The use of high-hydrostatic pressure treatment to decellularize blood vessels

Funamoto, Seiichi; Nam, Kwangwoo; Kimura, Tsuyoshi; Murakoshi, Ayako; Hashimoto, Yoshihide; Niwaya, Kazuo; Kitamura, Soichiro; Fujisato, Toshiya; Kishida, Akio

doi:10.1016/j.biomaterials.2010.01.073

Cited by 240 publications

(199 citation statements)

References 24 publications

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“…On the other hand, several reports have demonstrated SDS to cause strong structural alterations (Kasimir et al, 2003). It was also noted that this ionic detergent tends to cause a decrease in glycosaminoglycan (GAG) concentration and a loss of collagen integrity (Funamoto et al, 2010;Crapo et al, 2011;Dong et al, 2013). This controversial finding seems to be a consequence of the different incubation times and SDS concentrations used in the decellularization procedure.…”

Section: Discussionmentioning

confidence: 99%

Preparation and characterization of acellular porcinepericardium for cardiovascular surgery

Tran¹,

Dinh²,

Nguyen³

et al. 2016

Turk J Biol

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The aim of this study was to fabricate and characterize acellular porcine pericardium that can be used to patch cardiovascular repair. Porcine pericardial tissues were treated with 10 mM Tris-HCl and sodium dodecyl sulfate (SDS) at different concentrations and time intervals. The optimal decellularization protocol was determined according to elimination of cellular components and DNA via histology and DNA quantification, respectively. There were no significant changes in stress and fracture strain after decellularization. Liquid extracts from the decellularized pericardium caused no cytotoxicity towards human fibroblasts, were capable of supporting an appropriate attachment of human endothelial progenitor cells, and did not cause a local inflammatory effect after 30 days of transplantation in mice.

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Section: Discussionmentioning

confidence: 99%

Preparation and characterization of acellular porcinepericardium for cardiovascular surgery

Tran¹,

Dinh²,

Nguyen³

et al. 2016

Turk J Biol

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“…However, these methods are ineffective for thicker tissues (>5 mm). 16 By contrast, enzymatic digestion disintegrates the cell membrane, and also damages the extracellular matrix of collagen and elastin, causing disruption of the decellularized matrix structure, 17,18 which is not beneficial for cell regeneration. Therefore, enzyme digestion was not considered for DSM preparation in this study.…”

Section: Discussionmentioning

confidence: 99%

Hepatocyte Culture in Autologous Decellularized Spleen Matrix

Gao¹,

Xiang³

et al. 2015

Organogenesis

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ABSTRACT. Background and Aims: Using decellularized scaffold to reengineer liver tissue is a promising alternative therapy for end-stage liver diseases. Though the decellularized human liver matrix is the ideal scaffold for reconstruction of the liver theoretically, the shortage of liver donors is still an obstacle for potential clinical application. Therefore, an appropriate alternative scaffold is needed. In the present study, we used a tissue engineering approach to prepare a rat decellularized spleen matrix (DSM) and evaluate the effectiveness of this DSM for primary rat hepatocytes culture. Methods: Rat decellularized spleen matrix (DSM) was prepared by perfusion of a series of detergents through spleen vasculature. DSM was characterized by residual DNA and specific extracellular matrix distribution. Thereafter, primary rat hepatocytes were cultured in the DSM in a 3-dimensional dynamic culture system, and liver cell survival and biological functions were evaluated by comparison with 3-dimensional sandwich culture and also with cultured in decellularized liver matrix (DLM). Results: Our research found that DSM did not exhibit any cellular components, but preserved the main extracellular matrix and the intact vasculature evaluated by DNA detection, histology, immunohistochemical staining, vessel corrosion cast and upright metallurgical microscope. *Correspondence to: Yi Lv; Email: luyi169@126.com Received July 7, 2014; Revised September 10, 2014; Accepted January 18, 2015.16 Organogenesis, 11:16-29, 2015 Ó 2015 Taylor & Francis Group, LLC ISSN: 1547-6278 print / 1555-8592 online DOI: 10.1080/15476278.2015 Moreover, the method of DSM preparation procedure was relatively simple with high success rate (100%). After seeding primary hepatocytes in DSM, the cultured hepatocytes survived inside DSM with albumin synthesis and urea secretion within 10 d. Additionally, hepatocytes in dynamic culture medium had better biological functions at day 10 than that in sandwich culture. Albumin synthesis was 85.67 § 6.34 mg/10 7 cell/24h in dynamic culture in DSM compared to 62.43 § 4.59 mg/10 7 cell/ 24h in sandwich culture (P < 0.01) and to 87.54 § 5.25 mg/10 7 cell/24h in DLM culture (P > 0.05); urea release was 32.14 § 8.62 mg/10 7 cell/24h in dynamic culture in DSM compared to 20.47 § 4.98 mg/10 7 cell/24h in sandwich culture (P < 0.05) and to 37.38 § 7.29 mg/10 7 cell/24h cultured in DLM (P > 0.05). Conclusion: The present study demonstrates that DSM can be prepared successfully using a tissue engineering approach. The DSM is an appropriate scaffold for primary hepatocytes culture.

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“…Rectangular or dumb-bell shaped specimens cut from the opened strips of tissue produce different mechanical responses in both the axial and circumferential directions (Amiel et al, 2006, Funamoto et al, 2010. However this damage is not sufficient to cause significant adverse effects on the tensile response of the tissue.…”

Section: Discussionmentioning

confidence: 99%