The Use of HRP in Decolorization of Reactive Dyes and Toxicological Evaluation of Their Products

Abstract

This work studied the potential use of horseradish peroxidase (HRP) in the decolorization of the following textile dyes: Drimarene Blue X-3LR (DMBLR), Drimarene Blue X-BLN (DMBBLN), Drimarene Rubinol X-3LR (DMR), and Drimarene Blue CL-R (RBBR). Dyes were individually tested in the reaction media containing 120 mg·L−1, considering the following parameters: temperature (20–45°C), H2O2 concentration (0–4.44 mmol·L−1), and reaction time (5 minutes, 1 and 24 h). The following conditions: 35°C, 0.55 mmol·L−1, and 1h, provided the best set of results of color removal for DMBLR (99%), DMBBLN (77%), DMR (94%), and RBBR (97%). It should be mentioned that only 5 minutes of reaction was enough to obtain 96% of decolorization for DMBLR and RBBR. After the decolorization reactions of DMBLR, DMR, and RBBR, it was possible to observe the reduction of Artemia salina mortality and the no significant increase in toxicity for the products generated from DMBBLN.

Phytoremediation: Potential flora for synthetic dyestuff metabolism

“…Natural dyes, extracted from plant or animal sources, are used in the coloring of food stuff, leather and natural protein fibers such as silk, cotton and wool. However, the provision of narrow or dull range of colors along with exhibition of low color fastness on exposure to washing and sunlight has limited their applicability (Maddhinni et al, 2006;Samanta and Agarwal, 2009;da-Silva et al, 2010). Conversely, aromatic compounds produced via chemical synthesis, termed as synthetic dyes, provide a wide range of colors that are colorfast and bright as well (Kant, 2012).…”

“…Studies elucidate that majority of industries preferably utilize synthetic dyes for coloring different materials in contrast to natural dyes due to higher efficiency, wide range of colors, bright color imparting properties, stability and resistance against fading as well as washing (You et al, 2010;da-Silva et al, 2010). According to McMullan et al (2001) and Selvam et al (2003) commercially available synthetic dyes comprise a fraction of greater than 100,000 of currently known dyes and approximately one million tons of these dyes have been estimated to be produced on annual basis at the international level.…”

The application of enzymes for the removal of dyes from textile effluents

“…Both flocculation and adsorption generate large amounts of toxic sludge and waste, which require a separate treatment before disposal [16]. However, this method always has high costs associated due to the reagents involved.…”

Enzymatic decolourisation of Methyl Orange and Bismarck Brown using crude peroxidase from Armoracia rusticana

“…Enzymatic degradation of pollutants in wastewater is fast gaining importance as an alternative or a supplement to conventional effluent treatment practices (Vijaykumar et al 2006;da Silva et al 2010). Enzymes are efficient biological catalysts and can operate under mild conditions of pH and temperature.…”