponosides.L'extrait aqueux de Bridelia ferruginea provoque entre 10 -5 mg/mL et 10 -1 mg/mL une augmentation des contractions rythmiques de muscle lisse Taenia coli de cobaye. Ces effets inotropes positifs antagonisés par l'Atropine (10 -6 mg/mL) sont totalement abolis en milieu pauvre (0Ca 2+ ) et dépourvus de calcium (0Ca 2+ + EDTA 10 -5 M).Ces résultats suggèrent la présence de substances cholinomimétiques dans l'extrait brut. Ces substances favoriseraient l'entrée de calcium dans la cellule musculaire lisse par stimulation des récepteurs muscariniques. Ces substances cholinomimétiques justifieraient l'usage traditionnel de cette plante comme laxatif ou purgatif. Mots-clés :Bridelia ferruginea, Taenia coli, récepteur muscarinique. AbstractPhytochemical and pharmacological studies of bridelia ferruginea benth (euphorbiaceae) on guinea pig taenia coli motility.The phytochemical study of Bridelia ferruginea extracts revealed that the plant contained quinones, catechic and gallic tannins, alkaloids, sterols, polyterpenes, polyphenols, reducing compounds, flavonoids and saponins. The aqueous extract of Bridelia Afrique SCIENCE 05 (2) (2009) 305 -320 Semi Anthelme NENE BI et al. 306ferruginea, for concentrations ranging from 10 -5 mg/mL to 10 -1 mg/mL, caused an increase of the rhythmical contraction of guinea pig Taenia coli smooth muscle. These positive inotropic effects inhibited by Atropine (10 -6 mg/mL) were completely abolished in poor calcium mediums (0Ca 2+ ; 0Ca 2+ + EDTA 10 -5 M). The results suggested the presence of cholinomimetic substances in the crude extract of Bridelia ferruginea. Therefore, the inward calcium flow into the smooth muscle cell would be due to the stimulation of muscarinic cholinoceptors induced by these substances. In conclusion, the traditional use of this plant as a laxative is justified by the presence of these cholinomimetic substances.

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Section: Discussionunclassified

Etudes phytochimique et pharmacologique de Bridelia ferruginea benth (euphorbiaceae) sur la motricite du Taenia coli de cobaye

Bi¹,

Traoré²,

Soro³

et al. 2010

Afrique Science: Revue Internationale Des Sciences Et Technologie

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“…Previous studies showed that the aqueous leaf extracts of M. micrantha suppressed cardiac contractility of isolated frog and rat hearts in a concentration dependent way [8,9]. In another test, an aqueous leaf extract elicited concentration-dependent contractions of the longitudinal muscle of isolated guinea-pig ileum [10].…”

Section: Introductionmentioning

confidence: 99%

Laxative activities of Mareya micrantha (Benth.) Müll. Arg. (Euphorbiaceae) leaf aqueous extract in rats

Souleymane

Calixte

Yeo

et al. 2010

BMC Complement Altern Med

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BackgroundMareya micrantha (Benth.) Müll. Arg. (Euphorbiaceae) is a shrub that is commonly used in Côte d'Ivoire (West Africa) for the treatment of constipation and as an ocytocic drug. The present study was carried out to investigate the laxative activity of Mareya micrantha in albino's Wistar rats.MethodsRats were divided in 5 groups of 5 animals each, first group as control, second group served as standard (sodium picosulfate) while group 3, 4 and 5 were treated with leaf aqueous extract of Mareya micrantha at doses of 100, 200 and 400 mg/kg body weight (b.w.), per os respectively. The laxative activity was determined based on the weight of the faeces matter. The effects of the leaves aqueous extract of Mareya micrantha and castor oil were also evaluated on intestinal transit, intestinal fluid accumulation and ions secretion.ResultsPhytochemicals screening of the extract revealed the presence of flavonoids, alkaloids, tannins, polyphenols, sterols and polyterpenes. The aqueous extract of Mareya micrantha applied orally (100, 200 and 400 mg/kg; p.o.), produced significant laxative activity and reduced loperamide induced constipation in dose dependant manner. The effect of the extract at 200 and 400 mg/kg (p.o.) was similar to that of reference drug sodium picosulfate (5 mg/kg, p.o). The same doses of the extract (200 and 400 mg/kg, p.o.) produced a significant increase (p < 0.01) of intestinal transit in comparison with castor oil (2 mL) (p < 0.01). Moreover, the extract induced a significant enteropooling and excretion of Cl-, Na+, K+ and Ca2+ in the intestinal fluid (p < 0.01).ConclusionsThe results showed that the aqueous extract of Mareya micrantha has a significant laxative activity and supports its traditional use in herbal medicine.

