The use of genetic engineering to obtain efficient production of porcine pancreatic phospholipase A2 by Saccharomyces cerevisiae

Production of recombinant notechis 11′2L, an enzymatically active mutant of a phospholipase A2 from Notechis scutatus scutatus venom, as directly generated by cleavage of a fusion protein produced in Escherichia coli

“…Production of recombinant PLA2s has been reported in yeast (Tanaka et al, 1988;Bekkers et al, 1991), in mammalian cells (Kramer et al, 1989;Pemas et al, 1991) and in E. coli (De Geus et al, 1987;Noel et al, 1991;Franken et al, 1992;Kelley et al, 1992). In E. coli, several approaches (0) were used to compete coated notechis 11'2 for binding to a monoclonal antibody recognizing a conserved epitope of PLA2s from Elapidae.…”

Phospholipases Used in Lipid Transformations

“…Mammalian pancreas and venoms from snakes and bees are the richest sources for secretory PLA 2 s, which have preferably been used for biocatalytic application (Table 1). PLA 2 from porcine pancreas has also been expressed in Saccharomyces cerevisiae (Bekkers et al, 1991). PLA 2 from porcine pancreas has also been expressed in Saccharomyces cerevisiae (Bekkers et al, 1991).…”

Enzymatic synthesis and modification of structured phospholipids: recent advances in enzyme preparation and biocatalytic processes

“…A number of attempts to increase their productivity have been made. For example, in the case of mammalian PLA 2 , (i) mammalian PLA 2 is heterologously expressed using microorganisms in which an efficient expression vector can be used (Bekkers et al 1991;Van den Bergh et al 1987) and (ii) porcine pancreatic PLA 2 fused to a glucoamylase is highly expressed in Aspergillus niger as a host, followed by processing to yield mature PLA 2 (Roberts et al 1992). However, the amounts of enzymes secreted extracellularly were less than 10 mg/L.…”