The Use of Human Sweat Gland–Derived Stem Cells for Enhancing Vascularization during Dermal Regeneration

Danner, Sandra; Kremer, Mathias; Petschnik, Anna Emilia; Nagel, Sabine; Zhang, Ziyang; Hopfner, Úrsula; Reckhenrich, Ann K.; Weber, Caroline; Schenck, Thilo L.; Becker, Tim; Kruse, Charli; Machens, Hans‐Günther; Egaña, José Tomás

doi:10.1038/jid.2012.31

Cited by 47 publications

(33 citation statements)

References 33 publications

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“…After digital segmentation, vessels could be easily quantified at desired times post implantation. For instance, 2 weeks after implantation, vascularization levels of 62.28 ± 8.6% were observed (mean ± standard error of mean; n=8) 21 . Altogether, about 25 min per animal (2 scaffolds) are required for the implantation, 5 min for tissue harvesting and picturing and 10 min for digital analysis of one sample.…”

Section: Representative Resultsmentioning

confidence: 98%

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials <em>In Vivo</em>

Schenck

Chávez

Condurache

et al. 2014

JoVE

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Insufficient vascularization is considered to be one of the main factors limiting the clinical success of tissue-engineered constructs. In order to evaluate new strategies that aim at improving vascularization, reliable methods are required to make the in-growth of new blood vessels into bioartificial scaffolds visible and quantify the results. Over the past couple of years, our group has introduced a full skin defect model that enables the direct visualization of blood vessels by transillumination and provides the possibility of quantification through digital segmentation. In this model, one surgically creates full skin defects in the back of mice and replaces them with the material tested. Molecules or cells of interest can also be incorporated in such materials to study their potential effect. After an observation time of one's own choice, materials are explanted for evaluation. Bilateral wounds provide the possibility of making internal comparisons that minimize artifacts among individuals as well as of decreasing the number of animals needed for the study. In comparison to other approaches, our method offers a simple, reliable and cost effective analysis. We have implemented this model as a routine tool to perform high-resolution screening when testing vascularization of different biomaterials and bio-activation approaches. Video LinkThe video component of this article can be found at

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Section: Representative Resultsmentioning

confidence: 98%

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials <em>In Vivo</em>

Schenck

Chávez

Condurache

et al. 2014

JoVE

Self Cite

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“…A biopsy of human axillary skin is sufficient and avoids any surgical intervention [28]. In an in vivo full-thickness skin defect, hSGSCs were able to improve vascularisation during scaffold-dependent dermal regeneration [22]. The secretion of factors involved in vascularisation, immune regulation and tissue remodeling was found [21].…”

Section: Discussionmentioning

confidence: 99%

“…The isolation of stem cells from human sweat glands yields a multipotent and highly proliferative Nestin-positive cell population [21]. The regenerative potential of hSGSCs was already proven in dermal wound healing where it promoted vascularisation [22]. Furthermore, the feasibility of targeted neuronal differentiation in a xenogenic co-culture system describes the clinical relevance of hSGSCs [20].…”

Section: Introductionmentioning

confidence: 99%

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Co-Culture Systems of Human Sweat Gland Derived Stem Cells and Peripheral Nerve Cells: Anin VitroApproach for Peripheral Nerve Regeneration

Mehnert

Kisch

Brandenburger

2014

Cell Physiol Biochem

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Background/Aims: The treatment of peripheral nerve lesions still represents a clinical challenge. Several approaches such as novel biomaterials for nerve guides, addition of growth factors or cellular supplements moved in the focus of research. Especially the application of autologous stem cells is highly promising for future applications. Human sweat gland derived stem cells (hSGSCs) represent an easy accessible source of autologous adult stem cells and did already show a beneficial effect in dermal wound healing. Methods: In this study, the effect of hSGSCs on neurite outgrowth of primary adult or prenatal Dorsal root ganglia (DRG) neurons was analysed in an indirect co-culture model. Additionally, direct co-cultures with hSGSCs as a feeder layer were performed. Results: Adult and prenatal DRG neurons showed increased neurite outgrowth after 24 h co-culture with hSGSCs. The outgrowth increased significantly by the factors 5.6 and 2.6 respectively. Direct co-cultures revealed neurite alignment along the hSGSCs orientation. Conclusion: The paracrine influence of hSGSCs on neurite outgrowth, but also their ability to operate as a feeder layer with guidance properties shows great potential for future applications in peripheral nerve regeneration.

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“…The transplantation of keratinocytes is achieved thanks for obtaining large amounts of cultured cells from a skin biopsy in 3-4 weeks [12]. One of the benefits of using stem cells is their ability to migrate and differentiate the endothelium [13], which promotes revascularization [14]. The increased optimization of microvascular activity is the result of the injection of stem cells into a protracted wound [13].…”

Section: Cellular Therapiesmentioning

confidence: 99%

Advanced Technologies in Dermatology

Kitala¹,

Klama-Baryła²,

Łabuś³

et al. 2018

Dermatologic Surgery and Procedures

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Cellular therapies are an attractive area of regenerative medicine. For large partial thickness wound, keratinocytes transplant is suggested. The transplantation of cell graft is achieved by obtaining large amounts of cultured cells from a skin biopsy in 3 weeks. Stem cells can be applied before that, but are also efficient in chronic wound closure. Alternative treatment methods are transplants of allogeneic, biostatic skin and amnion. Amnion can be applied as a skin substitute on shallow facialburn wounds, hand burn wounds, on donor areas and granulating wounds. For medium depth or even deep burns, allogeneic skin is recommended. Thanks to the removing of cells from human allogeneic dermis, collagen scaffolding is obtained. It can be populated de novo by autologous skin cells. Artificial skin substitutes are especially good for hand burns and shallow burns. Even though scarring is a part of normal wound healing, it often leads to a pathological process. When scar treatment methods prove insufficient, surgical intervention becomes necessary. Surgical scar intervention involves removal of the pathological skin tissue fragment and replacing it with healthy skin or application of expanders. Improvement of the visual features can be also achieved by laser therapy.

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The Use of Human Sweat Gland–Derived Stem Cells for Enhancing Vascularization during Dermal Regeneration

Cited by 47 publications

References 33 publications

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials <em>In Vivo</em>

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials <em>In Vivo</em>

Co-Culture Systems of Human Sweat Gland Derived Stem Cells and Peripheral Nerve Cells: Anin VitroApproach for Peripheral Nerve Regeneration

Advanced Technologies in Dermatology

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