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“…Cet intérêt particulier accordé aux plantes médicinales nous a permis, à partir de certaines d'entre elles, d'isoler plusieurs substances d'origine naturelle à la base de médicaments conventionnels. C'est le cas de l'atropine, extraite de Atropa belladona, et de Mansonia altissima (sterculiacées) dont la mansonine (MSN) demeure le principal composé purifié pouvant être utilisé dans le traitement des affections cardiovasculaires [10][11][12][13][14].…”

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Effet de Mitragyna ciliata (MYTA) sur le système cardiovasculaire de rat

Bidié¹,

Yapo²,

Yeo³

et al. 2010

Phytothérapie

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Dans cette étude, nous avons évalué, d'une part, l'influence d'un extrait méthanolique de Mitragyna ciliata (MYTA) [rubiacées] sur l'activité mécanique du coeur isolé de rat, grâce au dispositif amélioré de Langerdorff et, d'autre part, la pression artérielle carotidienne de lapin grâce au dispositif de Ludwig. MYTA induit un effet inotrope positif qui varie de 0 à 35 ± 0,89 % d'augmentation pour des concentrations allant de 10 -5 mg/ml à 5 Â 10 -1 mg/ml et à une concentration très élevée de 1 mg/ml, le coeur s'arrête avec une amplitude de plus de 50 ± 1,29 % d'augmentation par rapport au témoin. Il induit aussi un effet chronotrope négatif dose-dépendant qui diminue jusqu'à 48 ± 0,79 %, avant de s'annuler avec la concentration de 1 mg/ml. De plus, MYTA régularise progressivement l'activité cardiaque pour des arythmies induites par l'atropine et l'hypothermie. Ainsi, MYTA renforce, régularise et ralentit l'activité cardiaque. MYTA induit une chute de tension dose-dépendante qui varie de 140 mmHg (valeur normale) à 129 mmHg pour une dose allant de 10 à 40 mg/kg de poids corporel (PC). Mots clés : MYTA -Cardiovasculaire -CardiotoniqueHypotensifEffect of Mitragyna ciliata (MYTA) on cardiovascular system of rat Abstract : In this study, we evaluate the influence of a methanolic extract of Mitragyna ciliata (MYTA) [Rubiaceae] on the mechanics of isolated rat heart, thanks to improved Langerdorff's device and carotid arterial pressure of rabbits with Ludwig's device. MYTA, in concentrations ranging from 10 -5 mg/ml to 5 Â 10 mg/ml induces a drop in blood pressure with inotropic effect that varies from 0 to 35 ± 0.89% increase in concentrations ranging from 10 -5 mg/ml at 5 Â 10 -1 mg/ml and a very high concentration of 1 mg/ml stops the heart with a magnitude of more than 50 ± 1.29% increase compared with the control. It also induces a dosedependent negative chronotropic effect decreasing to 48 ± 0.79% before vanishing with the concentration of 1 mg/ ml. In addition, MYTA gradually regulates the activity of cardiac arrhythmia induced by atropine and hypothermia. Thus, MYTA strengthens, regulates, and slows the activity of heart. MYTA induces dose-dependent hypotension ranging from 140 mmHg (normal) to 129 mmHg for a dose of 40-mg/kg body weight.

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The use of isolated functional heart to pharmacologically characterize active ingredients in the aqueous extracts of Mareya micrantha

Cited by 9 publications

References 3 publications

Etudes phytochimique et pharmacologique de <i>Bridelia ferruginea</i> benth (euphorbiaceae) sur la motricite du <i>Taenia coli</i> de cobaye

Etudes phytochimique et pharmacologique de <i>Bridelia ferruginea</i> benth (euphorbiaceae) sur la motricite du <i>Taenia coli</i> de cobaye

Laxative activities of Mareya micrantha (Benth.) Müll. Arg. (Euphorbiaceae) leaf aqueous extract in rats

Effet de Mitragyna ciliata (MYTA) sur le système cardiovasculaire de rat

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