The use of global transcriptional analysis to reveal the biological and cellular events involved in distinct development phases of Trichophyton rubrum conidial germination

Liu, Tao; Zhang, Qian; Wang, Lingling; Yu, Lu; Leng, Wenchuan; Yang, Jian; Chen, Lihong; Peng, Junping; Ma, Li; Dong, Jie; Xu, Xingye; Xue, Ying; Zhu, Yafang; Zhang, Wenliang; Li, Yang; Li, Weijun; Sun, Lu; Wan, Zhe; Ding, Guohui; Yu, Fudong; Tu, Kang; Zhou, Qian; Li, Ruoyu; Shen, Yan; Li, Yixue; Jin, Qi

doi:10.1186/1471-2164-8-100

Cited by 59 publications

(57 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The prestored mRNA in conidia can be activated and translated rapidly in the presence of nutrients and soon after germination the stored mRNA decayed [21]. In a previous study, we found stored mRNA in T. rubrum conidia and this had similar expression characteristics to the mRNA of the Cluster I genes in this study [13].…”

Section: Discussionmentioning

confidence: 54%

“…A reference genomic DNA fragment (5 μg) isolated in the hyphal stage was labeled with Cy3 as previous report [13].…”

Section: Microarray Hybridizationmentioning

confidence: 99%

“…These sequences represent 85% of the predicated genes [11,12]. Using complementary DNA microarray technology, we analyzed changes in gene expression during conidia germination and reported the effects of several antifungal agents on the gene expression profile of T. rubrum [13][14][15]. In the present study, we investigated the growth dynamics and characterized the changes of gene expression that take place during the period when T. rubrum was cultured in Sabouraud liquid medium.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Global gene expression profiles for the growth phases of Trichophyton rubrum

Liu

Leng

et al. 2011

Sci. China Life Sci.

Self Cite

View full text Add to dashboard Cite

Trichophyton rubrum (T. rubrum) is a common superficial fungus. Molecular and genetic studies of T. rubrum are still limited. In this paper, we report the global analysis of gene expression profiles at different growth phases using cDNA microarray technology. A total of 2044 differentially expressed genes were obtained and clustered into three expression patterns. Our data confirmed previous results that many mRNAs were pre-stored in the conidia of T. rubrum. Transcriptional profiling and function analysis showed that some glycolytic enzymes share similar expression patterns and may be coregulated during the transition of growth phases. Some genes involved in small GTPase signaling pathways, and in cAMP-dependent and MAPK regulation pathways were induced in response to the growth dynamics of T. rubrum. Although the detailed biological roles of these T. rubrum genes are still unknown, our results suggest that these genes may be involved in regulation mechanisms in the life cycle of the fungus.

show abstract

Section: Discussionmentioning

confidence: 54%

“…A reference genomic DNA fragment (5 μg) isolated in the hyphal stage was labeled with Cy3 as previous report [13].…”

Section: Microarray Hybridizationmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Global gene expression profiles for the growth phases of Trichophyton rubrum

Liu

Leng

et al. 2011

Sci. China Life Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…bağlı olarak mantar yapısının değiştiğini göstermiştir 7 . T.rubrum kökenlerinin pre-germinasyon evresinde öncü olarak bekleyen genlerin, germinasyon ile birlikte aktifleşerek, eksternal ve/ veya abiyotik uyarılara yanıt, transkripsiyon, biyolojik işleyişin kontrolü, hücre haberleş-mesi, çoğalma, metabolizma, sinyal iletimi ve protein sentezi gibi farklı işlevlere sahip proteinleri eksprese ettiği gösterilmiştir 7,18 . Bu veriler ile karşılaştırılabilecek benzer araş-tırma sonuçları henüz T.mentagrophytes kompleks için bulunmamaktadır.…”

Section: Discussionunclassified

The Effect of Tween-80 on the Differentiation of Trichophyton mentagrophytes and Trichophyton rubrum Strains with FT-IR Spectroscopy

Ergin

İlkit²,

Gök³

et al. 2014

Mikrobiyol Bul

View full text Add to dashboard Cite

“…Several recent microarray studies have also investigated differentially expressed genes during germination in fungi, including Trichophyton rubrum (21), Neurospora crassa (16), and Ustilago maydis (50). Most of the genes upregulated in these organisms correspond to the same functional categories observed during B. emersonii germination, for instance, protein biosynthesis and primary metabolism involved in energy production of the cell.…”

Section: Vol 8 2009 Gene Expression During B Emersonii Germinationmentioning

confidence: 99%

Global Gene Expression Analysis during Germination in the Chytridiomycete Blastocladiella emersonii

et al. 2009

View full text Add to dashboard Cite

Blastocladiella emersonii is an aquatic fungus of the Chytridiomycete class. During germination, the zoospore, a motile nongrowing cell, goes through a cascade of morphological changes that culminates with its differentiation into the germling cell, capable of coenocytic vegetative growth. Transcriptome analyses of B. emersonii cells were carried out during germination induced under various environmental conditions. Microarray data analyzing 3,563 distinct B. emersonii genes revealed that 26% of them are differentially expressed during germination in nutrient medium at at least one of the time points investigated. Over 500 genes are upregulated during the time course of germination under those conditions, most being related to cell growth, including genes involved in protein biosynthesis, DNA transcription, energetic metabolism, carbohydrate and oligopeptide transport, and cell cycle control. On the other hand, several transcripts stored in the zoospores are downregulated during germination in nutrient medium, such as genes involved in signal transduction, amino acid transport, and chromosome organization. In addition, germination induced in the presence of nutrients was compared with that triggered either by adenine or potassium ions in inorganic salt solution. Several genes involved in cell growth, induced during germination in nutrient medium, do not show increased expression when B. emersonii zoospores germinate in inorganic solution, suggesting that nutrients exert a positive effect on gene transcription. The transcriptome data also revealed that most genes involved in cell signaling show the same expression pattern irrespective of the initial germination stimulus.

show abstract

The use of global transcriptional analysis to reveal the biological and cellular events involved in distinct development phases of Trichophyton rubrum conidial germination

Abstract: Background: Conidia are considered to be the primary cause of infections by Trichophyton rubrum.

Cited by 59 publications

References 51 publications

Global gene expression profiles for the growth phases of Trichophyton rubrum

Global gene expression profiles for the growth phases of Trichophyton rubrum

The Effect of Tween-80 on the Differentiation of Trichophyton mentagrophytes and Trichophyton rubrum Strains with FT-IR Spectroscopy

Global Gene Expression Analysis during Germination in the Chytridiomycete Blastocladiella emersonii

Contact Info

Product

Resources

About