The use of iodinated steroid as radioligand for testosterone radioimmunoassay

Hampl, Richard; Dvořák, Pavel; Lukesová, S; Kozák, I.; Chrpová, M; Stárka, L.

doi:10.1016/0022-4731(78)90198-x

Cited by 27 publications

(4 citation statements)

References 6 publications

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“…Testosterone levels were determined as described elsewhere [10]. Radioimmunoassay was carried out after diethylether extraction using rabbit polyclonal antiserum against testosterone-3-CMO:BSA and radioiodine labeled testosteronetyrosin methylester as a tracer.…”

Section: Laboratory Methodsmentioning

confidence: 99%

Free testosterone and free dihydrotestosterone throughout the life span of men

Stárka

Pospíšilová

Hill

2009

The Journal of Steroid Biochemistry and Molecular Biology

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Section: Laboratory Methodsmentioning

confidence: 99%

Free testosterone and free dihydrotestosterone throughout the life span of men

Stárka

Pospíšilová

Hill

2009

The Journal of Steroid Biochemistry and Molecular Biology

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“…The tissue steroids were extracted and cleaned according to Scheme 1 by liquid chromatography under the conditions given in detail elsewhere (13), the plasmatic steroids were determined without Chromatographie purification, the plasma epitestosterone was determined by the extraction technique according to Bilek (5). The epitestosterone was determined by the original radioimmunological method (5), testosterone, androstenedione and dihydrotestosterone were determined by conventional radioimmunoassay (14)(15)(16). For the method of steroid determination in prostatic tissue the recovery was checked by spiking with 500 pg of each steroid per g of tissue.…”

Section: Determination Of Steroidsmentioning

confidence: 99%

Epitestosterone in Human Blood and Prostatic Tissue

Stárka

Hampl²,

Hill³

et al. 1997

Clinical Chemistry and Laboratory Medicine

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Epitestosterone, a Ci 9-steroid with anti-androgenic activity, was determined in the plasma of 234 boys and men from the ages of 6-86 years, and in the prostate tissue of 15 men 55-82 years of age. It was documented that, while in adulthood the concentration of epitestosterone is about ten times lower than the concentration of testosterone, in the pre-pubertal period the level of epitestosterone is similar or even higher than that of testosterone. In the hyperplastic prostate tissue the content of epitestosterone is comparable to that of androstenedione, it is about twice as high as the content of testosterone and approximately half that of the content of dihydrotestosterone. At least in the case of pre-pubertal boys and in the prostatic tissue it is therefore possible to include epitestosterone into consideration as a regulatory factor for the androgen-dependent events.

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“…The anthropometrie changes were evaluated always after termination of treatment. Testosterone in plasma, urine and saliva was estimated by radioimmunoassay (Hampl, 1978), and so were plasma oestradiol, LII, FSH and cortisol (Hampi et al, 1985). The TeBG binding capacity was assessed by gel dialysis on Sephadex (Pearlman, 1979).…”

Section: Methodsmentioning

confidence: 99%