The use of hyaluronan to regulate protein adsorption and cell infiltration in nanofibrous scaffolds

Li, Linhao; Qian, Yuna; Jiang, Chao; Lv, Yonggang; Liu, Wanqian; Zhong, Li; Cai, Kaiyong; Li, Song; Yang, Li

doi:10.1016/j.biomaterials.2012.01.038

Cited by 115 publications

(64 citation statements)

References 58 publications

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“…It is an ECM component of many soft tissues in higher animals, in which it maintains hydration and induces cell proliferation, migration, and angiogenesis. HA nanofibers have already been tested in the development of modern wound dressings (Beachley and Wen, 2010;Li et al, 2012;Uppal et al, 2011). The main drawbacks of HA nanofibers are related to poor cell adhesion, mechanical instability, and rapid degradation in vivo.…”

Section: Polymers Used For Nanofiber Productionmentioning

confidence: 99%

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Critical attributes of nanofibers: Preparation, drug loading, and tissue regeneration

Pelipenko

Kocbek

Kristl

2015

International Journal of Pharmaceutics

197

149

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Section: Polymers Used For Nanofiber Productionmentioning

confidence: 99%

“…When a polymer with desirable characteristics is selected, the next step is to prepare the polymer dispersion (Bhardwaj and Kundu, 2010;Li et al, 2012). The first requirement of the solvent is to dissolve the polymer in sufficient concentration for nanofiber formation.…”

Section: Solvents Used For Nanofiber Productionmentioning

confidence: 99%

Critical attributes of nanofibers: Preparation, drug loading, and tissue regeneration

Pelipenko

Kocbek

Kristl

2015

International Journal of Pharmaceutics

197

149

View full text Add to dashboard Cite

“…To this end, many groups have employed surface modification strategies to alter the surface properties of EFs to reduce protein adsorption. One strategy that has been most successful in reducing protein adsorption is conjugating polyethylene glycol (PEG) to the surface of polymeric vehicles in brush-like or other configurations (99)(100)(101). Similarly, copolymers, such as monomethoxy-PEG-PLGA, may be used to fabricate fibers that contain similar reactive groups while eliminating nonspecific adsorption to the fiber surface.…”

Section: Discussionmentioning

confidence: 99%

Griffithsin-Modified Electrospun Fibers as a Delivery Scaffold To Prevent HIV Infection

Grooms

Vuong

Tyo

et al. 2016

Antimicrob Agents Chemother

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Despite current prophylactic strategies, sexually transmitted infections (STIs) remain significant contributors to global health challenges, spurring the development of new multipurpose delivery technologies to protect individuals from and treat virus infections. However, there are few methods currently available to prevent and no method to date that cures human immunodeficiency virus (HIV) infection or combinations of STIs. While current oral and topical preexposure prophylaxes have protected against HIV infection, they have primarily relied on antiretrovirals (ARVs) to inhibit infection. Yet continued challenges with ARVs include user adherence to daily treatment regimens and the potential toxicity and antiviral resistance associated with chronic use. The integration of new biological agents may avert some of these adverse effects while also providing new mechanisms to prevent infection. Of the biologic-based antivirals, griffithsin (GRFT) has demonstrated potent inhibition of HIV-1 (and a multitude of other viruses) by adhering to and inactivating HIV-1 immediately upon contact. In parallel with the development of GRFT, electrospun fibers (EFs) have emerged as a promising platform for the delivery of agents active against HIV infection. In the study described here, our goal was to extend the mechanistic diversity of active agents and electrospun fibers by incorporating the biologic GRFT on the EF surface rather than within the EFs to inactivate HIV prior to cellular entry. We fabricated and characterized GRFT-modified EFs (GRFT-EFs) with different surface modification densities of GRFT and demonstrated their safety and efficacy against HIV-1 infection in vitro. We believe that EFs are a unique platform that may be enhanced by incorporation of additional antiviral agents to prevent STIs via multiple mechanisms. N ewly acquired sexually transmitted infections (STIs) affect 340 million people per year (1-3) and exert a significant impact on global health. Human immunodeficiency virus type 1 (HIV-1) affects ϳ35 million people globally (2-5), while untreated STIs, such as those caused by herpes simplex virus 2 (HSV-2), can enhance both the acquisition and the transmission of HIV and other agents of STIs by 2-to 4-fold (6, 7). In light of the findings of recent clinical trials, a specific, multipurpose prevention technology that has the ability to prevent multiple STIs using one delivery platform and that also increases user adherence urgently needs to be developed (8-18). In the study described here, we sought to shift current topical preexposure prophylaxis (PrEP) paradigms by integrating a multipurpose biological delivery approach to debilitate and inactivate HIV.Our long-term goal is to develop a multipurpose biologically inspired electrospun fiber (EF) prevention technology that takes cues from the innate microenvironment of the female reproductive tract to more strategically narrow the gaps in microbicide efficacy. In this work, we evaluated the potential of polymeric EF scaffolds surface modified with the po...

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“…The anti-BSA adsorption trend was PHBP-H4> PHBP-P4> PHB-PAMAM, which is similar to the hydrophilicity trend of the samples shown in Figure S3. As the hydrophobic surface was prone to protein adsorption 26 , the hydrophilic HA in the multilayers could inhibit BSA adsorption 27,28 . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 13 …”

Section: Bsa Adsorption Assaymentioning

confidence: 99%

Antimicrobial Hyaluronic Acid/Poly(amidoamine) Dendrimer Multilayer on Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Prepared by a Layer-by-Layer Self-Assembly Method

Zhan

Wang

Liu

et al. 2015

ACS Appl. Mater. Interfaces

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In this article, we prepared hyaluronic acid/poly(amidoamine) dendrimer (HA/PAMAM) multilayers on a poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-4HB)] substrate by a layer-by-layer self-assembly method for antimicrobial biomaterials. The results of ζ potential and quartz crystal microbalance with dissipation (QCM-D) showed that HA/PAMAM multilayers could be formed on the substrate layer by layer. We used QCM-D to show that both the HA outer layer and the PAMAM outer layer exhibited good protein-resistant activity to bovine serum albumin and bacterial antiadhesion activity to Escherichia coli. By a live/dead assay and the colony counting method, we found that the PAMAM outer layer could also exhibit bactericidal activity against E. coli, while the HA outer layer had no bactericidal activity. Both the bacterial antiadhesion activity and the bactericidal activity of the samples could be maintained even after storage in phosphate-buffered saline for up to 14 days. An in vitro MTT assay showed that the multilayers had no cytotoxicity to L929 cells, and HA molecules in the multilayers could improve the biocompatibility of the film.

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The use of hyaluronan to regulate protein adsorption and cell infiltration in nanofibrous scaffolds

Cited by 115 publications

References 58 publications

Critical attributes of nanofibers: Preparation, drug loading, and tissue regeneration

Critical attributes of nanofibers: Preparation, drug loading, and tissue regeneration

Griffithsin-Modified Electrospun Fibers as a Delivery Scaffold To Prevent HIV Infection

Antimicrobial Hyaluronic Acid/Poly(amidoamine) Dendrimer Multilayer on Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Prepared by a Layer-by-Layer Self-Assembly Method

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