The use of fucosphere in the treatment of dermal burns in rabbits

Sezer, Ali Demir; Cevher, Erdal; Hatıpoğlu, Fatih; Oğurtan, Z.; Baş, Ahmet Levent; Akbuğa, Jülide

doi:10.1016/j.ejpb.2007.09.004

Cited by 45 publications

(36 citation statements)

References 32 publications

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“…These projections help to anchor the epidermis to the dermis. 44,45) Our results demonstrated the number of rete pegs in the 1300Ϯ109 1333Ϯ93 1455Ϯ64 111Ϯ10 96Ϯ2 162Ϯ10 121Ϯ8 14 1950Ϯ82 1941Ϯ107 2358Ϯ40 2086Ϯ134 154Ϯ5 136Ϯ3 261Ϯ16 187Ϯ9 21 3533Ϯ196 3666Ϯ152 5566Ϯ555 3586Ϯ149 134Ϯ8 162Ϯ21 189Ϯ23 146Ϯ10 group treated with fucoidan-chitosan hydrogel was higher than the other groups during treatment period (pϽ0.05) and reached highest level (8.3Ϯ2.8) on day 21 (Table 4, Fig. 6).…”

Section: Resultsmentioning

confidence: 75%

“…The highest number of the nuclear organized regions (NOR) in the epithelial cell nucleus, stating the division and proliferation of cells, were seen in the fucoidan-chitosan group on day 14 (8.5Ϯ0.1) when compared to other groups (pϽ0.05) ( Table 4) and this finding was consistent with the results of previous studies. 44,45) As a result, it was proved that the fucoidan-chitosan hydrogel was found highly effective to increase the healing and re-epithelization of the wound by day 21.…”

Section: Resultsmentioning

confidence: 98%

“…29) Polyelectrolyte complexes were also found to be more effective systems than either negatively charged polymer or positively charged polymer alone as wound dressing. 13) In this study, the mechanical properties such as hardness, cohesiveness and adhesiveness of gel formulations were investigated using texture profile analysis. 15,[30][31][32] The hardness test was performed to measure the required force to produce deformation of hydrogels.…”

Section: Resultsmentioning

confidence: 99%

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Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits

Sezer

Cevher

Hatıpoğlu

et al. 2008

Biological & Pharmaceutical Bulletin

157

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Burn healing includes a specific biological process related to the general phenomenon of growth and regeneration and the primary objective in burn care is the promotion of rapid wound healing with the best functional results. The principal function of a burn dressing is to provide an optimum healing milieu for natural healing and desirable burn dressing may, therefore, be characterized on the basis of its performance such as; (a) provision of adequate gaseous exchange, (b) provision and maintenance of a moist environment, (c) protection from infections and contamination, (d) absorption of wound fluids and (e) painless and easy removal. 1,2) Hydrogels are ideal biopolymeric pharmaceutical forms for the treatment of skin wounds. They have low interfacial tension, high molecular and oxygen permeability, good moisturizing and mechanical properties that resemble physiological soft tissue. 3,4) For this reasons, polysaccharides, e.g. chitosan, are having hydrogel forming properties have been considered to be advantageous in its application as a wound dressing material. 5) Chitosan, [a (1→4) 2-amino-2-deoxy-b-D-glucan], a unique polysaccharide derived from deacetylation of chitin, has been used in wound treatment owing to its good biocompatibility, biodegradability and accelerated granulation. [6][7][8] On the other hand, fucoidan is a sulphated polyfucose polysaccharide and has attracted considerable biotechnological research interest since the discovery that it possessed anti-coagulant activity similar to that of heparin and also reported to possess other properties including antithrombotic, anti-inflammatory, anti-tumoral and anti-viral effects. 9,10) Many of these effects are thought to be due to its interaction with growth factors such as basic fibroblast growth factor (bFGF) and transforming growth factor-b (TGF-b). Fucoidan may, therefore, be able to modulate growth factordependent pathways in the cell biology of tissue repair. 11) Although a great number of studies on different pharmacological properties of fucoidan and chitosan are present, there is little information on the fucoidan-based system used in burn healing and its only limited with cell culture. 12) The aim of this study was to prepare fucoidan-chitosan hydrogel and to investigate its treatment efficiency on dermal burns on rabbits. MATERIALS AND METHODSMaterials Chitosan (MW 250 kDa, deacetylation degree Ն90%, Pronova A/S, Norway; MW 400 kDa, deacetylation degree Ն60%, Fluka, Germany; MW 750 kDa, deacetylation degree Ն75%, Sigma, U.S.A.), Fucoidan (from Fucus vesiculosus) and lactic acid (85% m/v) purchased from Sigma, U.S.A. All other reagents used were of analytical grade.Preparation of Hydrogels The composition of gel formulations was given in Table 1. Fucoidan was dissolved in 1% m/v lactic acid solution by mechanical shaking at 300 rpm for 1 h. Then chitosan was left to swell in the fucoidan solution overnight to prepare hydrogels. 13) Formulations were kept at 4°C until experiment.Viscosity Measurement The viscosity of hydrogels was determine...

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Section: Resultsmentioning

confidence: 75%

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits

Sezer

Cevher

Hatıpoğlu

et al. 2008

Biological & Pharmaceutical Bulletin

157

View full text Add to dashboard Cite

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“…The authors reported values of 204.4 nm, 0.189, and +29.39 mV for MD, PI, and ZP, respectively. 47 Sezer et al 32 produced fucospheres by using polyelectrolyte complexation between CT and FC. In this report, the smaller fucosphere showed a larger mean diameter than NP2, as well as a higher PI and lower ZP than produced in this study.…”

Section: Resultsmentioning

confidence: 99%

“…When these are repulsed, the minimum radius for endocytosis increases, therefore hindering endocytosis. 30 The ionic interaction between CT and some anionic polysaccharides, such as CS, 14,31 FC, 32 and heparin, 33,34 has recently been used to produce micro-(CS) or nanoparticles (heparin) with biological activity. The production of these micro-(CS) and nanostructured materials is feasible through ionic interactions among the polyelectrolytes, ie, the positive charges of CT at acid pH interacting with the negatively charged sulfated polysaccharides (interpolymer complexation).…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity

Silva¹,

Garcia²,

Mori³

et al. 2012

IJN

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Abstract:The aim of this study was to produce and characterize nanoparticles (NPs), combining chondroitin sulfate (CS) and fucoidan (FC) with chitosan for therapeutic purposes. These NPs were characterized by dynamic light scattering, zeta potential determination, and transmission electronic microscopy. The anticoagulant activity was determined for FC NPs and compared with FC solution at the same concentration. FC NPs showed regular shapes and better anticoagulant activity than free polysaccharide solution. FC solution did not affect coagulation compared to FC NPs, which increased up to two-fold, even at a lower concentration. Cytotoxicity and permeability tests were conducted using Caco-2 cell monolayer, exhibiting no toxic effect in this cell line and higher permeability for NP2 samples than FC solution at the same concentration.

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Comparative effect of human andTrypanosoma cruzicalreticulin in wound healing

Arias

Sepúlveda

Bravo

et al. 2012

J Tissue Eng Regen Med

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In orthopaedics, the use of factors that enhance granulation tissue formation and prevent or delay new bone regeneration is sometimes desirable. Calreticulin (CRT), a unique endoplasmic reticulum luminal Ca(2+) -binding chaperone widely distributed in eukaryotic cells, is involved in many cellular functions. Among them, CRT has an important influence in cutaneous wound healing and diverse processes associated with cutaneous repair, inhibition of angiogenesis, promotion of cell adhesion and antitumour effect. One of the molecules involved in several aspects of the host-parasite interplay is Trypanosoma cruzi calreticulin (TcCRT), which is highly homologous to human calreticulin (HuCRT). Here, recombinant (r)HuCRT and rTcCRT are compared on their abilities to affect fibroblast behaviour in a scratch plate assay, and wound healing in in vivo skin rat models. In molar terms, rTcCRT is three orders of magnitude more efficient than rHuCRT in increasing proliferation and migration of human fibroblasts in vitro. A similar effect was observed in vivo on rat skin wounds and inhibition of bone gap bridging in rabbit unicortical bone osteotomies.

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The use of fucosphere in the treatment of dermal burns in rabbits

Cited by 45 publications

References 32 publications

Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits

Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits

Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity

Comparative effect of human andTrypanosoma cruzicalreticulin in wound healing

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